A Mini Review: The Potential Biomarkers for Non-invasive Diagnosis of Pulpal Inflammation

Overview

Journal Front Dent Med

Specialty Dentistry

Date 2024 Jul 1

PMID 38947881

Authors

Brahmleen Kaur

Yoshifumi Kobayashi

Carla Cugini

Emi Shimizu

Affiliations

Soon will be listed here.

Abstract

For assessing the adequacy of vital pulp therapy for an inflamed pulp, the use of non-invasive diagnostic tools is necessary to avoid further damage to the teeth. Detection of biomarkers that are indicative of the inflammatory status in pulp can be a promising tool for this purpose. These biomarkers need to be reliably correlated with pulpal inflammation and to be easily detected without pulp exposure. This mini-review article aims to review biomarkers that are present in gingival crevicular fluid (GCF) in inflamed pulp conditions. Several studies have reported the availability of various biomarkers including cytokines, proteases, elastase, neuropeptides, and growth factors. Non-invasive pulpal diagnostic methods will be useful as well to determine reversibility, irreversibility, or necrosis of inflamed pulp. These types of molecular diagnoses analyzing the proteome have revolutionized the medical field, and are one of the most promising empirical methodologies that a clinician can utilize for the proactive identification of pulpal disease.

Citing Articles

Outcome assessment methods of bioactive and biodegradable materials as pulpotomy agents in primary and permanent teeth: a scoping review.

Elhamouly Y, Adham M, Dowidar K, El Backly R BMC Oral Health. 2024; 24(1):496.

PMID: 38678210 PMC: 11055312. DOI: 10.1186/s12903-024-04221-w.

References

Tsuchida S . Proteome Analysis of Molecular Events in Oral Pathogenesis and Virus: A Review with a Particular Focus on Periodontitis. Int J Mol Sci. 2020; 21(15). PMC: 7432693. DOI: 10.3390/ijms21155184. View

Cervino G, Fiorillo L, Herford A, Romeo U, Bianchi A, Crimi S . Molecular Biomarkers Related to Oral Carcinoma: Clinical Trial Outcome Evaluation in a Literature Review. Dis Markers. 2019; 2019:8040361. PMC: 6452537. DOI: 10.1155/2019/8040361. View

Kokkas A, Goulas A, Varsamidis K, Mirtsou V, Tziafas D . Irreversible but not reversible pulpitis is associated with up-regulation of tumour necrosis factor-alpha gene expression in human pulp. Int Endod J. 2007; 40(3):198-203. DOI: 10.1111/j.1365-2591.2007.01215.x. View

Sattari M, Haghighi A, Tamijani H . The relationship of pulp polyp with the presence and concentration of immunoglobulin E, histamine, interleukin-4 and interleukin-12. Aust Endod J. 2009; 35(3):164-8. DOI: 10.1111/j.1747-4477.2009.00160.x. View

Tancharoen S, Tengrungsun T, Suddhasthira T, Kikuchi K, Vechvongvan N, Tokuda M . Overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation. Mediators Inflamm. 2014; 2014:754069. PMC: 4121219. DOI: 10.1155/2014/754069. View

Park S, Ye L, Love R, Farges J, Yumoto H . Inflammation of the Dental Pulp. Mediators Inflamm. 2016; 2015:980196. PMC: 4736221. DOI: 10.1155/2015/980196. View

Aral K, Berdeli E, Cooper P, Milward M, Kapila Y, Karadede Unal B . Differential expression of inflammasome regulatory transcripts in periodontal disease. J Periodontol. 2019; 91(5):606-616. DOI: 10.1002/JPER.19-0222. View

Lin S, Yen-Ping Kuo M, Wang T, Wang J, Yeh F, Hsiao M . Sequential expressions of MMP-1, TIMP-1, IL-6, and COX-2 genes in induced periapical lesions in rats. Eur J Oral Sci. 2002; 110(3):246-53. DOI: 10.1034/j.1600-0447.2002.11227.x. View

Linden G, McKinnell J, Shaw C, Lundy F . Substance P and neurokinin A in gingival crevicular fluid in periodontal health and disease. J Clin Periodontol. 1997; 24(11):799-803. DOI: 10.1111/j.1600-051x.1997.tb01192.x. View

10.

Cohen J, Reader A, Fertel R, Beck M, Meyers W . A radioimmunoassay determination of the concentrations of prostaglandins E2 and F2alpha in painful and asymptomatic human dental pulps. J Endod. 1985; 11(8):330-5. DOI: 10.1016/s0099-2399(85)80039-x. View

11.

Lundy F, Mullally B, Burden D, Lamey P, Shaw C, Linden G . Changes in substance P and neurokinin A in gingival crevicular fluid in response to periodontal treatment. J Clin Periodontol. 2000; 27(7):526-30. DOI: 10.1034/j.1600-051x.2000.027007526.x. View

12.

Huang F, Tsai C, Chen Y, Liu C, Chou M, Chang Y . Upregulation of tissue-type plasminogen activator in inflamed human dental pulps. Int Endod J. 2005; 38(5):328-33. DOI: 10.1111/j.1365-2591.2005.00951.x. View

13.

Caviedes-Bucheli J, Lombana N, Azuero-Holguin M, Munoz H . Quantification of neuropeptides (calcitonin gene-related peptide, substance P, neurokinin A, neuropeptide Y and vasoactive intestinal polypeptide) expressed in healthy and inflamed human dental pulp. Int Endod J. 2006; 39(5):394-400. DOI: 10.1111/j.1365-2591.2006.01093.x. View

14.

Karapanou V, Kempuraj D, Theoharides T . Interleukin-8 is increased in gingival crevicular fluid from patients with acute pulpitis. J Endod. 2008; 34(2):148-51. DOI: 10.1016/j.joen.2007.10.022. View

15.

Yang G, Shao G . Elevated serum IL-11, TNF α, and VEGF expressions contribute to the pathophysiology of hypertensive intracerebral hemorrhage (HICH). Neurol Sci. 2016; 37(8):1253-9. DOI: 10.1007/s10072-016-2576-z. View

16.

Sun X, Meng L, Qiao W, Yang R, Gao Q, Peng Y . Vascular endothelial growth factor A/Vascular endothelial growth factor receptor 2 axis promotes human dental pulp stem cell migration via the FAK/PI3K/Akt and p38 MAPK signalling pathways. Int Endod J. 2019; 52(12):1691-1703. DOI: 10.1111/iej.13179. View

17.

Heinrich P, Castell J, Andus T . Interleukin-6 and the acute phase response. Biochem J. 1990; 265(3):621-36. PMC: 1133681. DOI: 10.1042/bj2650621. View

18.

Hemadi A, Huang R, Zhou Y, Zou J . Salivary proteins and microbiota as biomarkers for early childhood caries risk assessment. Int J Oral Sci. 2017; 9(11):e1. PMC: 5775330. DOI: 10.1038/ijos.2017.35. View

19.

Trowbridge H . Pathogenesis of pulpitis resulting from dental caries. J Endod. 1981; 7(2):52-60. DOI: 10.1016/s0099-2399(81)80242-7. View

20.

Abd-Elmeguid A, Abdeldayem M, Kline L, Moqbel R, Vliagoftis H, Yu D . Osteocalcin expression in pulp inflammation. J Endod. 2013; 39(7):865-72. DOI: 10.1016/j.joen.2012.12.035. View