HEPPAR1 and PIWIL2 As Panel Markers for Hepatocellular Carcinoma

Overview

Journal Asian Pac J Cancer Prev

Specialty Oncology

Date 2024 Jun 25

PMID 38918675

Authors

Gehan Hammad

Mona Magdy

Tarek Aboushousha

Amr Abdelraouf

Samah Mamdouh

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of this study was to evaluate the expression profiles of PIWI-like protein- 2 (PIWIL2), and HepPar1 and their immunohistochemical (IHC) characteristics in Hepatocellular Carcinoma (HCC), and determine their correlation with clinicopathological parameters of this type of cancer to determine their diagnostic value in combination.

Methods: Seventy-five patients with HCC were assessed for the expression of PIWIL2 in serum and tissue using real-time polymerase chain reaction (RT-PCR) and IHC was performed for PIWIL2 and HepPar1 was performed on all patients.

Results: A statistically significantly higher level of PIWIL2 was found in HCC compared to controls (p≤0.001). Both HepPar1 and PIWIL2 were detected in 84% of HCC cases, the diagnostic and prognostic factors for PIWIL2 were found to be significant in liver tumour tissue samples and non-tumorous sections p<0.001, and the same was observed for serum samples and results of healthy serum controls (p<0.001) when compared to AFP.

Conclusion: Our results affirm the hypothesis that reactivation of PIWI expression in various caner types is crucial for cancer development, and that a possible panel maybe used for these markers HCC diagnosis.

References

Balaratnam S, West N, Basu S . A piRNA utilizes HILI and HIWI2 mediated pathway to down-regulate ferritin heavy chain 1 mRNA in human somatic cells. Nucleic Acids Res. 2018; 46(20):10635-10648. PMC: 6237762. DOI: 10.1093/nar/gky728. View

Li W, Martinez-Useros J, Garcia-Carbonero N, Fernandez-Acenero M, Orta A, Ortega-Medina L . The Clinical Significance of PIWIL3 and PIWIL4 Expression in Pancreatic Cancer. J Clin Med. 2020; 9(5). PMC: 7287605. DOI: 10.3390/jcm9051252. View

Tan Y, Liu L, Liao M, Zhang C, Hu S, Zou M . Emerging roles for PIWI proteins in cancer. Acta Biochim Biophys Sin (Shanghai). 2015; 47(5):315-24. DOI: 10.1093/abbs/gmv018. View

Jepsen P, Ott P, Andersen P, Sorensen H, Vilstrup H . Risk for hepatocellular carcinoma in patients with alcoholic cirrhosis: a Danish nationwide cohort study. Ann Intern Med. 2012; 156(12):841-7, W295. DOI: 10.7326/0003-4819-156-12-201206190-00004. View

Cai A, Hu Y, Zhou Z, Qi Q, Wu Y, Dong P . PIWI-Interacting RNAs (piRNAs): Promising Applications as Emerging Biomarkers for Digestive System Cancer. Front Mol Biosci. 2022; 9:848105. PMC: 8829394. DOI: 10.3389/fmolb.2022.848105. View

Moudi B, Mahmoudzadeh-Sagheb H, Heidari Z . Hepatocyte paraffin 1 and arginase-1 are effective panel of markers in HBV-related HCC diagnosis in fine-needle aspiration specimens. BMC Res Notes. 2020; 13(1):388. PMC: 7450594. DOI: 10.1186/s13104-020-05230-y. View

Wang C, Shao X, Zhang X, Xie C, Yu J, Xu X . Diagnostic value of glypican-3, arginase-1 and hepatocyte paraffin antigen -1 in differentiating hepatocellular carcinoma from intrahepatic cholangiocarcinoma. Transl Cancer Res. 2022; 9(1):128-136. PMC: 8797808. DOI: 10.21037/tcr.2019.11.20. View

Petrick J, Kelly S, Altekruse S, McGlynn K, Rosenberg P . Future of Hepatocellular Carcinoma Incidence in the United States Forecast Through 2030. J Clin Oncol. 2016; 34(15):1787-94. PMC: 4966339. DOI: 10.1200/JCO.2015.64.7412. View

Timek D, Shi J, Liu H, Lin F . Arginase-1, HepPar-1, and Glypican-3 are the most effective panel of markers in distinguishing hepatocellular carcinoma from metastatic tumor on fine-needle aspiration specimens. Am J Clin Pathol. 2012; 138(2):203-10. DOI: 10.1309/AJCPK1ZC9WNHCCMU. View

10.

