Amoebicidal Effect of COVID Box Molecules Against : A Study of Cell Death

Overview

Journal Pharmaceuticals (Basel)

Publisher MDPI

Specialty Chemistry

Date 2024 Jun 27

PMID 38931475

Authors

Ines Sifaoui

Ruben L Rodriguez-Exposito

Maria Reyes-Batlle

Robert Sutak

Jose E Pinero

Jacob Lorenzo-Morales

Affiliations

Soon will be listed here.

Abstract

spp. can cause a sight threatening disease. At present, the current treatments used to treat spp. Infections, such as biguanide-based antimicrobials, remain inefficacious, with the appearance of resistant forms and high cytotoxicity to host cells. In this study, an initial screening was conducted against Neff and murine macrophages J774A.1 using alamarBlue™. Among the 160 compounds included in the cited box, 90% exhibited an inhibition of the parasite above 80%, while only 18.75% of the compounds inhibited the parasite with a lethality towards murine macrophage lower than 20%. Based on the amoebicidal activity, the cytotoxicity assay, and availability, Terconazole was chosen for the elucidation of the action mode in two clinical strains, and L10. A fluorescence image-based system and proteomic techniques were used to investigate the effect of the present azole on the cytoskeleton network and various programmed cell death features, including chromatin condensation and mitochondria dysfunction. Taking all the results together, we can suggest that Terconazole can induce programmed cell death (PCD) via the inhibition of sterol biosynthesis inhibition.

Citing Articles

Repurposing COVID-19 Compounds (via MMV COVID Box): Almitrine and Bortezomib Induce Programmed Cell Death in .

Bethencourt-Estrella C, Lopez-Arencibia A, Lorenzo-Morales J, Pinero J Pathogens. 2025; 14(2).

PMID: 40005505 PMC: 11858128. DOI: 10.3390/pathogens14020127.

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