Bovine Oocyte Activation with Bull or Human Sperm by Conventional ICSI and Piezo-ICSI: Its Relationship with PLCɀ Activity

Overview

Journal Open Vet J

Specialty Veterinary Medicine

Date 2024 Jun 28

PMID 38938440

Authors

Gabriel Alvarez

Sofia Villanueva

Elizabeth Breininger

Marisa Geller

Claudio Ruhlmann

Gabriel Dalvit

Pablo Cetica

Masashige Kuwayama

Affiliations

Soon will be listed here.

Abstract

Background: The intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors.

Aim: Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with the phospholipase C zeta (PLCɀ) activity of both species.

Methods: matured bovine oocytes were randomly divided into five groups and were fertilized as follows: conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCɀ activity was determined in bull and human sperm samples.

Results: Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages and ICSI + chemical activation presented the highest 2 PN, cleavage, and blastocyst rates ( < 0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI ( < 0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm ( < 0.05). Higher values of PLCɀ activity were found in human sperm compared with bovine sperm ( < 0.05).

Conclusion: Our results suggest that the higher stability of the bovine sperm in combination with its relatively low content of PLCɀ impairs bovine oocyte activation after ICSI.

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