Weng W, Liu N, Toiyama Y, Kusunoki M, Nagasaka T, Fujiwara T . Novel evidence for a PIWI-interacting RNA (piRNA) as an oncogenic mediator of disease progression, and a potential prognostic biomarker in colorectal cancer. Mol Cancer. 2018; 17(1):16. PMC: 5791351. DOI: 10.1186/s12943-018-0767-3. View

11.

Zeng G, Zhang D, Liu X, Kang Q, Fu Y, Tang B . Co-expression of Piwil2/Piwil4 in nucleus indicates poor prognosis of hepatocellular carcinoma. Oncotarget. 2016; 8(3):4607-4617. PMC: 5354858. DOI: 10.18632/oncotarget.13491. View

12.

Lee J, Schutte D, Wulf G, Fuzesi L, Radzun H, Schweyer S . Stem-cell protein Piwil2 is widely expressed in tumors and inhibits apoptosis through activation of Stat3/Bcl-XL pathway. Hum Mol Genet. 2005; 15(2):201-11. DOI: 10.1093/hmg/ddi430. View

13.

Ferrin G, Aguilar-Melero P, Rodriguez-Peralvarez M, Montero-Alvarez J, Mata M . Biomarkers for hepatocellular carcinoma: diagnostic and therapeutic utility. Hepat Med. 2015; 7:1-10. PMC: 4403743. DOI: 10.2147/HMER.S50161. View

14.

Liu J, Shen R, Chen L, Ye Y, He G, Hua K . Piwil2 is expressed in various stages of breast cancers and has the potential to be used as a novel biomarker. Int J Clin Exp Pathol. 2010; 3(4):328-37. PMC: 2872741. View

15.

Xue R, Zhang Q, Cao Q, Kong R, Xiang X, Liu H . Liver tumour immune microenvironment subtypes and neutrophil heterogeneity. Nature. 2022; 612(7938):141-147. DOI: 10.1038/s41586-022-05400-x. View

16.

Jin L, Zhang Z, Wang Z, Tan X, Wang Z, Shen L . Novel piRNA MW557525 regulates the growth of Piwil2-iCSCs and maintains their stem cell pluripotency. Mol Biol Rep. 2022; 49(7):6957-6969. DOI: 10.1007/s11033-022-07443-9. View

17.

Al-Janabi O, Wach S, Nolte E, Weigelt K, Rau T, Stohr C . Piwi-like 1 and 4 gene transcript levels are associated with clinicopathological parameters in renal cell carcinomas. Biochim Biophys Acta. 2014; 1842(5):686-90. DOI: 10.1016/j.bbadis.2014.01.014. View

18.

Omar M, Omran M, Farid K, Tabll A, Shahein Y, Emran T . Biomarkers for Hepatocellular Carcinoma: From Origin to Clinical Diagnosis. Biomedicines. 2023; 11(7). PMC: 10377276. DOI: 10.3390/biomedicines11071852. View

19.

Fujiwara M, Kwok S, Yano H, Pai R . Arginase-1 is a more sensitive marker of hepatic differentiation than HepPar-1 and glypican-3 in fine-needle aspiration biopsies. Cancer Cytopathol. 2012; 120(4):230-7. DOI: 10.1002/cncy.21190. View

20.

Ferlay J, Colombet M, Soerjomataram I, Parkin D, Pineros M, Znaor A . Cancer statistics for the year 2020: An overview. Int J Cancer. 2021; . DOI: 10.1002/ijc.33588. View