The Regulatory Role of MA Modification in the Maintenance and Differentiation of Embryonic Stem Cells

Overview

Journal Genes Dis

Date 2024 Jul 1

PMID 38947741

Authors

Jin Zhang

Lingling Tong

Yuchen Liu

Xiang Li

Jiayi Wang

Ruoxin Lin

Ziyu Zhou

Yunbing Chen

Yanxi Chen

Yirong Liu

Di Chen

Affiliations

Soon will be listed here.

Abstract

As the most prevalent and reversible internal epigenetic modification in eukaryotic mRNAs, -methyladenosine (mA) post-transcriptionally regulates the processing and metabolism of mRNAs involved in diverse biological processes. mA modification is regulated by mA writers, erasers, and readers. Emerging evidence suggests that mA modification plays essential roles in modulating the cell-fate transition of embryonic stem cells. Mechanistic investigation of embryonic stem cell maintenance and differentiation is critical for understanding early embryonic development, which is also the premise for the application of embryonic stem cells in regenerative medicine. This review highlights the current knowledge of mA modification and its essential regulatory contribution to the cell fate transition of mouse and human embryonic stem cells.

References

Shi H, Wei J, He C . Where, When, and How: Context-Dependent Functions of RNA Methylation Writers, Readers, and Erasers. Mol Cell. 2019; 74(4):640-650. PMC: 6527355. DOI: 10.1016/j.molcel.2019.04.025. View

Sun H, Zhu A, Gao Y, Terajima H, Fei Q, Liu S . Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases mA Methylation. Mol Cell. 2020; 80(4):633-647.e7. PMC: 7720844. DOI: 10.1016/j.molcel.2020.10.026. View

Wang Y, Li Y, Toth J, Petroski M, Zhang Z, Zhao J . N6-methyladenosine modification destabilizes developmental regulators in embryonic stem cells. Nat Cell Biol. 2014; 16(2):191-8. PMC: 4640932. DOI: 10.1038/ncb2902. View

Wen J, Lv R, Ma H, Shen H, He C, Wang J . Zc3h13 Regulates Nuclear RNA mA Methylation and Mouse Embryonic Stem Cell Self-Renewal. Mol Cell. 2018; 69(6):1028-1038.e6. PMC: 5858226. DOI: 10.1016/j.molcel.2018.02.015. View

Knuckles P, Lence T, Haussmann I, Jacob D, Kreim N, Carl S . Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the mA machinery component Wtap/Fl(2)d. Genes Dev. 2018; 32(5-6):415-429. PMC: 5900714. DOI: 10.1101/gad.309146.117. View

PERRY R, Kelley D, Friderici K, Rottman F . The methylated constituents of L cell messenger RNA: evidence for an unusual cluster at the 5' terminus. Cell. 1975; 4(4):387-94. DOI: 10.1016/0092-8674(75)90159-2. View

Xu C, Liu K, Ahmed H, Loppnau P, Schapira M, Min J . Structural Basis for the Discriminative Recognition of N6-Methyladenosine RNA by the Human YT521-B Homology Domain Family of Proteins. J Biol Chem. 2015; 290(41):24902-13. PMC: 4598999. DOI: 10.1074/jbc.M115.680389. View

Chelmicki T, Roger E, Teissandier A, Dura M, Bonneville L, Rucli S . mA RNA methylation regulates the fate of endogenous retroviruses. Nature. 2021; 591(7849):312-316. DOI: 10.1038/s41586-020-03135-1. View

Meng T, Lu X, Guo L, Hou G, Ma X, Li Q . Mettl14 is required for mouse postimplantation development by facilitating epiblast maturation. FASEB J. 2018; 33(1):1179-1187. DOI: 10.1096/fj.201800719R. View

10.

Brons I, Smithers L, Trotter M, Rugg-Gunn P, Sun B, Chuva de Sousa Lopes S . Derivation of pluripotent epiblast stem cells from mammalian embryos. Nature. 2007; 448(7150):191-5. DOI: 10.1038/nature05950. View

11.

Chen C, Liu W, Guo J, Liu Y, Liu X, Liu J . Nuclear mA reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos. Protein Cell. 2021; 12(6):455-474. PMC: 8160034. DOI: 10.1007/s13238-021-00837-8. View

12.

Chen J, Zhang Y, Huang C, Shen H, Sun B, Cheng X . mA Regulates Neurogenesis and Neuronal Development by Modulating Histone Methyltransferase Ezh2. Genomics Proteomics Bioinformatics. 2019; 17(2):154-168. PMC: 6620265. DOI: 10.1016/j.gpb.2018.12.007. View

13.

Yang D, Qiao J, Wang G, Lan Y, Li G, Guo X . N6-Methyladenosine modification of lincRNA 1281 is critically required for mESC differentiation potential. Nucleic Acids Res. 2018; 46(8):3906-3920. PMC: 5934679. DOI: 10.1093/nar/gky130. View

14.

Davidson K, Mason E, Pera M . The pluripotent state in mouse and human. Development. 2015; 142(18):3090-9. DOI: 10.1242/dev.116061. View

15.

Lee J, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen P . Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation. Mol Cell. 2021; 81(16):3368-3385.e9. PMC: 8383322. DOI: 10.1016/j.molcel.2021.07.024. View

16.

Geula S, Moshitch-Moshkovitz S, Dominissini D, AlFatah Mansour A, Kol N, Salmon-Divon M . Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation. Science. 2015; 347(6225):1002-6. DOI: 10.1126/science.1261417. View

17.

Weng H, Huang F, Yu Z, Chen Z, Prince E, Kang Y . The mA reader IGF2BP2 regulates glutamine metabolism and represents a therapeutic target in acute myeloid leukemia. Cancer Cell. 2022; 40(12):1566-1582.e10. PMC: 9772162. DOI: 10.1016/j.ccell.2022.10.004. View

18.

Li Y, Xia L, Tan K, Ye X, Zuo Z, Li M . N-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2. Nat Genet. 2020; 52(9):870-877. DOI: 10.1038/s41588-020-0677-3. View

19.

Nichols J, Smith A . Naive and primed pluripotent states. Cell Stem Cell. 2009; 4(6):487-92. DOI: 10.1016/j.stem.2009.05.015. View

20.

Thomson J, Itskovitz-Eldor J, Shapiro S, Waknitz M, Swiergiel J, Marshall V . Embryonic stem cell lines derived from human blastocysts. Science. 1998; 282(5391):1145-7. DOI: 10.1126/science.282.5391.1145. View