Identification of the Potato ( L.) P-type ATPase Gene Family and Investigating the Role of in Response to Pep13

Overview

Journal Front Plant Sci

Date 2024 Jun 21

PMID 38903445

Authors

Feng Zhang

Anping Yuan

Zongyue Nie

Moli Chu

Yanlin An

Affiliations

Soon will be listed here.

Abstract

P-type ATPase family members play important roles in plant growth and development and are involved in plant resistance to various biotic and abiotic factors. Extensive studies have been conducted on the P-type ATPase gene families in and rice but our understanding in potato remains relatively limited. Therefore, this study aimed to screen and analyze 48 P-type ATPase genes from the potato ( L.) genome database at the genome-wide level. Potato P-type ATPase genes were categorized into five subgroups based on the phylogenetic classification of the reported species. Additionally, several bioinformatic analyses, including gene structure analysis, chromosomal position analysis, and identification of conserved motifs and promoter cis-acting elements, were performed. Interestingly, the plasma membrane H-ATPase (PM H-ATPase) genes of one of the P3 subgroups showed differential expression in different tissues of potato. Specifically, , , and were highly expressed in the roots, whereas was expressed in potatoes only under stress. Furthermore, the small peptide Pep13 inhibited the expression of , , , and in potato roots. Transgenic plants heterologously overexpressing displayed a growth phenotype sensitive to Pep13 compared with wild-type plants. Further analysis revealed that reducing potato PM H-ATPase enzyme activity enhanced resistance to Pep13, indicating the involvement of PM H-ATPase in the physiological process of potato late blight and the enhancement of plant disease resistance. This study confirms the critical role of potato in resistance to Pep13.

Citing Articles

Identification and Analysis of the Plasma Membrane H-ATPase Gene Family in Cotton and Its Roles in Response to Salt Stress.

Cheng C, Zhang F, Li L, Ni Z Plants (Basel). 2025; 13(24.

PMID: 39771208 PMC: 11728463. DOI: 10.3390/plants13243510.

Genome-wide identification of the OVATE gene family and revelation of its expression profile and functional role in eight tissues of Rosa roxburghii Tratt.

An Y, Li X, Chen Y, Jiang S, Jing T, Zhang F BMC Plant Biol. 2024; 24(1):1068.

PMID: 39538133 PMC: 11558829. DOI: 10.1186/s12870-024-05775-1.

References

Palmgren M . PLANT PLASMA MEMBRANE H+-ATPases: Powerhouses for Nutrient Uptake. Annu Rev Plant Physiol Plant Mol Biol. 2001; 52:817-845. DOI: 10.1146/annurev.arplant.52.1.817. View

Toda Y, Wang Y, Takahashi A, Kawai Y, Tada Y, Yamaji N . Oryza sativa H+-ATPase (OSA) is Involved in the Regulation of Dumbbell-Shaped Guard Cells of Rice. Plant Cell Physiol. 2016; 57(6):1220-30. PMC: 4904443. DOI: 10.1093/pcp/pcw070. View

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

Liu L, Song W, Huang S, Jiang K, Moriwaki Y, Wang Y . Extracellular pH sensing by plant cell-surface peptide-receptor complexes. Cell. 2022; 185(18):3341-3355.e13. DOI: 10.1016/j.cell.2022.07.012. View

Contreras-Cornejo H, Macias-Rodriguez L, Alfaro-Cuevas R, Lopez-Bucio J . Trichoderma spp. Improve growth of Arabidopsis seedlings under salt stress through enhanced root development, osmolite production, and Na⁺ elimination through root exudates. Mol Plant Microbe Interact. 2014; 27(6):503-14. DOI: 10.1094/MPMI-09-13-0265-R. View

Axelsen K, Palmgren M . Inventory of the superfamily of P-type ion pumps in Arabidopsis. Plant Physiol. 2001; 126(2):696-706. PMC: 111160. DOI: 10.1104/pp.126.2.696. View

Pedersen B, Buch-Pedersen M, Morth J, Palmgren M, Nissen P . Crystal structure of the plasma membrane proton pump. Nature. 2007; 450(7172):1111-4. DOI: 10.1038/nature06417. View

