Leishmania Infantum Detection in Nyssomyia Neivai and Dogs in Southern Brazil

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2024 Jun 25

PMID 38918858

Authors

Sabrina Fernandes Cardoso

Joao Victor Costa Guesser

Andressa Alencastre Fuzari Rodrigues

Reginaldo Pecanha Brazil

Luisa Damazio Pitaluga Rona

Andre Nobrega Pitaluga

Affiliations

Soon will be listed here.

Abstract

Background: The sand fly Nyssomyia neivai is one of the most abundant species in Southern Brazil. It is frequently found in areas that are foci of visceral leishmaniasis in the state of Santa Catarina, caused by Leishmania infantum. In this region, the main vector of L. infantum, Lutzomyia longipalpis, has not been detected. In the absence of L. longipalpis, this study aimed to identify the sand fly fauna and diagnose any potential Leishmania spp. infection in sand flies and in dogs in a region of Southern Brazil that experienced a recent canine visceral leishmaniasis outbreak.

Methods: This report includes a survey of the sand fly fauna at the Zoonosis Control Center of the Municipality of Tubarão (Santa Catarina, Brazil). Molecular tests were conducted to investigate Leishmania spp. natural infection in sand flies using polymerase chain reaction (PCR). In positive females, in addition to morphological identification, molecular analysis through DNA barcoding was performed to determine the sand fly species. Additionally, the dogs were tested for the presence of Leishmania spp. using a non-invasive technique for the collection of biological material, to be assessed by PCR.

Results: A total of 3419 sand flies, belonging to five genera, were collected. Nyssomyia neivai was the most abundant species (85.8%), followed by Migonemyia migonei (13.3%), Pintomyia fischeri (0.8%), Evandromyia edwardsi (< 0.1%), and species of the genus Brumptomyia. (0.1%). Out of the 509 non-engorged females analyzed by PCR, two (0.4%) carried L. infantum DNA. The naturally infected females were identified as Ny. neivai, in both morphological and molecular analysis. In addition, two out of 47 conjunctival swabs from dogs tested positive for L. infantum, yielding an infection rate of 4.2%.

Conclusions: These results confirm the presence of Ny. neivai naturally infected with L. infantum in an area where dogs were also infected by the parasite, suggesting its potential role as a vector in Southern Brazil.

Citing Articles

Clinical, histopathological and parasitological follow-up of dogs naturally infected by Leishmania infantum before and after miltefosine treatment and associated therapies.

Rosar A, Martins C, Menin A, Reck C, Grisard E, Wagner G PLoS One. 2025; 20(1):e0313167.

PMID: 39787198 PMC: 11717219. DOI: 10.1371/journal.pone.0313167.

References

Geisweid K, Weber K, Sauter-Louis C, Hartmann K . Evaluation of a conjunctival swab polymerase chain reaction for the detection of Leishmania infantum in dogs in a non-endemic area. Vet J. 2013; 198(1):187-92. DOI: 10.1016/j.tvjl.2013.07.025. View

Galvis-Ovallos F, Ueta A, Marques G, Sarmento A, Araujo G, Sandoval C . Detection of Pintomyia fischeri (Diptera: Psychodidae) With Leishmania infantum (Trypanosomatida: Trypanosomatidae) Promastigotes in a Focus of Visceral Leishmaniasis in Brazil. J Med Entomol. 2020; 58(2):830-836. DOI: 10.1093/jme/tjaa199. View

McIntyre S, Rangel E, Ready P, Carvalho B . Species-specific ecological niche modelling predicts different range contractions for Lutzomyia intermedia and a related vector of Leishmania braziliensis following climate change in South America. Parasit Vectors. 2017; 10(1):157. PMC: 5366140. DOI: 10.1186/s13071-017-2093-9. View

Cecilio P, Cordeiro-da-Silva A, Oliveira F . Sand flies: Basic information on the vectors of leishmaniasis and their interactions with Leishmania parasites. Commun Biol. 2022; 5(1):305. PMC: 8979968. DOI: 10.1038/s42003-022-03240-z. View

Rodrigues A, Melo L, Magalhaes R, de Moraes N, de Souza Junior A, Bevilaqua C . Molecular identification of Lutzomyia migonei (Diptera: Psychodidae) as a potential vector for Leishmania infantum (Kinetoplastida: Trypanosomatidae). Vet Parasitol. 2016; 220:28-32. DOI: 10.1016/j.vetpar.2016.02.018. View

