Boosting PRRSV-Specific Cellular Immunity: The Immunological Profiling of an Fc-Fused Multi-CTL Epitope Vaccine in Mice

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2024 Jun 26

PMID 38922021

Authors

Xinnuo Lei

Jinzhao Ban

Zhi Wu

Shinuo Cao

Mo Zhou

Li Zhang

Rui Zhu

Huipeng Lu

Shanyuan Zhu

Affiliations

Soon will be listed here.

Abstract

The continuously evolving PRRSV has been plaguing pig farms worldwide for over 30 years, with conventional vaccines suffering from insufficient protection and biosecurity risks. To address these challenges, we identified 10 PRRSV-specific CTL epitopes through enzyme-linked immunospot assay (ELISPOT) and constructed a multi-epitope peptide (PTE) by linking them in tandem. This PTE was then fused with a modified porcine Fc molecule to create the recombinant protein pFc-PTE. Our findings indicate that pFc-PTE effectively stimulates PRRSV-infected specific splenic lymphocytes to secrete high levels of interferon-gamma (IFN-γ) and is predicted to be non-toxic and non-allergenic. Compared to PTE alone, pFc-PTE not only induced a comparable cellular immune response in mice but also extended the duration of the immune response to at least 10 weeks post-immunization. Additionally, pFc-PTE predominantly induced a Th1 immune response, suggesting its potential advantage in enhancing cellular immunity. Consequently, pFc-PTE holds promise as a novel, safe, and potent candidate vaccine for PRRSV and may also provide new perspectives for vaccine design against other viral diseases.

Citing Articles

Current Status of Porcine Reproductive and Respiratory Syndrome Vaccines.

Wang H, Feng W Vaccines (Basel). 2025; 12(12.

PMID: 39772049 PMC: 11679953. DOI: 10.3390/vaccines12121387.

A Universal Multi-Epitope Vaccine Design Against Porcine Reproductive and Respiratory Syndrome Virus via Bioinformatics and Immunoinformatics Approaches.

Lei X, Wu Z, Feng Q, Jia W, Xie J, Zhou Q Vet Sci. 2024; 11(12).

PMID: 39728999 PMC: 11680090. DOI: 10.3390/vetsci11120659.

References

Gottlieb T, Ben-Yedidia T . Epitope-based approaches to a universal influenza vaccine. J Autoimmun. 2014; 54:15-20. DOI: 10.1016/j.jaut.2014.07.005. View

Wang C, Peng W, Kuo B, Ho Y, Wang M, Yang Y . Toward a pan-SARS-CoV-2 vaccine targeting conserved epitopes on spike and non-spike proteins for potent, broad and durable immune responses. PLoS Pathog. 2023; 19(4):e1010870. PMC: 10153712. DOI: 10.1371/journal.ppat.1010870. View

Zhao H, Wang F, Han X, Guo H, Liu C, Hou L . Recent advances in the study of NADC34-like porcine reproductive and respiratory syndrome virus in China. Front Microbiol. 2022; 13:950402. PMC: 9354828. DOI: 10.3389/fmicb.2022.950402. View

Liang C, Xia Q, Zhou J, Liu H, Chen Y, Liu Y . Identification of potential SLA-I-restricted CTL epitopes within the M protein of porcine reproductive and respiratory syndrome virus. Vet Microbiol. 2021; 259:109131. DOI: 10.1016/j.vetmic.2021.109131. View

Renukaradhya G, Meng X, Calvert J, Roof M, Lager K . Inactivated and subunit vaccines against porcine reproductive and respiratory syndrome: Current status and future direction. Vaccine. 2015; 33(27):3065-72. DOI: 10.1016/j.vaccine.2015.04.102. View

Liu X, Zhou X, Noor A, Zhang X, Song C, Sun H . Enhancing half-life and cytotoxicity of porcine respiratory and reproductive syndrome virus soluble receptors by taming their Fc domains. Vet Microbiol. 2022; 273:109526. DOI: 10.1016/j.vetmic.2022.109526. View

