Genetic Variability of Human Papillomavirus Type 18 Based on E6, E7 and L1 Genes in Central China

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2024 Jul 5

PMID 38970084

Authors

Ting Li

Zhiping Yang

Ping Luo

Yang Yang

Zicong Lin

Bing Mei

Affiliations

Soon will be listed here.

Abstract

Background: High-risk human papillomavirus (HR-HPV) infection is an important factor for the development of cervical cancer. HPV18 is the second most common HR-HPV after HPV16.

Methods: In this study, MEGA11 software was used to analyze the variation and phylogenetic tree of HPV18 E6-E7 and L1 genes. The selective pressure to E6, E7 and L1 genes was estimated using pamlX. In addition, the B cell epitopes of L1 amino acid sequences and T cell epitopes of E6-E7 amino acid sequences in HPV18 were predicted by ABCpred server and IEDB website, respectively.

Results: A total of 9 single nucleotide variants were found in E6-E7 sequences, of which 2 were nonsynonymous variants and 7 were synonymous variants. Twenty single nucleotide variants were identified in L1 sequence, including 11 nonsynonymous variants and 9 synonymous variants. Phylogenetic analysis showed that E6-E7 and L1 sequences were all distributed in A lineage. In HPV18 E6, E7 and L1 sequences, no positively selected site was found. The nonconservative substitution R545C in L1 affected hypothetical B cell epitope. Two nonconservative substitutions, S82A in E6, and R53Q in E7, impacted multiple hypothetical T cell epitopes.

Conclusion: The sequence variation data of HPV18 may lay a foundation for the virus diagnosis, further study of cervical cancer and vaccine design in central China.

Citing Articles

Novel dual-pathogen multi-epitope mRNA vaccine development for Brucella melitensis and Mycobacterium tuberculosis in silico approach.

Zhu Y, Shi J, Wang Q, Zhu Y, Li M, Tian T PLoS One. 2024; 19(10):e0309560.

PMID: 39466745 PMC: 11515988. DOI: 10.1371/journal.pone.0309560.

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