Preadsorbed Chymotrypsin Modulated the Composition of Protein Corona and Immunological Response

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2024 Jul 8

PMID 38973854

Authors

Ling Han

Yijing Wang

Hongyan Jia

Zhiqin Zhang

Shouning Yang

Fangxiao Li

Fengfeng Li

Huayan Yang

Affiliations

Soon will be listed here.

Abstract

It is well-known that proteins after administration into biological environments adsorb on the surface of nanoparticles (NPs). The biological identity could be determined by protein corona, but whether and how the preadsorbed molecules impact the composition of the corona and immunological response have rarely been reported. Here, the effects of preadsorbed chymotrypsin (Chy) on forming protein corona and subsequent immunological response are reported. We find that preadsorbed Chy on the surface of AuNPs results in a protein corona with enriched immunoglobulins and reduced human serum albumin protein, which further affect the polarization of macrophages into specific phenotypes. Our study suggests that the protein surrounding the nanoparticles could affect the protein corona and immunological response, which may direct the preparation of multifunctional nanomedicine for future studies.

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