Integrated Metabolomics and Transcriptomics Analysis Reveals New Insights into Triterpene Biosynthesis in

Overview

Journal Plants (Basel)

Date 2024 Jun 27

PMID 38931033

Authors

Guo Wei

Yang Xu

Pengqing Wang

Hammad Hussain

Yudie Chen

Yuqing Shi

Kaikai Zhu

Mengjuan Bai

Yong Xu

Jianwen Wang

Liguo Feng

Affiliations

Soon will be listed here.

Abstract

is highly regarded for its aesthetic and therapeutic qualities. In particular, 's flowers are known to produce essential oils containing a mixture of volatile terpenes, phenylpropanoids, and other compounds. Despite this, extensive research exists on volatile terpenes in flowers, while the knowledge of non-volatile terpenes in distinct tissues is still limited. Using UPLC-ESI-MS/MS, a comprehensive analysis of the terpene metabolites in five different tissues of was conducted. These metabolites accumulated in distinct tissues, and the majority of them were triterpenoids. Transcriptome data were collected from five tissues using RNA-seq. Transcriptomics and metabolomics were utilized to evaluate the triterpene biosynthesis pathway, resulting in new insights into its regulation and biosynthesis. The was identified as a key enzyme in converting 2,3-oxidosqualene into α-amyrin, potentially contributing to the triterpene biosynthesis pathway. Furthermore, the expression of the gene was upregulated by salinity for 0.5 h and 1 h, with subsequent downregulation at 2 h. This study lays a foundation for future research on the biosynthesis and accumulation of triterpenes in .

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