Adjuvant Effects of a CC Chemokine for Enhancing the Efficacy of an Inactivated Vaccine in Nile Tilapia ()

Overview

Journal Vaccines (Basel)

Date 2024 Jun 27

PMID 38932370

Authors

Chayanit Soontara

Anurak Uchuwittayakul

Pattanapon Kayansamruaj

Piti Amparyup

Ratree Wongpanya

Prapansak Srisapoome

Affiliations

Soon will be listed here.

Abstract

In this study, the ability of a CC chemokine (-CC1) adjuvant to enhance the efficacy of a formalin-killed vaccine (WC) in inducing immune responses against in Nile tilapia was investigated through immune-related gene expression analysis, enzyme-linked immunosorbent assay (ELISA), transcriptome sequencing, and challenge tests. Significantly higher -specific IgM levels were detected in fish in the WC+CC group than in the WC alone or control groups at 8 days postvaccination (dpv). The WC vaccine group exhibited increased specific IgM levels at 15 dpv, comparable to those of the WC+CC group, with sustained higher levels observed in the latter group at 29 dpv and after challenge with for 14 days. Immune-related gene expression analysis revealed upregulation of all target genes in the control group compared to those in the vaccinated groups, with notable differences between the WC and WC+CC groups at various time intervals. Additionally, transcriptome analysis revealed differential gene expression profiles between the vaccinated (24 and 96 hpv) and control groups, with notable upregulation of immune-related genes in the vaccinated fish. Differential gene expression (DGE) analysis revealed significant upregulation of immunoglobulin and other immune-related genes in the control group compared to those in the vaccinated groups (24 and 96 hpv), with distinct patterns observed between the WC and WC+CC vaccine groups. Finally, challenge with a virulent strain of resulted in significantly higher survival rates for fish in the WC and WC+CC groups compared to fish in the control group, with a notable increase in survival observed in fish in the WC+CC group.

References

Sudhagar A, Kumar G, El-Matbouli M . Transcriptome Analysis Based on RNA-Seq in Understanding Pathogenic Mechanisms of Diseases and the Immune System of Fish: A Comprehensive Review. Int J Mol Sci. 2018; 19(1). PMC: 5796193. DOI: 10.3390/ijms19010245. View

Zhang J, Zhang S, Sun X, Xu X . Comparative transcriptome analysis reveals the immune response of turbot (Scophthalmus maximus) induced by inactivated bivalent vaccine. Fish Shellfish Immunol. 2022; 132:108461. DOI: 10.1016/j.fsi.2022.108461. View

Mohan T, Zhu W, Wang Y, Wang B . Applications of chemokines as adjuvants for vaccine immunotherapy. Immunobiology. 2017; 223(6-7):477-485. PMC: 5894355. DOI: 10.1016/j.imbio.2017.12.001. View

Huo X, Fan C, Ai T, Su J . The Combination of Molecular Adjuvant CCL35.2 and DNA Vaccine Significantly Enhances the Immune Protection of against CyHV-2 Infection. Vaccines (Basel). 2020; 8(4). PMC: 7712643. DOI: 10.3390/vaccines8040567. View

Su H, Yakovlev I, van Eerde A, Su J, Clarke J . Plant-Produced Vaccines: Future Applications in Aquaculture. Front Plant Sci. 2021; 12:718775. PMC: 8397579. DOI: 10.3389/fpls.2021.718775. View

Aldon Y, Kratochvil S, Shattock R, McKay P . Chemokine-Adjuvanted Plasmid DNA Induces Homing of Antigen-Specific and Non-Antigen-Specific B and T Cells to the Intestinal and Genital Mucosae. J Immunol. 2020; 204(4):903-913. PMC: 6994839. DOI: 10.4049/jimmunol.1901184. View

Geneva-Popova M, Popova-Belova S, Gardzheva P, Kraev K . A Study of IFN-α-Induced Chemokines CCL2, CXCL10 and CCL19 in Patients with Systemic Lupus Erythematosu. Life (Basel). 2022; 12(2). PMC: 8880517. DOI: 10.3390/life12020251. View

Losana G, Bovolenta C, Rigamonti L, Borghi I, Altare F, Jouanguy E . IFN-gamma and IL-12 differentially regulate CC-chemokine secretion and CCR5 expression in human T lymphocytes. J Leukoc Biol. 2002; 72(4):735-42. View

Mortazavi A, Williams B, McCue K, Schaeffer L, Wold B . Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008; 5(7):621-8. DOI: 10.1038/nmeth.1226. View

10.

