Twilight Length Alters Growth and Flowering Time in Arabidopsis Via /

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2024 Jun 28

PMID 38941453

Authors

Devang Mehta

Sabine Scandola

Curtis Kennedy

Christina Lummer

Maria Camila Rodriguez Gallo

Lauren E Grubb

Maryalle Tan

Enrico Scarpella

R Glen Uhrig

Affiliations

Soon will be listed here.

Abstract

Decades of research have uncovered how plants respond to two environmental variables that change across latitudes and over seasons: photoperiod and temperature. However, a third such variable, twilight length, has so far gone unstudied. Here, using controlled growth setups, we show that the duration of twilight affects growth and flowering time via the clock genes in the model plant Arabidopsis. Using a series of progressively truncated no-twilight photoperiods, we also found that plants are more sensitive to twilight length compared to equivalent changes in solely photoperiods. Transcriptome and proteome analyses showed that twilight length affects reactive oxygen species metabolism, photosynthesis, and carbon metabolism. Genetic analyses suggested a twilight sensing pathway from the photoreceptors , , , and through to flowering modulation through the pathway. Overall, our findings call for more nuanced models of day-length perception in plants and posit that twilight is an important determinant of plant growth and development.

References

Sloat L, Davis S, Gerber J, Moore F, Ray D, West P . Climate adaptation by crop migration. Nat Commun. 2020; 11(1):1243. PMC: 7060181. DOI: 10.1038/s41467-020-15076-4. View

Guo H, Yang H, Mockler T, Lin C . Regulation of flowering time by Arabidopsis photoreceptors. Science. 1998; 279(5355):1360-3. DOI: 10.1126/science.279.5355.1360. View

Liao Y, Smyth G, Shi W . The Subread aligner: fast, accurate and scalable read mapping by seed-and-vote. Nucleic Acids Res. 2013; 41(10):e108. PMC: 3664803. DOI: 10.1093/nar/gkt214. View

Gehan M, Fahlgren N, Abbasi A, Berry J, Callen S, Chavez L . PlantCV v2: Image analysis software for high-throughput plant phenotyping. PeerJ. 2017; 5:e4088. PMC: 5713628. DOI: 10.7717/peerj.4088. View

Devlin P, Robson P, Patel S, Goosey L, Sharrock R, Whitelam G . Phytochrome D acts in the shade-avoidance syndrome in Arabidopsis by controlling elongation growth and flowering time. Plant Physiol. 1999; 119(3):909-15. PMC: 32105. DOI: 10.1104/pp.119.3.909. View

Zagotta M, Hicks K, Jacobs C, Young J, Hangarter R, Meeks-Wagner D . The Arabidopsis ELF3 gene regulates vegetative photomorphogenesis and the photoperiodic induction of flowering. Plant J. 1996; 10(4):691-702. DOI: 10.1046/j.1365-313x.1996.10040691.x. View

Fahlgren N, Feldman M, Gehan M, Wilson M, Shyu C, Bryant D . A Versatile Phenotyping System and Analytics Platform Reveals Diverse Temporal Responses to Water Availability in Setaria. Mol Plant. 2015; 8(10):1520-35. DOI: 10.1016/j.molp.2015.06.005. View

Strayer C, Oyama T, Schultz T, Raman R, Somers D, Mas P . Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog. Science. 2000; 289(5480):768-71. DOI: 10.1126/science.289.5480.768. View

Park M, Kwon Y, Gil K, Park C . LATE ELONGATED HYPOCOTYL regulates photoperiodic flowering via the circadian clock in Arabidopsis. BMC Plant Biol. 2016; 16(1):114. PMC: 4875590. DOI: 10.1186/s12870-016-0810-8. View

10.

Faure S, Turner A, Gruszka D, Christodoulou V, Davis S, von Korff M . Mutation at the circadian clock gene EARLY MATURITY 8 adapts domesticated barley (Hordeum vulgare) to short growing seasons. Proc Natl Acad Sci U S A. 2012; 109(21):8328-33. PMC: 3361427. DOI: 10.1073/pnas.1120496109. View

11.

Wang X, Jiang B, Gu L, Chen Y, Mora M, Zhu M . A photoregulatory mechanism of the circadian clock in Arabidopsis. Nat Plants. 2021; 7(10):1397-1408. DOI: 10.1038/s41477-021-01002-z. View

12.

Hsu P, Devisetty U, Harmer S . Accurate timekeeping is controlled by a cycling activator in Arabidopsis. Elife. 2013; 2:e00473. PMC: 3639509. DOI: 10.7554/eLife.00473. View

13.

Austen E, Weis A . What drives selection on flowering time? An experimental manipulation of the inherent correlation between genotype and environment. Evolution. 2015; 69(8):2018-33. DOI: 10.1111/evo.12709. View

14.

Niwa Y, Ito S, Nakamichi N, Mizoguchi T, Niinuma K, Yamashino T . Genetic linkages of the circadian clock-associated genes, TOC1, CCA1 and LHY, in the photoperiodic control of flowering time in Arabidopsis thaliana. Plant Cell Physiol. 2007; 48(7):925-37. DOI: 10.1093/pcp/pcm067. View

15.

Park Y, Kim J, Lee J, Lee B, Paek N, Park C . GIGANTEA Shapes the Photoperiodic Rhythms of Thermomorphogenic Growth in Arabidopsis. Mol Plant. 2020; 13(3):459-470. DOI: 10.1016/j.molp.2020.01.003. View

16.

Halliday K, Koornneef M, Whitelam G . Phytochrome B and at Least One Other Phytochrome Mediate the Accelerated Flowering Response of Arabidopsis thaliana L. to Low Red/Far-Red Ratio. Plant Physiol. 1994; 104(4):1311-1315. PMC: 159295. DOI: 10.1104/pp.104.4.1311. View

17.

Friedman J, Twyford A, Willis J, Blackman B . The extent and genetic basis of phenotypic divergence in life history traits in Mimulus guttatus. Mol Ecol. 2014; 24(1):111-22. PMC: 4657477. DOI: 10.1111/mec.13004. View

18.

Flis A, Sulpice R, Seaton D, Ivakov A, Liput M, Abel C . Photoperiod-dependent changes in the phase of core clock transcripts and global transcriptional outputs at dawn and dusk in Arabidopsis. Plant Cell Environ. 2016; 39(9):1955-81. DOI: 10.1111/pce.12754. View

19.

Picelli S, Faridani O, Bjorklund A, Winberg G, Sagasser S, Sandberg R . Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc. 2014; 9(1):171-81. DOI: 10.1038/nprot.2014.006. View

20.

Uhrig R, Schlapfer P, Roschitzki B, Hirsch-Hoffmann M, Gruissem W . Diurnal changes in concerted plant protein phosphorylation and acetylation in Arabidopsis organs and seedlings. Plant J. 2019; 99(1):176-194. DOI: 10.1111/tpj.14315. View