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Nanoliposomes Permeability in a Microfluidic Drug Delivery Platform Across a 3D Hydrogel

Overview
Journal Pharmaceutics
Publisher MDPI
Date 2024 Jun 27
PMID 38931887
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Abstract

Nanoliposomes are nano-sized vesicles that can be used as drug delivery carriers with the ability to encapsulate both hydrophobic and hydrophilic compounds. Moreover, their lipid compositions facilitate their internalization by cells. However, the interaction between nanoliposomes and the membrane barrier of the human body is not well-known. If cellular tests and animal testing offer a solution, their lack of physiological relevance and ethical concerns make them unsuitable to properly mimic human body complexity. Microfluidics, which allows the environment of the human body to be imitated in a controlled way, can fulfil this role. However, existing models are missing the presence of something that would mimic a basal membrane, often consisting of a simple cell layer on a polymer membrane. In this study, we investigated the diffusion of nanoliposomes in a microfluidic system and found the optimal parameters to maximize their diffusion. Then, we incorporated a custom made GelMA with a controlled degree of substitution and studied the passage of fluorescently labeled nanoliposomes through this barrier. Our results show that highly substituted GelMA was more porous than lower substitution GelMA. Overall, our work lays the foundation for the incorporation of a hydrogel mimicking a basal membrane on a drug delivery microfluidic platform.

References
1.
Piazzini V, Landucci E, Graverini G, Pellegrini-Giampietro D, Bilia A, Bergonzi M . Stealth and Cationic Nanoliposomes as Drug Delivery Systems to Increase Andrographolide BBB Permeability. Pharmaceutics. 2018; 10(3). PMC: 6161272. DOI: 10.3390/pharmaceutics10030128. View

2.
Chen S, Wang Y, Lai J, Tan S, Wang M . Structure and Properties of Gelatin Methacryloyl (GelMA) Synthesized in Different Reaction Systems. Biomacromolecules. 2023; 24(6):2928-2941. DOI: 10.1021/acs.biomac.3c00302. View

3.
Young A, White O, Daniele M . Rheological Properties of Coordinated Physical Gelation and Chemical Crosslinking in Gelatin Methacryloyl (GelMA) Hydrogels. Macromol Biosci. 2020; 20(12):e2000183. PMC: 7738368. DOI: 10.1002/mabi.202000183. View

4.
Cameron A, Zeng B, Liu Y, Wang H, Soheilmoghaddam F, Cooper-White J . Biophysical properties of hydrogels for mimicking tumor extracellular matrix. Biomater Adv. 2022; 136:212782. DOI: 10.1016/j.bioadv.2022.212782. View

5.
Etheridge M, Campbell S, Erdman A, Haynes C, Wolf S, McCullough J . The big picture on nanomedicine: the state of investigational and approved nanomedicine products. Nanomedicine. 2012; 9(1):1-14. PMC: 4467093. DOI: 10.1016/j.nano.2012.05.013. View