Vigoder F, Souza N, Brazil R, Bruno R, Costa P, Ritchie M . Phenotypic differentiation in love song traits among sibling species of the Lutzomyia longipalpis complex in Brazil. Parasit Vectors. 2015; 8:290. PMC: 4456791. DOI: 10.1186/s13071-015-0900-8. View

de Souza Leal Diniz M, Ovallos F, Gomes C, Lavitschka C, Galati E . Host-biting rate and susceptibility of some suspected vectors to Leishmania braziliensis. Parasit Vectors. 2014; 7:139. PMC: 3976554. DOI: 10.1186/1756-3305-7-139. View

Brisola Marcondes C, Bittencourt I, Stoco P, Eger I, Grisard E, Steindel M . Natural infection of Nyssomyia neivai (Pinto, 1926) (Diptera: Psychodidae, Phlebotominae) by Leishmania (Viannia) spp. in Brazil. Trans R Soc Trop Med Hyg. 2009; 103(11):1093-7. DOI: 10.1016/j.trstmh.2008.12.006. View

Saraiva L, Carvalho G, Gontijo C, Quaresma P, Lima A, Falcao A . Natural infection of Lutzomyia neivai and Lutzomyia sallesi (Diptera: Psychodidae) by Leishmania infantum chagasi in Brazil. J Med Entomol. 2009; 46(5):1159-63. DOI: 10.1603/033.046.0525. View

10.

Shimabukuro P, Andrade A, Galati E . Checklist of American sand flies (Diptera, Psychodidae, Phlebotominae): genera, species, and their distribution. Zookeys. 2017; (660):67-106. PMC: 5549530. DOI: 10.3897/zookeys.660.10508. View

11.

Moya S, Pech-May A, Quintana M, Manteca-Acosta M, Salomon O . Phylogenetic relationships of closely-related phlebotomine sand flies (Diptera: Psychodidae) of Nyssomyia genus and Lutzomyia subgenus. Mem Inst Oswaldo Cruz. 2020; 115:e200220. PMC: 7491277. DOI: 10.1590/0074-02760200220. View

12.

Souza N, Vigoder F, Araki A, Ward R, Kyriacou C, Peixoto A . Analysis of the copulatory courtship songs of Lutzomyia longipalpis in six populations from Brazil. J Med Entomol. 2004; 41(5):906-13. DOI: 10.1603/0022-2585-41.5.906. View

13.

Cardenas R, Sandoval C, Rodriguez-Morales A, Franco-Paredes C . Impact of climate variability in the occurrence of leishmaniasis in northeastern Colombia. Am J Trop Med Hyg. 2006; 75(2):273-7. View

14.

Marassa A, Galati E, Bergamaschi D, Consales C . Blood feeding patterns of Nyssomyia intermedia and Nyssomyia neivai (Diptera, Psychodidae) in a cutaneous leishmaniasis endemic area of the Ribeira Valley, State of São Paulo, Brazil. Rev Soc Bras Med Trop. 2013; 46(5):547-54. DOI: 10.1590/0037-8682-0168-2013. View

15.

Quiroga C, Cevallos V, Morales D, Baldeon M, Cardenas P, Rojas-Silva P . Molecular Identification of Leishmania spp. in Sand Flies (Diptera: Psychodidae, Phlebotominae) From Ecuador. J Med Entomol. 2017; 54(6):1704-1711. PMC: 5850347. DOI: 10.1093/jme/tjx122. View

16.

Morelli L, Pita-Pereira D, Britto C, de Araujo-Pereira T, de Souza L, Germano K . Leishmania (Leishmania) infantum DNA detection in Nyssomyia neivai in Vale do Ribeira, Paraná, Brazil. Mem Inst Oswaldo Cruz. 2024; 119:e230173. PMC: 10836809. DOI: 10.1590/0074-02760230173. View

17.

Da Silva A, de Camargo N, Dos Santos D, Massafera R, Ferreira A, Postai C . [Diversity, distribution and abundance of sandflies (Diptera: Psychodidae) in Parana State, Southern Brazil]. Neotrop Entomol. 2008; 37(2):209-25. DOI: 10.1590/s1519-566x2008000200017. View

18.

Galvis-Ovallos F, Dantas da Silva M, Bispo G, de Oliveira A, Neto J, Dos Santos Malafronte R . Canine visceral leishmaniasis in the metropolitan area of São Paulo: Pintomyia fischeri as potential vector of Leishmania infantum. Parasite. 2017; 24:2. PMC: 5780806. DOI: 10.1051/parasite/2017002. View

19.

Brisola Marcondes C, Conceicao M, Portes M, Simao B . Phlebotomine sandflies in a focus of dermal leishmaniasis in the eastern region of the Brazilian State of Santa Catarina: preliminary results (Diptera: Psychodidae). Rev Soc Bras Med Trop. 2005; 38(4):353-5. DOI: 10.1590/s0037-86822005000400016. View

20.

Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig H, Presber W . PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis. 2003; 47(1):349-58. DOI: 10.1016/s0732-8893(03)00093-2. View