Montecucco F, Steffens S, Burger F, Da Costa A, Bianchi G, Bertolotto M . Tumor necrosis factor-alpha (TNF-alpha) induces integrin CD11b/CD18 (Mac-1) up-regulation and migration to the CC chemokine CCL3 (MIP-1alpha) on human neutrophils through defined signalling pathways. Cell Signal. 2008; 20(3):557-68. DOI: 10.1016/j.cellsig.2007.11.008. View

11.

Clark-Lewis I, DEWALD B, Geiser T, Moser B, Baggiolini M . Platelet factor 4 binds to interleukin 8 receptors and activates neutrophils when its N terminus is modified with Glu-Leu-Arg. Proc Natl Acad Sci U S A. 1993; 90(8):3574-7. PMC: 46343. DOI: 10.1073/pnas.90.8.3574. View

12.

Maghazachi A, Schall T . CC chemokines induce the generation of killer cells from CD56+ cells. Eur J Immunol. 1996; 26(2):315-9. DOI: 10.1002/eji.1830260207. View

13.

Zhu J, Fu Q, Ao Q, Tan Y, Luo Y, Jiang H . Transcriptomic profiling analysis of tilapia (Oreochromis niloticus) following Streptococcus agalactiae challenge. Fish Shellfish Immunol. 2017; 62:202-212. DOI: 10.1016/j.fsi.2017.01.023. View

14.

Phan-Aram P, Mahasri G, Kayansamruaj P, Amparyup P, Srisapoome P . Immune Regulation, but Not Antibacterial Activity, Is a Crucial Function of Hepcidins in Resistance against Pathogenic Bacteria in Nile Tilapia ( Linn.). Biomolecules. 2020; 10(8). PMC: 7464455. DOI: 10.3390/biom10081132. View

15.

Monir M, Yusoff M, Zamri-Saad M, Azmai Amal M, Mohamad A, Azzam-Sayuti M . Effect of an Oral Bivalent Vaccine on Immune Response and Immune Gene Profiling in Vaccinated Red Tilapia ( spp.) during Infections with and . Biology (Basel). 2022; 11(9). PMC: 9495387. DOI: 10.3390/biology11091268. View

16.

Wu X, Xing J, Tang X, Sheng X, Chi H, Zhan W . Splenic protection network revealed by transcriptome analysis in inactivated vaccine-immunized flounder () against infection. Front Immunol. 2022; 13:1058599. PMC: 9681833. DOI: 10.3389/fimmu.2022.1058599. View

17.

Ma J, Bruce T, Jones E, Cain K . A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches. Microorganisms. 2019; 7(11). PMC: 6920890. DOI: 10.3390/microorganisms7110569. View

18.

Meachasompop P, Bunnoy A, Keaswejjareansuk W, Dechbumroong P, Namdee K, Srisapoome P . Development of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass () Nursery Farms. Vaccines (Basel). 2024; 12(1). PMC: 10818643. DOI: 10.3390/vaccines12010017. View

19.

Yang C, Yang C, Hsu W, Hsiao L, Tseng H, Shih Y . Tumor Necrosis Factor-α-Induced C-C Motif Chemokine Ligand 20 Expression through TNF Receptor 1-Dependent Activation of EGFR/p38 MAPK and JNK1/2/FoxO1 or the NF-κB Pathway in Human Cardiac Fibroblasts. Int J Mol Sci. 2022; 23(16). PMC: 9409325. DOI: 10.3390/ijms23169086. View

20.

Kumwan B, Bunnoy A, Chatchaiphan S, Kayansamruaj P, Dong H, Senapin S . First Investigation of the Optimal Timing of Vaccination of Nile Tilapia () Larvae against . Vaccines (Basel). 2023; 11(12). PMC: 10747866. DOI: 10.3390/vaccines11121753. View