Evaluation of Heterologous ARS Activity in S. Cerevisiae Using Cloned DNA from S. Pombe

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1985 May 24

PMID 3892482

Citations 31

Authors

K Maundrell

A P Wright

M Piper

S Shall

Affiliations

Soon will be listed here.

Abstract

Cloned segments of Schizosaccharomyces pombe genomic DNA were screened for ARS activity in the native host, S. pombe, using high frequency transformation, phenotypic instability and extrachromosomal maintenance of unrearranged plasmid sequences as criteria for ARS function. This analysis revealed 12 ARS elements in a total of 230 kb of chromosomal DNA, indicating an average frequency of one ARS every 19 kb of genomic DNA. We then used these clones to assess the reliability of the S. cerevisiae assay for detecting ARS elements in heterologous DNA. The results show that not only does the S. cerevisiae assay fail to detect a large proportion of true ARS elements but it also wrongly identifies a significant proportion of clones which did not display ARS activity in the native host. We would therefore recommend restraint when extrapolating from observed ARS function of heterologous DNA in S. cerevisiae to a presumed analogous role in the original host.

Citing Articles

Identification and reconstitution of the origin recognition complex from Schizosaccharomyces pombe.

Moon K, Kong D, Lee J, Raychaudhuri S, Hurwitz J Proc Natl Acad Sci U S A. 1999; 96(22):12367-72.

PMID: 10535928 PMC: 22923. DOI: 10.1073/pnas.96.22.12367.

Clustered adenine/thymine stretches are essential for function of a fission yeast replication origin.

Okuno Y, Satoh H, Sekiguchi M, Masukata H Mol Cell Biol. 1999; 19(10):6699-709.

PMID: 10490609 PMC: 84658. DOI: 10.1128/MCB.19.10.6699.

DNA sequence and functional analysis of homologous ARS elements of Saccharomyces cerevisiae and S. carlsbergensis.

Theis J, Yang C, Schaefer C, Newlon C Genetics. 1999; 152(3):943-52.

PMID: 10388814 PMC: 1460646. DOI: 10.1093/genetics/152.3.943.

Conservation of ARS elements and chromosomal DNA replication origins on chromosomes III of Saccharomyces cerevisiae and S. carlsbergensis.

Yang C, Theis J, Newlon C Genetics. 1999; 152(3):933-41.

PMID: 10388813 PMC: 1460674. DOI: 10.1093/genetics/152.3.933.

Short DNA fragments without sequence similarity are initiation sites for replication in the chromosome of the yeast Yarrowia lipolytica.

Vernis L, Chasles M, Pasero P, Lepingle A, Gaillardin C, Fournier P Mol Biol Cell. 1999; 10(3):757-69.

PMID: 10069816 PMC: 25200. DOI: 10.1091/mbc.10.3.757.

References

Huberman J, Riggs A . On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol. 1968; 32(2):327-41. DOI: 10.1016/0022-2836(68)90013-2. View

Clewell D, Helinski D . Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970; 9(22):4428-40. DOI: 10.1021/bi00824a026. View

Nurse P . Genetic control of cell size at cell division in yeast. Nature. 1975; 256(5518):547-51. DOI: 10.1038/256547a0. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Beggs J . Transformation of yeast by a replicating hybrid plasmid. Nature. 1978; 275(5676):104-9. DOI: 10.1038/275104a0. View

Hand R . Eucaryotic DNA: organization of the genome for replication. Cell. 1978; 15(2):317-25. DOI: 10.1016/0092-8674(78)90001-6. View

Subramanian K, Shenk T . Definition of the boundaries of the origin of DNA replication in simian virus 40. Nucleic Acids Res. 1978; 5(10):3635-42. PMC: 342700. DOI: 10.1093/nar/5.10.3635. View

Struhl K, Stinchcomb D, Scherer S, Davis R . High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A. 1979; 76(3):1035-9. PMC: 383183. DOI: 10.1073/pnas.76.3.1035. View

Beach D, Piper M, Shall S . Isolation of chromosomal origins of replication in yeast. Nature. 1980; 284(5752):185-7. DOI: 10.1038/284185a0. View

10.

Broach J, Hicks J . Replication and recombination functions associated with the yeast plasmid, 2 mu circle. Cell. 1980; 21(2):501-8. DOI: 10.1016/0092-8674(80)90487-0. View

11.

Crews S, Ojala D, Posakony J, Nishiguchi J, Attardi G . Nucleotide sequence of a region of human mitochondrial DNA containing the precisely identified origin of replication. Nature. 1979; 277(5693):192-8. DOI: 10.1038/277192a0. View

12.

Stinchcomb D, Thomas M, Kelly J, Selker E, Davis R . Eukaryotic DNA segments capable of autonomous replication in yeast. Proc Natl Acad Sci U S A. 1980; 77(8):4559-63. PMC: 349883. DOI: 10.1073/pnas.77.8.4559. View

13.

Chan C, Tye B . Autonomously replicating sequences in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1980; 77(11):6329-33. PMC: 350277. DOI: 10.1073/pnas.77.11.6329. View

14.

Roberts T, Swanberg S, Poteete A, Riedel G, Backman K . A plasmid cloning vehicle allowing a positive selection for inserted fragments. Gene. 1980; 12(1-2):123-7. DOI: 10.1016/0378-1119(80)90022-0. View

15.

Zakian V . Origin of replication from Xenopus laevis mitochondrial DNA promotes high-frequency transformation of yeast. Proc Natl Acad Sci U S A. 1981; 78(5):3128-32. PMC: 319513. DOI: 10.1073/pnas.78.5.3128. View

16.

Gorman J, Dove W, Warren N . Isolation of Physarum DNA segments that support autonomous replication in yeast. Mol Gen Genet. 1981; 183(2):306-13. DOI: 10.1007/BF00270633. View

17.

Celniker S, Campbell J . Yeast DNA replication in vitro: initiation and elongation events mimic in vivo processes. Cell. 1982; 31(1):201-13. DOI: 10.1016/0092-8674(82)90420-2. View

18.

FANGMAN W, Hice R . ARS replication during the yeast S phase. Cell. 1983; 32(3):831-8. DOI: 10.1016/0092-8674(83)90069-7. View

19.

Henry 2nd B, Pagano J . Detection of autonomous replicating sequences (ars) in the genome of Epstein-Barr virus. Proc Natl Acad Sci U S A. 1983; 80(4):1096-100. PMC: 393535. DOI: 10.1073/pnas.80.4.1096. View

20.

Montiel J, Norbury C, Tuite M, Dobson M, MILLS J, Kingsman A . Characterization of human chromosomal DNA sequences which replicate autonomously in Saccharomyces cerevisiae. Nucleic Acids Res. 1984; 12(2):1049-68. PMC: 318555. DOI: 10.1093/nar/12.2.1049. View