Photoreactivation Activities of Rad5, Rad16A and Rad16B Help to Recover from Solar Ultraviolet Damage

Overview

Journal J Fungi (Basel)

Date 2024 Jun 26

PMID 38921406

Authors

Xin-Cheng Luo

Lei Yu

Si-Yuan Xu

Sheng-Hua Ying

Ming-Guang Feng

Affiliations

Soon will be listed here.

Abstract

In budding yeast, Rad5 and Rad7-Rad16 play respective roles in the error-free post-replication repair and nucleotide excision repair of ultraviolet-induced DNA damage; however, their homologs have not yet been studied in non-yeast fungi. In the fungus , a deficiency in the Rad7 homolog, Rad5 ortholog and two Rad16 paralogs (Rad16A/B) instituted an ability to help the insect-pathogenic fungus to recover from solar UVB damage through photoreactivation. The fungal lifecycle-related phenotypes were not altered in the absence of , or while severe defects in growth and conidiation were caused by the double deletion of and . Compared with the wild-type and complemented strains, the mutants showed differentially reduced activities regarding the resilience of UVB-impaired conidia at 25 °C through a 12-h incubation in a regime of visible light plus dark (L/D 3:9 h or 5:7 h for photoreactivation) or of full darkness (dark reactivation) mimicking a natural nighttime. The estimates of the median lethal UVB dose LD from the dark and L/D treatments revealed greater activities of Rad5 and Rad16B than of Rad16A and additive activities of Rad16A and Rad16B in either NER-dependent dark reactivation or photorepair-dependent photoreactivation. However, their dark reactivation activities were limited to recovering low UVB dose-impaired conidia but were unable to recover conidia impaired by sublethal and lethal UVB doses as did their photoreactivation activities at L/D 3:9 or 5:7, unless the night/dark time was doubled or further prolonged. Therefore, the anti-UV effects of Rad5, Rad16A and Rad16B in depend primarily on photoreactivation and are mechanistically distinct from those for their yeast homologs.

References

Fernandes E, Rangel D, Moraes A, Bittencourt V, Roberts D . Variability in tolerance to UV-B radiation among Beauveria spp. isolates. J Invertebr Pathol. 2007; 96(3):237-43. DOI: 10.1016/j.jip.2007.05.007. View

Lafrance-Vanasse J, Arseneault G, Cappadocia L, Chen H, Legault P, Omichinski J . Structural and functional characterization of interactions involving the Tfb1 subunit of TFIIH and the NER factor Rad2. Nucleic Acids Res. 2012; 40(12):5739-50. PMC: 3384317. DOI: 10.1093/nar/gks194. View

Gillette T, Yu S, Zhou Z, Waters R, Johnston S, Reed S . Distinct functions of the ubiquitin-proteasome pathway influence nucleotide excision repair. EMBO J. 2006; 25(11):2529-38. PMC: 1478203. DOI: 10.1038/sj.emboj.7601120. View

Malik S, Chaurasia P, Lahudkar S, Durairaj G, Shukla A, Bhaumik S . Rad26p, a transcription-coupled repair factor, is recruited to the site of DNA lesion in an elongating RNA polymerase II-dependent manner in vivo. Nucleic Acids Res. 2009; 38(5):1461-77. PMC: 2836574. DOI: 10.1093/nar/gkp1147. View

Rodriguez K, Wang Z, Friedberg E, Tomkinson A . Identification of functional domains within the RAD1.RAD10 repair and recombination endonuclease of Saccharomyces cerevisiae. J Biol Chem. 1996; 271(34):20551-8. DOI: 10.1074/jbc.271.34.20551. View

Reed S, You Z, Friedberg E . The yeast RAD7 and RAD16 genes are required for postincision events during nucleotide excision repair. In vitro and in vivo studies with rad7 and rad16 mutants and purification of a Rad7/Rad16-containing protein complex. J Biol Chem. 1998; 273(45):29481-8. DOI: 10.1074/jbc.273.45.29481. View

