Effects of Pea () Prebiotics on Intestinal Iron-Related Proteins and Microbial Populations In Vivo ()

Overview

Journal Nutrients

Date 2024 Jun 27

PMID 38931211

Authors

Abigail Armah

Cydney Jackson

Nikolai Kolba

Peter R Gracey

Viral Shukla

Olga I Padilla-Zakour

Tom Warkentin

Elad Tako

Affiliations

Soon will be listed here.

Abstract

Iron deficiency remains a public health challenge globally. Prebiotics have the potential to improve iron bioavailability by modulating intestinal bacterial population, increasing SCFA production, and stimulating expression of brush border membrane (BBM) iron transport proteins among iron-deficient populations. This study intended to investigate the potential effects of soluble extracts from the cotyledon and seed coat of three pea () varieties (CDC Striker, CDC Dakota, and CDC Meadow) on the expression of BBM iron-related proteins (DCYTB and DMT1) and populations of beneficial intestinal bacteria in vivo using the model by oral gavage (one day old chicks) with 1 mL of 50 mg/mL pea soluble extract solutions. The seed coat treatment groups increased the relative abundance of compared to the cotyledon treatment groups, with CDC Dakota seed coat (dark brown pigmented) recording the highest relative abundance of . In contrast, CDC Striker Cotyledon (dark-green-pigmented) significantly increased the relative abundance of ( < 0.05). Subsequently, the two dark-pigmented treatment groups (CDC Striker Cotyledon and CDC Dakota seed coats) recorded the highest expression of DCYTB. Our study suggests that soluble extracts from the pea seed coat and dark-pigmented pea cotyledon may improve iron bioavailability by affecting intestinal bacterial populations.

References

Hart J, Tako E, Kochian L, Glahn R . Identification of Black Bean (Phaseolus vulgaris L.) Polyphenols That Inhibit and Promote Iron Uptake by Caco-2 Cells. J Agric Food Chem. 2015; 63(25):5950-6. DOI: 10.1021/acs.jafc.5b00531. View

Guillon F, Champ M . Carbohydrate fractions of legumes: uses in human nutrition and potential for health. Br J Nutr. 2002; 88 Suppl 3:S293-306. DOI: 10.1079/BJN2002720. View

Noy Y, Sklan D . Yolk and exogenous feed utilization in the posthatch chick. Poult Sci. 2001; 80(10):1490-5. DOI: 10.1093/ps/80.10.1490. View

Kobayashi Y, Ohbuchi T, Fukuda T, Wakasugi E, Yasui R, Hamada M . Acidic xylooligosaccharide preserves hepatic iron storage level in adult female rats fed a low-iron diet. J Nutr Sci Vitaminol (Tokyo). 2011; 57(4):292-7. DOI: 10.3177/jnsv.57.292. View

Stuivenberg G, Burton J, Bron P, Reid G . Why Are Bifidobacteria Important for Infants?. Microorganisms. 2022; 10(2). PMC: 8880231. DOI: 10.3390/microorganisms10020278. View

Ghosal J, Bal M, Ranjit M, Das A, Behera M, Satpathy S . To what extent classic socio-economic determinants explain trends of anaemia in tribal and non-tribal women of reproductive age in India? Findings from four National Family Heath Surveys (1998-2021). BMC Public Health. 2023; 23(1):856. PMC: 10172723. DOI: 10.1186/s12889-023-15838-x. View

Jha A, Warkentin T . Biofortification of Pulse Crops: Status and Future Perspectives. Plants (Basel). 2020; 9(1). PMC: 7020478. DOI: 10.3390/plants9010073. View

Scott S, Chen-Edinboro L, Caulfield L, Murray-Kolb L . The impact of anemia on child mortality: an updated review. Nutrients. 2014; 6(12):5915-32. PMC: 4277007. DOI: 10.3390/nu6125915. View

Zhu X, Zhong T, Pandya Y, Joerger R . 16S rRNA-based analysis of microbiota from the cecum of broiler chickens. Appl Environ Microbiol. 2002; 68(1):124-37. PMC: 126585. DOI: 10.1128/AEM.68.1.124-137.2002. View

10.

Monnier L, Colette C, Aguirre L, MIROUZE J . Evidence and mechanism for pectin-reduced intestinal inorganic iron absorption in idiopathic hemochromatosis. Am J Clin Nutr. 1980; 33(6):1225-32. DOI: 10.1093/ajcn/33.6.1225. View

11.

Reider S, Moosmang S, Tragust J, Trgovec-Greif L, Tragust S, Perschy L . Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota-Results from the PAGODA Trial. Nutrients. 2020; 12(5). PMC: 7281958. DOI: 10.3390/nu12051257. View

12.

Lobreaux S, Briat J . Ferritin accumulation and degradation in different organs of pea (Pisum sativum) during development. Biochem J. 1991; 274 ( Pt 2):601-6. PMC: 1150181. DOI: 10.1042/bj2740601. View

13.

Chen H, Xu Y, Chen H, Liu H, Yu Q, Han L . Isolation and Identification of Polyphenols From Fresh Sweet Sorghum Stems and Their Antibacterial Mechanism Against Foodborne Pathogens. Front Bioeng Biotechnol. 2022; 9:770726. PMC: 8878683. DOI: 10.3389/fbioe.2021.770726. View

14.

Healey G, Murphy R, Butts C, Brough L, Whelan K, Coad J . Habitual dietary fibre intake influences gut microbiota response to an inulin-type fructan prebiotic: a randomised, double-blind, placebo-controlled, cross-over, human intervention study. Br J Nutr. 2018; 119(2):176-189. DOI: 10.1017/S0007114517003440. View

15.

Reed S, Neuman H, Glahn R, Koren O, Tako E . Characterizing the gut (Gallus gallus) microbiota following the consumption of an iron biofortified Rwandan cream seeded carioca (Phaseolus Vulgaris L.) bean-based diet. PLoS One. 2017; 12(8):e0182431. PMC: 5552115. DOI: 10.1371/journal.pone.0182431. View

16.

Bergamaschi G, Di Sabatino A, Pasini A, Ubezio C, Costanzo F, Grataroli D . Intestinal expression of genes implicated in iron absorption and their regulation by hepcidin. Clin Nutr. 2016; 36(5):1427-1433. DOI: 10.1016/j.clnu.2016.09.021. View

17.

Piskin E, Cianciosi D, Gulec S, Tomas M, Capanoglu E . Iron Absorption: Factors, Limitations, and Improvement Methods. ACS Omega. 2022; 7(24):20441-20456. PMC: 9219084. DOI: 10.1021/acsomega.2c01833. View

18.

Zhuo Z, Fang S, Hu Q, Huang D, Feng J . Digital gene expression profiling analysis of duodenum transcriptomes in SD rats administered ferrous sulfate or ferrous glycine chelate by gavage. Sci Rep. 2016; 6:37923. PMC: 5128800. DOI: 10.1038/srep37923. View

19.

Alexander C, Swanson K, Fahey G, Garleb K . Perspective: Physiologic Importance of Short-Chain Fatty Acids from Nondigestible Carbohydrate Fermentation. Adv Nutr. 2019; 10(4):576-589. PMC: 6628845. DOI: 10.1093/advances/nmz004. View

20.

Hall L, Robinson S, Buchanan-Smith H . Refining dosing by oral gavage in the dog: a protocol to harmonise welfare. J Pharmacol Toxicol Methods. 2015; 72:35-46. PMC: 4407634. DOI: 10.1016/j.vascn.2014.12.007. View