Transcript Level of Telomerase Reverse-transcriptase (TERT) Gene in the Rainbow Trout (Oncorhynchus Mykiss) Eggs with Different Developmental Competence for Gynogenesis

Overview

Journal J Appl Genet

Publisher Springer

Specialty Genetics

Date 2024 Jun 26

PMID 38922511

Authors

Konrad Ocalewicz

Marcin Kucinski

Igor Jasielczuk

Artur Gurgul

Miroslaw Kucharski

Stefan Dobosz

Affiliations

Soon will be listed here.

Abstract

Expression of the telomerase reverse-transcriptase (TERT) gene and activity of telomerase have been reported in the somatic tissues and gonads in fish irrespective of their age and size. Nevertheless, little is known about TERT expression in the fish eggs. In the current study, the presence of the TERT transcripts was confirmed in the rainbow trout ovulated eggs before and after activation with nonirradiated and UV-irradiated (gynogenesis) sperm. Eggs originating from eight females had high and comparable quality expressed by similar hatching rates. However, survival of the gynogenetic larvae that hatched from eggs activated with UV-irradiated sperm and further exposed to the high hydrostatic pressure (HHP) shock for duplication of the maternal chromosomes varied between females from 2.1 ± 0.4 to 40.5 ± 2.2%. Increased level of TERT transcripts was observed in eggs originating from two females, and gametes from only one of them showed improved competence for gynogenesis (27.3 ± 1.9%). In turn, eggs from the female that exhibited the highest survival after gynogenetic activation were characterized by the lowest expression of the TERT gene. Telomerase in rainbow trout eggs may compensate erosion of the telomeres during early embryonic development; however, its upregulation does not assure better development after gynogenetic activation.

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