Overexpression of , an F-box Gene from , Increased the Plant Branching in Tobacco and Rice

Overview

Journal Plant Direct

Specialty Biology

Date 2024 Jul 4

PMID 38962172

Authors

Bokun Zhou

Qi Sheng

Xinzhuan Yao

Tong Li

Litang Lu

Affiliations

Soon will be listed here.

Abstract

Tea plant ( [.]) is one of the most important crops in China, and tea branch is an important agronomic trait that determines the yield of tea plant. In previous work focused on GWAS that detecting GWAS signals related to plant architecture through whole genome re-sequencing of ancient tea plants, a gene locus TEA 029928 significantly related to plant type was found. Sequence alignment results showed that this gene belonged to the F-box family. We named it . CsBRC-GFP fusion proteins were mainly localized in the plasma membrane. By comparing the phenotypes of transgenic tobacco and WT tobacco, it was found that the number of branches of transgenic tobacco was significantly higher than that of wild-type tobacco. Through RNA-seq analysis, it was found that affects the branching development of plants by regulating the expression of genes related to brassinosteroid synthesis pathway in plants. In addition, overexpression of in rice could increase tiller number, grain length and width, and 1,000-grain weight.

References

Zhu J, Li Y, Cao D, Yang H, Oh E, Bi Y . The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in Arabidopsis. Mol Cell. 2017; 66(5):648-657.e4. PMC: 5935450. DOI: 10.1016/j.molcel.2017.05.012. View

Olszewski N, Sun T, Gubler F . Gibberellin signaling: biosynthesis, catabolism, and response pathways. Plant Cell. 2002; 14 Suppl:S61-80. PMC: 151248. DOI: 10.1105/tpc.010476. View

Chen Y, Fan X, Song W, Zhang Y, Xu G . Over-expression of OsPIN2 leads to increased tiller numbers, angle and shorter plant height through suppression of OsLAZY1. Plant Biotechnol J. 2011; 10(2):139-49. DOI: 10.1111/j.1467-7652.2011.00637.x. View

Lu L, Chen H, Wang X, Zhao Y, Yao X, Xiong B . Genome-level diversification of eight ancient tea populations in the Guizhou and Yunnan regions identifies candidate genes for core agronomic traits. Hortic Res. 2021; 8(1):190. PMC: 8355299. DOI: 10.1038/s41438-021-00617-9. View

Gendron J, Wang Z . Multiple mechanisms modulate brassinosteroid signaling. Curr Opin Plant Biol. 2007; 10(5):436-41. PMC: 2093957. DOI: 10.1016/j.pbi.2007.08.015. View

Tong H, Jin Y, Liu W, Li F, Fang J, Yin Y . DWARF AND LOW-TILLERING, a new member of the GRAS family, plays positive roles in brassinosteroid signaling in rice. Plant J. 2009; 58(5):803-16. DOI: 10.1111/j.1365-313X.2009.03825.x. View

Leyser O . Regulation of shoot branching by auxin. Trends Plant Sci. 2003; 8(11):541-5. DOI: 10.1016/j.tplants.2003.09.008. View

Fujita S, Ohnishi T, Watanabe B, Yokota T, Takatsuto S, Fujioka S . Arabidopsis CYP90B1 catalyses the early C-22 hydroxylation of C27, C28 and C29 sterols. Plant J. 2006; 45(5):765-74. DOI: 10.1111/j.1365-313X.2005.02639.x. View

Dharmasiri N, Dharmasiri S, Weijers D, Lechner E, Yamada M, Hobbie L . Plant development is regulated by a family of auxin receptor F box proteins. Dev Cell. 2005; 9(1):109-19. DOI: 10.1016/j.devcel.2005.05.014. View

10.

He Q, Yang L, Hu W, Zhang J, Xing Y . Overexpression of an auxin receptor OsAFB6 significantly enhanced grain yield by increasing cytokinin and decreasing auxin concentrations in rice panicle. Sci Rep. 2018; 8(1):14051. PMC: 6145926. DOI: 10.1038/s41598-018-32450-x. View

11.

Jiao Y, Wang Y, Xue D, Wang J, Yan M, Liu G . Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice. Nat Genet. 2010; 42(6):541-4. DOI: 10.1038/ng.591. View

12.

Hillman J, Math V, Medlow G . Apical dominance and the levels of indole acetic acid in Phaseolus lateral buds. Planta. 2014; 134(2):191-3. DOI: 10.1007/BF00384970. View

13.

Petrasek J, Mravec J, Bouchard R, Blakeslee J, Abas M, Seifertova D . PIN proteins perform a rate-limiting function in cellular auxin efflux. Science. 2006; 312(5775):914-8. DOI: 10.1126/science.1123542. View

14.

Durbak A, Yao H, McSteen P . Hormone signaling in plant development. Curr Opin Plant Biol. 2012; 15(1):92-6. DOI: 10.1016/j.pbi.2011.12.004. View

15.

Guo D, Kong S, Chu X, Li X, Pan H . De Novo Biosynthesis of Indole-3-acetic Acid in Engineered . J Agric Food Chem. 2019; 67(29):8186-8190. DOI: 10.1021/acs.jafc.9b02048. View

16.

Gao L, Jia S, Cao L, Ma Y, Wang J, Lan D . An F-box protein from wheat, TaFBA-2A, negatively regulates JA biosynthesis and confers improved salt tolerance and increased JA responsiveness to transgenic rice plants. Plant Physiol Biochem. 2022; 182:227-239. DOI: 10.1016/j.plaphy.2022.04.025. View

17.

Zhao L, Tan L, Zhu Z, Xiao L, Xie D, Sun C . PAY1 improves plant architecture and enhances grain yield in rice. Plant J. 2015; 83(3):528-36. PMC: 4758413. DOI: 10.1111/tpj.12905. View

18.

Cardozo T, Pagano M . The SCF ubiquitin ligase: insights into a molecular machine. Nat Rev Mol Cell Biol. 2004; 5(9):739-51. DOI: 10.1038/nrm1471. View

19.

Umehara M, Hanada A, Yoshida S, Akiyama K, Arite T, Takeda-Kamiya N . Inhibition of shoot branching by new terpenoid plant hormones. Nature. 2008; 455(7210):195-200. DOI: 10.1038/nature07272. View

20.

Zhang N, Yu H, Yu H, Cai Y, Huang L, Xu C . A Core Regulatory Pathway Controlling Rice Tiller Angle Mediated by the -Dependent Asymmetric Distribution of Auxin. Plant Cell. 2018; 30(7):1461-1475. PMC: 6096585. DOI: 10.1105/tpc.18.00063. View