Suter B, Wellinger R, Thoma F . DNA repair in a yeast origin of replication: contributions of photolyase and nucleotide excision repair. Nucleic Acids Res. 2000; 28(10):2060-8. PMC: 105381. DOI: 10.1093/nar/28.10.2060. View

Xu S, Yu L, Luo X, Ying S, Feng M . Co-Regulatory Roles of WC1 and WC2 in Asexual Development and Photoreactivation of . J Fungi (Basel). 2023; 9(3). PMC: 10056576. DOI: 10.3390/jof9030290. View

Berrocal-Tito G, Esquivel-Naranjo E, Horwitz B, Herrera-Estrella A . Trichoderma atroviride PHR1, a fungal photolyase responsible for DNA repair, autoregulates its own photoinduction. Eukaryot Cell. 2007; 6(9):1682-92. PMC: 2043357. DOI: 10.1128/EC.00208-06. View

10.

Griffiths H, Mistry P, Herbert K, Lunec J . Molecular and cellular effects of ultraviolet light-induced genotoxicity. Crit Rev Clin Lab Sci. 1998; 35(3):189-237. DOI: 10.1080/10408369891234192. View

11.

Gangavarapu V, Haracska L, Unk I, Johnson R, Prakash S, Prakash L . Mms2-Ubc13-dependent and -independent roles of Rad5 ubiquitin ligase in postreplication repair and translesion DNA synthesis in Saccharomyces cerevisiae. Mol Cell Biol. 2006; 26(20):7783-90. PMC: 1636848. DOI: 10.1128/MCB.01260-06. View

12.

Yu L, Xu S, Luo X, Ying S, Feng M . High photoreactivation activities of Rad2 and Rad14 in recovering insecticidal Beauveria bassiana from solar UV damage. J Photochem Photobiol B. 2024; 251:112849. DOI: 10.1016/j.jphotobiol.2024.112849. View

13.

Wang D, Fu B, Tong S, Ying S, Feng M . Two Photolyases Repair Distinct DNA Lesions and Reactivate UVB-Inactivated Conidia of an Insect Mycopathogen under Visible Light. Appl Environ Microbiol. 2018; 85(4). PMC: 6365834. DOI: 10.1128/AEM.02459-18. View

14.

Smerdon M, Thoma F . Site-specific DNA repair at the nucleosome level in a yeast minichromosome. Cell. 1990; 61(4):675-84. DOI: 10.1016/0092-8674(90)90479-x. View

15.

Sancar A . No "End of History" for photolyases. Science. 1996; 272(5258):48-9. DOI: 10.1126/science.272.5258.48. View

16.

Bailly V, Sommers C, Sung P, Prakash L, Prakash S . Specific complex formation between proteins encoded by the yeast DNA repair and recombination genes RAD1 and RAD10. Proc Natl Acad Sci U S A. 1992; 89(17):8273-7. PMC: 49900. DOI: 10.1073/pnas.89.17.8273. View

17.

Guzder S, Sung P, Prakash L, Prakash S . Synergistic interaction between yeast nucleotide excision repair factors NEF2 and NEF4 in the binding of ultraviolet-damaged DNA. J Biol Chem. 1999; 274(34):24257-62. DOI: 10.1074/jbc.274.34.24257. View

18.

Evans E, Moggs J, Hwang J, Egly J, Wood R . Mechanism of open complex and dual incision formation by human nucleotide excision repair factors. EMBO J. 1997; 16(21):6559-73. PMC: 1170260. DOI: 10.1093/emboj/16.21.6559. View

19.

Tong S, Feng M . Molecular basis and regulatory mechanisms underlying fungal insecticides' resistance to solar ultraviolet irradiation. Pest Manag Sci. 2021; 78(1):30-42. DOI: 10.1002/ps.6600. View

20.

Staresincic L, Fagbemi A, Enzlin J, Gourdin A, Wijgers N, Dunand-Sauthier I . Coordination of dual incision and repair synthesis in human nucleotide excision repair. EMBO J. 2009; 28(8):1111-20. PMC: 2683701. DOI: 10.1038/emboj.2009.49. View