Electrophysiological Insights with Brain Organoid Models: a Brief Review

Overview

Journal BMB Rep

Specialties Biochemistry
Molecular Biology

Date 2024 Jun 26

PMID 38919012

Authors

Rian Kang

Soomin Park

Saewoon Shin

Gyusoo Bak

Jong-Chan Park

Affiliations

Soon will be listed here.

Abstract

Brain organoid is a three-dimensional (3D) tissue derived from stem cells such as induced pluripotent stem cells (iPSCs) embryonic stem cells (ESCs) that reflect real human brain structure. It replicates the complexity and development of the human brain, enabling studies of the human brain in vitro. With emerging technologies, its application is various, including disease modeling and drug screening. A variety of experimental methods have been used to study structural and molecular characteristics of brain organoids. However, electrophysiological analysis is necessary to understand their functional characteristics and complexity. Although electrophysiological approaches have rapidly advanced for monolayered cells, there are some limitations in studying electrophysiological and neural network characteristics due to the lack of 3D characteristics. Herein, electrophysiological measurement and analytical methods related to neural complexity and 3D characteristics of brain organoids are reviewed. Overall, electrophysiological understanding of brain organoids allows us to overcome limitations of monolayer in vitro cell culture models, providing deep insights into the neural network complex of the real human brain and new ways of disease modeling. [BMB Reports 2024; 57(7): 311-317].

Citing Articles

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PMID: 40013084 PMC: 11850696. DOI: 10.1007/s43188-025-00279-y.

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