A Sweet Potato MYB Transcription Factor Enhances Tolerance to Drought and Salt Stress in Transgenic Tobacco

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2024 Jun 27

PMID 38927629

Authors

Chong Wang

Jian Lei

Xiaojie Jin

Shasha Chai

Chunhai Jiao

Xinsun Yang

Lianjun Wang

Affiliations

Soon will be listed here.

Abstract

MYB transcription factors (TFs) play vital roles in plant growth, development, and response to adversity. Although the MYB gene family has been studied in many plant species, there is still little known about the function of R2R3 MYB TFs in sweet potato in response to abiotic stresses. In this study, an R2R3 MYB gene, was isolated from sweet potato (). was ectopically expressed in tobacco and the functional characterization was performed by overexpression in transgenic plants. The IbMYB330 protein has a 268 amino acid sequence and contains two highly conserved MYB domains. The molecular weight and isoelectric point of IbMYB330 are 29.24 kD and 9.12, respectively. The expression of in sweet potato is tissue-specific, and levels in the root were significantly higher than that in the leaf and stem. It showed that the expression of was strongly induced by PEG-6000, NaCl, and HO. Ectopic expression of led to increased transcript levels of stress-related genes such as , , , and . Moreover, compared to the wild-type (WT), transgenic tobacco overexpression of enhanced the tolerance to drought and salt stress treatment as CAT activity, POD activity, proline content, and protein content in transgenic tobacco had increased, while MDA content had decreased. Taken together, our study demonstrated that plays a role in enhancing the resistance of sweet potato to stresses. These findings lay the groundwork for future research on the R2R3-MYB genes of sweet potato and indicates that may be a candidate gene for improving abiotic stress tolerance in crops.

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