Development of a Colloidal Gold Immunochromatographic Strip for the Simultaneous Detection of Porcine Epidemic Diarrhea Virus and Transmissible Gastroenteritis Virus

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Jul 4

PMID 38962124

Authors

Jinzhu Zhou

Wei Wu

Dandan Wang

Wei Wang

Xinjian Chang

Yunchuan Li

Jizong Li

Baochao Fan

Junming Zhou

Rongli Guo

Xuejiao Zhu

Bin Li

Affiliations

Soon will be listed here.

Abstract

In recent years, porcine diarrhea-associated viruses have caused significant economic losses globally. These viruses present similar clinical symptoms, such as watery diarrhea, dehydration, and vomiting. Co-infections with porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are common. For the rapid and on-site preliminary diagnosis on the pig farms, this study aimed to develop a colloidal gold immunochromatography assay (GICA) strip for the detection of PEDV and TGEV simultaneously. The GICA kit showed that there was no cross-reactivity with the other five common porcine viruses. With visual observation, the lower limits were approximately 104 TCID50/mL and 104 TCID50/mL for PEDV and TGEV, respectively. The GICA strip could be stored at 4°C or 25°C for 12 months without affecting its efficacy. To validate the GICA strip, 121 clinical samples were tested. The positive rates of PEDV and TGEV were 42.9 and 9.9%, respectively, and the co-infection rate of the two viruses was 5.8% based on the duplex GICA strip. Thus, the established GICA strip is a rapid, specific, and stable tool for on-site preliminary diagnosis of PEDV- and TGEV-associated diarrhea.

Citing Articles

Development and Immunogenicity Study of Subunit Vaccines Based on Spike Proteins of Porcine Epidemic Diarrhea Virus and Porcine Transmissible Gastroenteritis Virus.

Xu M, Yang Z, Yang N, Li H, Ma H, Yi J Vet Sci. 2025; 12(2).

PMID: 40005866 PMC: 11860644. DOI: 10.3390/vetsci12020106.

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