High Coverage of Targeted Lipidomics Revealed Lipid Changes in the Follicular Fluid of Patients with Insulin-resistant Polycystic Ovary Syndrome and a Positive Correlation Between Plasmalogens and Oocyte Quality

Overview

Journal Front Endocrinol (Lausanne)

Specialty Endocrinology

Date 2024 Jun 21

PMID 38904043

Authors

Meizi Zhang

Yuanyuan Wang

Jianyong Di

Xuanlin Zhang

Ye Liu

Yixin Zhang

Bowen Li

Simeng Qi

Xiaomin Cao

Li Liu

Shouzeng Liu

Fengqin Xu

Affiliations

Soon will be listed here.

Abstract

Background: Polycystic ovary syndrome with insulin resistance (PCOS-IR) is the most common endocrine and metabolic disease in women of reproductive age, and low fertility in PCOS patients may be associated with oocyte quality; however, the molecular mechanism through which PCOS-IR affects oocyte quality remains unknown.

Methods: A total of 22 women with PCOS-IR and 23 women without polycystic ovary syndrome (control) who underwent fertilization and embryo transfer were recruited, and clinical information pertaining to oocyte quality was analyzed. Lipid components of follicular fluid (FF) were detected using high-coverage targeted lipidomics, which identified 344 lipid species belonging to 19 lipid classes. The exact lipid species associated with oocyte quality were identified.

Results: The number (rate) of two pronuclear (2PN) zygotes, the number (rate) of 2PN cleaved embryos, and the number of high-quality embryos were significantly lower in the PCOS-IR group. A total of 19 individual lipid classes and 344 lipid species were identified and quantified. The concentrations of the 19 lipid species in the normal follicular fluid (control) ranged between 10 mol/L and 10 mol/L. In addition, 39 lipid species were significantly reduced in the PCOS-IR group, among which plasmalogens were positively correlated with oocyte quality.

Conclusions: This study measured the levels of various lipids in follicular fluid, identified a significantly altered lipid profile in the FF of PCOS-IR patients, and established a correlation between poor oocyte quality and plasmalogens in PCOS-IR patients. These findings have contributed to the development of plasmalogen replacement therapy to enhance oocyte quality and have improved culture medium formulations for oocyte maturation (IVM).

Citing Articles

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Kobayashi H, Imanaka S Reprod Med Biol. 2024; 23(1):e12609.

PMID: 39351127 PMC: 11442066. DOI: 10.1002/rmb2.12609.

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