Circular RNA HSDL2 Promotes Breast Cancer Progression Via MiR-7978 ZNF704 Axis and Regulating Hippo Signaling Pathway

Overview

Journal Breast Cancer Res

Specialty Oncology

Date 2024 Jun 27

PMID 38937788

Authors

Dandan Wang

Sujin Yang

Mengmeng Lyu

Liping Xu

Shanliang Zhong

Dandan Yu

Affiliations

Soon will be listed here.

Abstract

Circular RNAs (circRNAs) are a new group of endogenous RNAs recently found to be involved in the development of various diseases, including their confirmed involvement in the progression of several types of cancers. Unluckily, the abnormal expression and functions of circRNAs in breast cancer shall be further investigated. This work aims to elucidate the action and molecular mechanism of circHSDL2 in the malignant progression of breast cancer. Differential expression profiles of circRNAs in breast cancer tissues relative to normal breast tissues and in the exosomes of breast cancer patients compared to healthy women were analyzed from databases to identify potentially functional circRNAs. CircHSDL2 was selected for further investigation. Cell proliferation, migration and invasion assays were done to assess the effect of circHSDL2 overexpression on breast cancer cells. Bioinformatics test and dual-luciferase reporter experiments were done to explore the interaction between circHSDL2 and miRNA. Downstream target genes were further investigated through proteomics analysis and Western blotting. The influence of circHSDL2 on breast cancer in vivo was evaluated through xenograft experiments in nude mice. Functional analysis demonstrated circHSDL2 overexpression promoted the division, movement, and invasion of breast cancer cells both in vivo and in vitro. Mechanistically, circHSDL2 acted as a sponge for miR-7978 to affect ZNF704 expression and thereby regulate the Hippo pathway in breast cancer cells. In conclusion, circHSDL2 regulates the Hippo pathway through the miR-7978/ZNF704 axis to facilitate the malignancy of breast cancer. This may be a potential biomarker and treatment target.

Citing Articles

Circ_0096710 facilitates tumor growth via controlling ADAM10 expression in esophageal squamous cell carcinoma.

Dong C, Li Z Thorac Cancer. 2024; 16(1):e15483.

PMID: 39620490 PMC: 11729450. DOI: 10.1111/1759-7714.15483.

References

Sang Y, Chen B, Song X, Li Y, Liang Y, Han D . circRNA_0025202 Regulates Tamoxifen Sensitivity and Tumor Progression via Regulating the miR-182-5p/FOXO3a Axis in Breast Cancer. Mol Ther. 2019; 27(9):1638-1652. PMC: 6731174. DOI: 10.1016/j.ymthe.2019.05.011. View

Yang S, Wang D, Zhou S, Zhang Q, Wang J, Zhong S . Identification of circRNA-miRNA networks for exploring an underlying prognosis strategy for breast cancer. Epigenomics. 2020; 12(2):101-125. DOI: 10.2217/epi-2019-0058. View

Qiu M, Xia W, Chen R, Wang S, Xu Y, Ma Z . The Circular RNA circPRKCI Promotes Tumor Growth in Lung Adenocarcinoma. Cancer Res. 2018; 78(11):2839-2851. DOI: 10.1158/0008-5472.CAN-17-2808. View

Li H, Xu J, Fang X, Zhu J, Yang J, Pan R . Circular RNA circRNA_000203 aggravates cardiac hypertrophy via suppressing miR-26b-5p and miR-140-3p binding to Gata4. Cardiovasc Res. 2019; 116(7):1323-1334. PMC: 7243276. DOI: 10.1093/cvr/cvz215. View

Sung H, Ferlay J, Siegel R, Laversanne M, Soerjomataram I, Jemal A . Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3):209-249. DOI: 10.3322/caac.21660. View

Chen B, Wei W, Huang X, Xie X, Kong Y, Dai D . circEPSTI1 as a Prognostic Marker and Mediator of Triple-Negative Breast Cancer Progression. Theranostics. 2018; 8(14):4003-4015. PMC: 6071524. DOI: 10.7150/thno.24106. View

