Alpha 2.0
Login Register
» Articles » PMID: 3897272

Comparison of Measles Antihemolysin Test, Enzyme-linked Immunosorbent Assay, and Hemagglutination Inhibition Test with Neutralization Test for Determination of Immune Status

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1985 Aug 1
PMID 3897272
Citations 21
Authors
P W Neumann
J M Weber
A G Jessamine
M V OShaughnessy
Affiliations
Soon will be listed here.
Abstract

Sera were collected from 238 high-school students in Prince Edward Island for the determination of immune status before an anticipated measles outbreak. In addition, history of vaccination status and measles infection was obtained. In the subsequent outbreak, 28 students did contract measles. Specificity for hemagglutination inhibition (HI), antihemolysin (AH), and enzyme-linked immunosorbent assay (ELISA) was 100%, compared with the neutralization test. Corresponding sensitivity values for the tests were 66.0% (HI), 99.5% (AH), and 99.0% (ELISA). Predictive values for susceptibility were 26.9% (HI), 77.8% (AH), 75.7% (ELISA), 80% (neutralization), and 41.4% as determined by history of infection or vaccination. The predictive value for immunity as determined by history of previous infection or vaccination was 91.8%, compared with 100% for the four serological tests. No false-positive results were seen with any of these tests. Compared with the neutralization test, the HI test had 69 false-negative results, the AH had 1, and the ELISA test had 2. The AH and ELISA tests provided sensitive and specific alternatives to the commonly used HI test for immune status determination.

Citing Articles

Undernutrition and antibody response to measles, tetanus and Haemophilus Influenzae type b (Hib) vaccination in pre-school south African children: The VHEMBE birth cohort study.

Eskenazi B, Rauch S, Elsiwi B, Bornman R, Obida M, Brewer A Vaccine. 2024; 46():126564.

PMID: 39665976 PMC: 11750586. DOI: 10.1016/j.vaccine.2024.126564.

Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips.

Nam S, Jeon D, Yoon Y, Lee G, Chang Y, Won D Biosensors (Basel). 2023; 13(7).

PMID: 37504130 PMC: 10377501. DOI: 10.3390/bios13070733.

Correlating IgG Levels with Neutralising Antibody Levels to Indicate Clinical Protection in Healthcare Workers at Risk during a Measles Outbreak.

Hu S, Logan N, Coleman S, Evans C, Willett B, Hosie M Viruses. 2022; 14(8).

PMID: 36016338 PMC: 9415042. DOI: 10.3390/v14081716.

Evaluation of the effect of maternally derived antibody on response to MMR vaccine in Thai infants.

Hu S, Logan N, Puenpa J, Wanlapakorn N, Vongpunsawad S, Poovorawan Y Vaccine. 2022; 40(10):1439-1447.

PMID: 35135700 PMC: 8884255. DOI: 10.1016/j.vaccine.2022.01.049.

Effect of age at vaccination on the measles vaccine effectiveness and immunogenicity: systematic review and meta-analysis.

Carazo S, Billard M, Boutin A, De Serres G BMC Infect Dis. 2020; 20(1):251.

PMID: 32223757 PMC: 7104533. DOI: 10.1186/s12879-020-4870-x.

References
1.
Norrby E, Gollmar Y . Appearance and persistence of antibodies against different virus components after regular measles infections. Infect Immun. 1972; 6(3):240-7. PMC: 422522. DOI: 10.1128/iai.6.3.240-247.1972. View
2.
Norrby E, Gollmar Y . Identification of measles virus-specific hemolysis-inihibiting antibodies separate from hemagglutination-inhibiting antibodies. Infect Immun. 1975; 11(2):231-9. PMC: 415050. DOI: 10.1128/iai.11.2.231-239.1975. View
3.
Norrby E, LAGERCRANTZ R . Measles vaccination. VIII. The occurrence of antibodies against virus envelope components after immunization with inactivated vaccine. Effects of revaccination with live measles vaccine. Acta Paediatr Scand. 1976; 65(2):171-6. DOI: 10.1111/j.1651-2227.1976.tb16532.x. View
4.
Yeager A, Davis J, Ross L, Harvey B . Measles immunization. Successes and failures. JAMA. 1977; 237(4):347-51. View
5.
Ransohoff D, Feinstein A . Problems of spectrum and bias in evaluating the efficacy of diagnostic tests. N Engl J Med. 1978; 299(17):926-30. DOI: 10.1056/NEJM197810262991705. View