Comparison of the PRAS II, AN-Ident, and RapID-ANA Systems for Identification of Anaerobic Bacteria

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1985 Jul 1

PMID 3894412

Citations 17

Authors

R S Burlage

P D Ellner

Affiliations

Soon will be listed here.

Abstract

Two rapid systems for the identification of anaerobes were compared to a conventional growth system aided by a computer. The rapid systems (AN-Ident and RapID-ANA) are non-growth-dependent micromethods that identify anaerobes in 4 h by the action of various constitutive enzymes on chromogenic substrates. The organisms tested were 98 anaerobes, most of which were clinical isolates. The AN-Ident system identified 76 of these to species level and 86 to genus level; the RapID-ANA system correctly identified 74 of the organisms to species level and identified 93 to genus level. The PRAS II system correctly identified 77 to species level and 96 to genus level. In most instances, adequate identification could be obtained with either of the two rapid systems, but the conventional PRAS II system remains the most accurate.

Citing Articles

Discrimination of Human Pathogen Clostridium Species Especially of the Heterogeneous C. sporogenes and C. botulinum by MALDI-TOF Mass Spectrometry.

Schaumann R, Dallacker-Losensky K, Rosenkranz C, Genzel G, Stingu C, Schellenberger W Curr Microbiol. 2018; 75(11):1506-1515.

PMID: 30120528 DOI: 10.1007/s00284-018-1552-7.

Laboratory diagnostics of botulism.

Lindstrom M, Korkeala H Clin Microbiol Rev. 2006; 19(2):298-314.

PMID: 16614251 PMC: 1471988. DOI: 10.1128/CMR.19.2.298-314.2006.

Evaluation of the BBL Crystal Anaerobe identification system.

Cavallaro J, Wiggs L, Miller J J Clin Microbiol. 1997; 35(12):3186-91.

PMID: 9399517 PMC: 230145. DOI: 10.1128/jcm.35.12.3186-3191.1997.

Identification and antimicrobial resistance patterns of clinical isolates of Clostridium clostridioforme, Clostridium innocuum, and Clostridium ramosum compared with those of clinical isolates of Clostridium perfringens.

Alexander C, Citron D, Brazier J, Goldstein E J Clin Microbiol. 1995; 33(12):3209-15.

PMID: 8586704 PMC: 228675. DOI: 10.1128/jcm.33.12.3209-3215.1995.

Evaluation of an automated system for identification of anaerobic bacteria.

Arzese A, Minisini R, Botta G Eur J Clin Microbiol Infect Dis. 1994; 13(2):135-41.

PMID: 8013485 DOI: 10.1007/BF01982186.

References

Starr S, Thompson F, Dowell Jr V, BALOWS A . Micromethod system for identification of anaerobic bacteria. Appl Microbiol. 1973; 25(5):713-7. PMC: 380900. DOI: 10.1128/am.25.5.713-717.1973. View

Ellner P, Granato P, May C . Recovery and identification of anaerobes: a system suitable for the routine clinical laboratory. Appl Microbiol. 1973; 26(6):904-13. PMC: 379932. DOI: 10.1128/am.26.6.904-913.1973. View

Savuto P, Ellner P . Evaluation of a microtiter system for identification of anaerobic bacteria. J Clin Microbiol. 1984; 20(1):81-3. PMC: 271251. DOI: 10.1128/jcm.20.1.81-83.1984. View

Tharagonnet D, Sisson P, Roxby C, Ingham H, Selkon J . The API ZYM system in the identification of Gram-negative anaerobes. J Clin Pathol. 1977; 30(6):505-9. PMC: 476458. DOI: 10.1136/jcp.30.6.505. View

Beaucage C, Onderdonk A . Evaluation of a prereduced anaerobically sterilized medium (PRAS II) system for identification anaerobic microorganisms. J Clin Microbiol. 1982; 16(3):570-2. PMC: 272415. DOI: 10.1128/jcm.16.3.570-572.1982. View