Genome Editing Using CRISPR, CAST, and Fanzor Systems

Overview

Journal Mol Cells

Publisher Elsevier

Specialties Cell Biology
Molecular Biology

Date 2024 Jun 23

PMID 38909984

Authors

Beomjong Song

Sangsu Bae

Affiliations

Soon will be listed here.

Abstract

Genetic engineering technologies are essential not only for basic science but also for generating animal models for therapeutic applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) system, derived from adapted prokaryotic immune responses, has led to unprecedented advancements in the field of genome editing because of its ability to precisely target and edit genes in a guide RNA-dependent manner. The discovery of various types of CRISPR-Cas systems, such as CRISPR-associated transposons (CASTs), has resulted in the development of novel genome editing tools. Recently, research has expanded to systems associated with obligate mobile element guided activity (OMEGA) RNAs, including ancestral CRISPR-Cas and eukaryotic Fanzor systems, which are expected to complement the conventional CRISPR-Cas systems. In this review, we briefly introduce the features of various CRISPR-Cas systems and their application in diverse animal models.

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References

Jansen R, van Embden J, Gaastra W, Schouls L . Identification of genes that are associated with DNA repeats in prokaryotes. Mol Microbiol. 2002; 43(6):1565-75. DOI: 10.1046/j.1365-2958.2002.02839.x. View

Waaijers S, Portegijs V, Kerver J, Lemmens B, Tijsterman M, van den Heuvel S . CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans. Genetics. 2013; 195(3):1187-91. PMC: 3813849. DOI: 10.1534/genetics.113.156299. View

Zhao X, Liu L, Lang J, Cheng K, Wang Y, Li X . A CRISPR-Cas13a system for efficient and specific therapeutic targeting of mutant KRAS for pancreatic cancer treatment. Cancer Lett. 2018; 431:171-181. DOI: 10.1016/j.canlet.2018.05.042. View

Cho S, Lee J, Carroll D, Kim J, Lee J . Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Genetics. 2013; 195(3):1177-80. PMC: 3813847. DOI: 10.1534/genetics.113.155853. View

Venter J, Adams M, Myers E, Li P, Mural R, Sutton G . The sequence of the human genome. Science. 2001; 291(5507):1304-51. DOI: 10.1126/science.1058040. View

Kushawah G, Hernandez-Huertas L, Abugattas-Nunez Del Prado J, Martinez-Morales J, DeVore M, Hassan H . CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos. Dev Cell. 2020; 54(6):805-817.e7. DOI: 10.1016/j.devcel.2020.07.013. View

Buchman A, Brogan D, Sun R, Yang T, Hsu P, Akbari O . Programmable RNA Targeting Using CasRx in Flies. CRISPR J. 2020; 3(3):164-176. PMC: 7307691. DOI: 10.1089/crispr.2020.0018. View

May E, Craig N . Switching from cut-and-paste to replicative Tn7 transposition. Science. 1996; 272(5260):401-4. DOI: 10.1126/science.272.5260.401. View

Hwang W, Fu Y, Reyon D, Maeder M, Tsai S, Sander J . Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013; 31(3):227-9. PMC: 3686313. DOI: 10.1038/nbt.2501. View

10.

Hruscha A, Krawitz P, Rechenberg A, Heinrich V, Hecht J, Haass C . Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish. Development. 2013; 140(24):4982-7. DOI: 10.1242/dev.099085. View

11.

Komor A, Kim Y, Packer M, Zuris J, Liu D . Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016; 533(7603):420-4. PMC: 4873371. DOI: 10.1038/nature17946. View

12.

Vestergaard G, Garrett R, Shah S . CRISPR adaptive immune systems of Archaea. RNA Biol. 2014; 11(2):156-67. PMC: 3973734. DOI: 10.4161/rna.27990. View

13.

Karvelis T, Druteika G, Bigelyte G, Budre K, Zedaveinyte R, Silanskas A . Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease. Nature. 2021; 599(7886):692-696. PMC: 8612924. DOI: 10.1038/s41586-021-04058-1. View

14.

Ran F, Cong L, Yan W, Scott D, Gootenberg J, Kriz A . In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015; 520(7546):186-91. PMC: 4393360. DOI: 10.1038/nature14299. View

15.

Gratz S, Cummings A, Nguyen J, Hamm D, Donohue L, Harrison M . Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Genetics. 2013; 194(4):1029-35. PMC: 3730909. DOI: 10.1534/genetics.113.152710. View

16.

Vo P, Ronda C, Klompe S, Chen E, Acree C, Wang H . CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering. Nat Biotechnol. 2020; 39(4):480-489. PMC: 10583764. DOI: 10.1038/s41587-020-00745-y. View

17.

Zetsche B, Gootenberg J, Abudayyeh O, Slaymaker I, Makarova K, Essletzbichler P . Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell. 2015; 163(3):759-71. PMC: 4638220. DOI: 10.1016/j.cell.2015.09.038. View

18.

Ihry R, Worringer K, Salick M, Frias E, Ho D, Theriault K . p53 inhibits CRISPR-Cas9 engineering in human pluripotent stem cells. Nat Med. 2018; 24(7):939-946. DOI: 10.1038/s41591-018-0050-6. View

19.

Bao W, Jurka J . Homologues of bacterial TnpB_IS605 are widespread in diverse eukaryotic transposable elements. Mob DNA. 2013; 4(1):12. PMC: 3627910. DOI: 10.1186/1759-8753-4-12. View

20.

Park J, Bae S . Current status of genome editing technologies: special issue of BMB Reports in 2024. BMB Rep. 2024; 57(1):1. PMC: 10828432. View