Lee H, Seo Y, Lee J, Lee S, Oh S, Kim J . Plasma membrane-localized plant immune receptor targets H -ATPase for membrane depolarization to regulate cell death. New Phytol. 2021; 233(2):934-947. PMC: 9298278. DOI: 10.1111/nph.17789. View

Frias I, Caldeira M, Navarro-Avino J, Culianez-Macia F, Kuppinger O, Stransky H . A major isoform of the maize plasma membrane H(+)-ATPase: characterization and induction by auxin in coleoptiles. Plant Cell. 1996; 8(9):1533-44. PMC: 161296. DOI: 10.1105/tpc.8.9.1533. View

10.

Xu Z, Marowa P, Liu H, Du H, Zhang C, Li Y . Genome-Wide Identification and Analysis of P-Type Plasma Membrane H-ATPase Sub-Gene Family in Sunflower and the Role of and in the Development of Salt Stress Resistance. Genes (Basel). 2020; 11(4). PMC: 7231311. DOI: 10.3390/genes11040361. View

11.

Bjork P, Rasmussen S, Gjetting S, Havshoi N, Petersen T, Ipsen J . Tenuazonic acid from Stemphylium loti inhibits the plant plasma membrane H -ATPase by a mechanism involving the C-terminal regulatory domain. New Phytol. 2019; 226(3):770-784. PMC: 7187312. DOI: 10.1111/nph.16398. View

12.

Pedersen C, Axelsen K, Harper J, Palmgren M . Evolution of plant p-type ATPases. Front Plant Sci. 2012; 3:31. PMC: 3355532. DOI: 10.3389/fpls.2012.00031. View

13.

Morth J, Pedersen B, Buch-Pedersen M, Andersen J, Vilsen B, Palmgren M . A structural overview of the plasma membrane Na+,K+-ATPase and H+-ATPase ion pumps. Nat Rev Mol Cell Biol. 2010; 12(1):60-70. DOI: 10.1038/nrm3031. View

14.

Saijo Y, Loo E, Yasuda S . Pattern recognition receptors and signaling in plant-microbe interactions. Plant J. 2017; 93(4):592-613. DOI: 10.1111/tpj.13808. View

15.

Baxter I, Tchieu J, Sussman M, Boutry M, Palmgren M, Gribskov M . Genomic comparison of P-type ATPase ion pumps in Arabidopsis and rice. Plant Physiol. 2003; 132(2):618-28. PMC: 167002. DOI: 10.1104/pp.103.021923. View

16.

Oufattole M, Arango M, Boutry M . Identification and expression of three new Nicotiana plumbaginifolia genes which encode isoforms of a plasma-membrane H(+)-ATPase, and one of which is induced by mechanical stress. Planta. 2000; 210(5):715-22. DOI: 10.1007/s004250050672. View

17.

Lu J, Du J, Tian L, Li M, Zhang X, Zhang S . Divergent Response Strategies of CsABF Facing Abiotic Stress in Tea Plant: Perspectives From Drought-Tolerance Studies. Front Plant Sci. 2021; 12:763843. PMC: 8635920. DOI: 10.3389/fpls.2021.763843. View

18.

Brunner F, Rosahl S, Lee J, Rudd J, Geiler C, Kauppinen S . Pep-13, a plant defense-inducing pathogen-associated pattern from Phytophthora transglutaminases. EMBO J. 2002; 21(24):6681-8. PMC: 139088. DOI: 10.1093/emboj/cdf667. View

19.

Zhang J, Yin J, Luo J, Tang D, Zhu X, Wang J . Construction of homozygous diploid potato through maternal haploid induction. aBIOTECH. 2022; 3(3):163-168. PMC: 9590536. DOI: 10.1007/s42994-022-00080-7. View

20.

Kelley L, Mezulis S, Yates C, Wass M, Sternberg M . The Phyre2 web portal for protein modeling, prediction and analysis. Nat Protoc. 2015; 10(6):845-58. PMC: 5298202. DOI: 10.1038/nprot.2015.053. View