Yu L, Gong X, Sun L, Zhou Q, Lu B, Zhu L . The Circular RNA Cdr1as Act as an Oncogene in Hepatocellular Carcinoma through Targeting miR-7 Expression. PLoS One. 2016; 11(7):e0158347. PMC: 4938625. DOI: 10.1371/journal.pone.0158347. View

Giaquinto A, Sung H, Miller K, Kramer J, Newman L, Minihan A . Breast Cancer Statistics, 2022. CA Cancer J Clin. 2022; 72(6):524-541. DOI: 10.3322/caac.21754. View

Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A . Circular RNAs are a large class of animal RNAs with regulatory potency. Nature. 2013; 495(7441):333-8. DOI: 10.1038/nature11928. View

10.

Kristensen L, Andersen M, Stagsted L, Ebbesen K, Hansen T, Kjems J . The biogenesis, biology and characterization of circular RNAs. Nat Rev Genet. 2019; 20(11):675-691. DOI: 10.1038/s41576-019-0158-7. View

11.

Caiment F, Gaj S, Claessen S, Kleinjans J . High-throughput data integration of RNA-miRNA-circRNA reveals novel insights into mechanisms of benzo[a]pyrene-induced carcinogenicity. Nucleic Acids Res. 2015; 43(5):2525-34. PMC: 4357716. DOI: 10.1093/nar/gkv115. View

12.

Salzman J, Chen R, Olsen M, Wang P, Brown P . Cell-type specific features of circular RNA expression. PLoS Genet. 2013; 9(9):e1003777. PMC: 3764148. DOI: 10.1371/journal.pgen.1003777. View

13.

Yang C, Wu J, Liu X, Wang Y, Liu B, Chen X . Circadian Rhythm Is Disrupted by ZNF704 in Breast Carcinogenesis. Cancer Res. 2020; 80(19):4114-4128. DOI: 10.1158/0008-5472.CAN-20-0493. View

14.

Rybak-Wolf A, Stottmeister C, Glazar P, Jens M, Pino N, Giusti S . Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed. Mol Cell. 2015; 58(5):870-85. DOI: 10.1016/j.molcel.2015.03.027. View

15.

Wang J, Zhang Q, Zhou S, Xu H, Wang D, Feng J . Circular RNA expression in exosomes derived from breast cancer cells and patients. Epigenomics. 2019; 11(4):411-421. DOI: 10.2217/epi-2018-0111. View

16.

Granados-Riveron J, Aquino-Jarquin G . The complexity of the translation ability of circRNAs. Biochim Biophys Acta. 2016; 1859(10):1245-51. DOI: 10.1016/j.bbagrm.2016.07.009. View

17.

Liu Q, Zhang X, Hu X, Dai L, Fu X, Zhang J . Circular RNA Related to the Chondrocyte ECM Regulates MMP13 Expression by Functioning as a MiR-136 'Sponge' in Human Cartilage Degradation. Sci Rep. 2016; 6:22572. PMC: 4773870. DOI: 10.1038/srep22572. View

18.

Zhang P, Gao C, Huang X, Lu J, Guo X, Shi G . Cancer cell-derived exosomal circUHRF1 induces natural killer cell exhaustion and may cause resistance to anti-PD1 therapy in hepatocellular carcinoma. Mol Cancer. 2020; 19(1):110. PMC: 7320583. DOI: 10.1186/s12943-020-01222-5. View

19.

Zhao W, Cui Y, Liu L, Qi X, Liu J, Ma S . Splicing factor derived circular RNA circUHRF1 accelerates oral squamous cell carcinoma tumorigenesis via feedback loop. Cell Death Differ. 2019; 27(3):919-933. PMC: 7206121. DOI: 10.1038/s41418-019-0423-5. View

20.

Wan L, Zhang L, Fan K, Cheng Z, Sun Q, Wang J . Circular RNA-ITCH Suppresses Lung Cancer Proliferation via Inhibiting the Wnt/β-Catenin Pathway. Biomed Res Int. 2016; 2016:1579490. PMC: 5013215. DOI: 10.1155/2016/1579490. View