Genome-wide Analysis of Wheat Xyloglucan Endotransglucosylase/hydrolase (XTH) Gene Family Revealed TaXTH17 Involved in Abiotic Stress Responses

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2024 Jul 6

PMID 38971763

Authors

Huihui Bi

Zeliang Liu

Shanshan Liu

Wenchen Qiao

Kunpu Zhang

Minghui Zhao

Daowen Wang

Affiliations

Soon will be listed here.

Abstract

Background: Environmental stresses, including high salinity and drought, severely diminish wheat yield and quality globally. The xyloglucan endotransglucosylase/hydrolase (XTH) family represents a class of cell wall-modifying enzymes and plays important roles in plants growth, development and stress adaptation. However, systematic analyses of XTH family genes and their functions under salt and drought stresses have not been undertaken in wheat.

Results: In this study, we identified a total of 135 XTH genes in wheat, which were clustered into three evolutionary groups. These TaXTHs were unevenly distributed on 21 chromosomes of wheat with a majority of TaXTHs located on homelogous groups 2, 3 and 7. Gene duplication analysis revealed that segmental and tandem duplication were the main reasons for the expansion of XTH family in wheat. Interaction network predictions indicated that TaXTHs could interact with multiple proteins, including three kinases, one methyltransferase and one gibberellin-regulated protein. The promoters of the TaXTH genes harbored various cis-acting elements related to stress and hormone responses. RNA-seq data analyses showed that some TaXTH genes were induced by salt and drought stresses. Furthermore, we verified that TaXTH17 was induced by abiotic stresses and phytohormone treatments, and demonstrated that TaXTH17 was localized in the secretory pathway and cell wall. Functional analyses conducted in heterologous expression systems and in wheat established that TaXTH17 plays a negative role in plant resistance to salt and drought.

Conclusions: We identified 135 XTH genes in wheat and conducted comprehensive analyses of their phylogenetic relationships, gene structures, conserved motifs, gene duplication events, chromosome locations, interaction networks, cis-acting elements and gene expression patterns. Furthermore, we provided solid evidence supporting the notion that TaXTH17 plays a negative role in plant resistance to salt and drought stresses. Collectively, our results provide valuable insights into understanding wheat XTHs, particularly their involvement in plant stress responses, and establish a foundation for further functional and mechanistic studies of TaXTHs.

Citing Articles

Genome-Wide Identification of Xyloglucan Endotransglucosylase/Hydrolase Multigene Family in Chinese Jujube () and Their Expression Patterns Under Different Environmental Stresses.

Refaiy M, Tahir M, Jiao L, Zhang X, Zhang H, Chen Y Plants (Basel). 2025; 13(24.

PMID: 39771201 PMC: 11677919. DOI: 10.3390/plants13243503.

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