Wang J, Zhang M, Cui X, Gao X, Sun W, Ge X . Attenuated Porcine Reproductive and Respiratory Syndrome Virus Regains Its Fatal Virulence by Serial Passaging in Pigs or Porcine Alveolar Macrophages To Increase Its Adaptation to Target Cells. Microbiol Spectr. 2022; 10(6):e0308422. PMC: 9769833. DOI: 10.1128/spectrum.03084-22. View

McGee M, Huang W . Evolutionary conservation and positive selection of influenza A nucleoprotein CTL epitopes for universal vaccination. J Med Virol. 2022; 94(6):2578-2587. PMC: 9052727. DOI: 10.1002/jmv.27662. View

Buonaguro L, Tagliamonte M . Peptide-based vaccine for cancer therapies. Front Immunol. 2023; 14:1210044. PMC: 10467431. DOI: 10.3389/fimmu.2023.1210044. View

10.

Malonis R, Lai J, Vergnolle O . Peptide-Based Vaccines: Current Progress and Future Challenges. Chem Rev. 2019; 120(6):3210-3229. PMC: 7094793. DOI: 10.1021/acs.chemrev.9b00472. View

11.

Wu F, Peng K, Tian J, Xu X, Zhou E, Chen H . Immune response to Fc tagged GP5 glycoproteins of porcine reproductive and respiratory syndrome virus. Viral Immunol. 2014; 27(7):343-9. DOI: 10.1089/vim.2014.0041. View

12.

Renukaradhya G, Meng X, Calvert J, Roof M, Lager K . Live porcine reproductive and respiratory syndrome virus vaccines: Current status and future direction. Vaccine. 2015; 33(33):4069-80. DOI: 10.1016/j.vaccine.2015.06.092. View

13.

Liu P, Bai Y, Jiang X, Zhou L, Yuan S, Yao H . High reversion potential of a cell-adapted vaccine candidate against highly pathogenic porcine reproductive and respiratory syndrome. Vet Microbiol. 2018; 227:133-142. DOI: 10.1016/j.vetmic.2018.10.004. View

14.

Rath T, Baker K, Dumont J, Peters R, Jiang H, Qiao S . Fc-fusion proteins and FcRn: structural insights for longer-lasting and more effective therapeutics. Crit Rev Biotechnol. 2013; 35(2):235-54. PMC: 4876602. DOI: 10.3109/07388551.2013.834293. View

15.

Song J, Wang M, Zhou L, Tian P, Sun Z, Sun J . A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response. J Nanobiotechnology. 2023; 21(1):424. PMC: 10647103. DOI: 10.1186/s12951-023-02210-9. View

16.

Gong W, Pan C, Cheng P, Wang J, Zhao G, Wu X . Peptide-Based Vaccines for Tuberculosis. Front Immunol. 2022; 13:830497. PMC: 8841753. DOI: 10.3389/fimmu.2022.830497. View

17.

Larche M, Wraith D . Peptide-based therapeutic vaccines for allergic and autoimmune diseases. Nat Med. 2005; 11(4 Suppl):S69-76. DOI: 10.1038/nm1226. View

18.

Zhang L, Wang L, Cao S, Lv H, Huang J, Zhang G . Nanobody Nb6 fused with porcine IgG Fc as the delivering tag to inhibit porcine reproductive and respiratory syndrome virus replication in porcine alveolar macrophages. Vet Res. 2021; 52(1):25. PMC: 7887809. DOI: 10.1186/s13567-020-00868-9. View

19.

Vashisht K, Goldberg T, Husmann R, Schnitzlein W, Zuckermann F . Identification of immunodominant T-cell epitopes present in glycoprotein 5 of the North American genotype of porcine reproductive and respiratory syndrome virus. Vaccine. 2008; 26(36):4747-53. DOI: 10.1016/j.vaccine.2008.06.047. View

20.

Yue C, Wang P, Tian J, Gao G, Liu K, Liu W . Seeing the T cell Immunity of SARS-CoV-2 and SARS-CoV: Believing the Epitope-Oriented Vaccines. Int J Biol Sci. 2023; 19(13):4052-4060. PMC: 10496500. DOI: 10.7150/ijbs.80468. View