Antiviral Activity of AgIO, a Unique Silver Compound

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2024 Jun 27

PMID 38932251

Authors

Mauri Erickson

Tyler J Boone

Patricia L Nadworny

Affiliations

Soon will be listed here.

Abstract

Pentasilver hexaoxoiodate (AgIO) has broad-spectrum antimicrobial efficacy, including the long-term prevention of microbial adherence, the rapid killing of planktonic microorganisms, and the elimination of mature biofilms. This study's goal was to determine whether it may also have antiviral activity against structurally distinct viruses. AgIO was tested following ASTM E1052-20, Standard Practice to Assess the Activity of Microbicides Against Viruses in Suspension, against adenovirus type 5, murine norovirus, poliovirus type 1, SARS-CoV-2 (original), and SARS-CoV-2 (omicron) (host cells: H1HeLa, RAW 264.7, LLC-MK2, Vero E6, and Vero E6, respectively). A 0.1 g/mL AgIO suspension was prepared and the viruses were exposed for 30 min, 4 h, or 24 h. Exposure to AgIO resulted in complete kill of SARS-CoV-2 (omicron) within 30 min, as well as complete kill of both SARS-CoV-2 (original) and the murine norovirus within 4 h. AgIO showed increasing activity over time against the adenovirus, but did not achieve a 3-log reduction within 24 h, and showed no antiviral activity against the poliovirus. These results demonstrate that AgIO has antiviral activity against medically important viruses, in addition to its well-characterized antimicrobial activity, suggesting that it may be valuable in situations where the prevention or simultaneous treatment of microbes and viruses are necessary.

References

Johnston H, Hutchison G, Christensen F, Peters S, Hankin S, Stone V . A review of the in vivo and in vitro toxicity of silver and gold particulates: particle attributes and biological mechanisms responsible for the observed toxicity. Crit Rev Toxicol. 2010; 40(4):328-46. DOI: 10.3109/10408440903453074. View

Nadworny P, Hickerson W, Holley-Harrison H, Bloom D, Grams T, Edwards T . Treatment of infection and inflammation associated with COVID-19, multi-drug resistant pneumonia and fungal sinusitis by nebulizing a nanosilver solution. Nanomedicine. 2023; 48:102654. PMC: 9839457. DOI: 10.1016/j.nano.2023.102654. View

Mathews S, Hans M, Mucklich F, Solioz M . Contact killing of bacteria on copper is suppressed if bacterial-metal contact is prevented and is induced on iron by copper ions. Appl Environ Microbiol. 2013; 79(8):2605-11. PMC: 3623184. DOI: 10.1128/AEM.03608-12. View

Gomes Von Borowski R, Silva Trentin D . Biofilms and Coronavirus Reservoirs: a Perspective Review. Appl Environ Microbiol. 2021; 87(18):e0085921. PMC: 8388801. DOI: 10.1128/AEM.00859-21. View

Nadworny P, Wang J, Tredget E, Burrell R . Anti-inflammatory activity of nanocrystalline silver in a porcine contact dermatitis model. Nanomedicine. 2008; 4(3):241-51. DOI: 10.1016/j.nano.2008.04.006. View

. Over-the-counter drug products containing colloidal silver ingredients or silver salts. Department of Health and Human Services (HHS), Public Health Service (PHS), Food and Drug Administration (FDA). Final rule. Fed Regist. 1999; 64(158):44653-8. View

Matthay M, Thompson B . Dexamethasone in hospitalised patients with COVID-19: addressing uncertainties. Lancet Respir Med. 2020; 8(12):1170-1172. PMC: 7598750. DOI: 10.1016/S2213-2600(20)30503-8. View

Garcia-Beltran W, Denis K, Hoelzemer A, Lam E, Nitido A, Sheehan M . mRNA-based COVID-19 vaccine boosters induce neutralizing immunity against SARS-CoV-2 Omicron variant. Cell. 2022; 185(3):457-466.e4. PMC: 8733787. DOI: 10.1016/j.cell.2021.12.033. View

Beigel J, Tomashek K, Dodd L, Mehta A, Zingman B, Kalil A . Remdesivir for the Treatment of Covid-19 - Final Report. N Engl J Med. 2020; 383(19):1813-1826. PMC: 7262788. DOI: 10.1056/NEJMoa2007764. View

10.

Huynh K, Chen K . Aggregation kinetics of citrate and polyvinylpyrrolidone coated silver nanoparticles in monovalent and divalent electrolyte solutions. Environ Sci Technol. 2011; 45(13):5564-71. PMC: 3137917. DOI: 10.1021/es200157h. View

11.

Incani V, Omar A, Prosperi-Porta G, Nadworny P . Ag5IO6: novel antibiofilm activity of a silver compound with application to medical devices. Int J Antimicrob Agents. 2015; 45(6):586-93. DOI: 10.1016/j.ijantimicag.2014.09.008. View

12.

Lin Q, Lim J, Xue K, Yew P, Owh C, Chee P . Sanitizing agents for virus inactivation and disinfection. View (Beijing). 2021; 1(2):e16. PMC: 7267133. DOI: 10.1002/viw2.16. View

13.

Wang Y, Quinsaat J, Li F, Isono T, Tajima K, Satoh T . Size Control and Enhanced Stability of Silver Nanoparticles by Cyclic Poly(ethylene glycol). Polymers (Basel). 2022; 14(21). PMC: 9657728. DOI: 10.3390/polym14214535. View

14.

Taylor P, Ussher A, Burrell R . Impact of heat on nanocrystalline silver dressings. Part I: Chemical and biological properties. Biomaterials. 2005; 26(35):7221-9. DOI: 10.1016/j.biomaterials.2005.05.040. View

15.

Thoulouze M, Alcover A . Can viruses form biofilms?. Trends Microbiol. 2011; 19(6):257-62. DOI: 10.1016/j.tim.2011.03.002. View

16.

Russell A, HUGO W . Antimicrobial activity and action of silver. Prog Med Chem. 1994; 31:351-70. DOI: 10.1016/s0079-6468(08)70024-9. View

17.

Gallant-Behm C, Yin H, Liu S, Heggers J, Langford R, Olson M . Comparison of in vitro disc diffusion and time kill-kinetic assays for the evaluation of antimicrobial wound dressing efficacy. Wound Repair Regen. 2005; 13(4):412-21. DOI: 10.1111/j.1067-1927.2005.130409.x. View

18.

Ershov V, Tarasova N, Abkhalimov E, Safonov A, Sorokin V, Ershov B . Photochemical Synthesis of Silver Hydrosol Stabilized by Carbonate Ions and Study of Its Bactericidal Impact on : Direct and Indirect Effects. Int J Mol Sci. 2022; 23(2). PMC: 8780126. DOI: 10.3390/ijms23020949. View

19.

Phillips P, Yang Q, Davis S, Sampson E, Azeke J, Hamad A . Antimicrobial dressing efficacy against mature Pseudomonas aeruginosa biofilm on porcine skin explants. Int Wound J. 2013; 12(4):469-83. PMC: 7950379. DOI: 10.1111/iwj.12142. View

20.

Naqvi S, Citardi M, Cattano D, Ostrosky-Zeichner L, Knackstedt M, Karni R . Povidone-iodine solution as SARS-CoV-2 prophylaxis for procedures of the upper aerodigestive tract a theoretical framework. J Otolaryngol Head Neck Surg. 2020; 49(1):77. PMC: 7590913. DOI: 10.1186/s40463-020-00474-x. View