Screening Optimal DC-targeting Peptide to Enhance the Immune Efficacy of Recombinant Expressing RHDV VP60

Overview

Journal Virulence

Specialty Microbiology

Date 2024 Jun 20

PMID 38899573

Authors

Tian Xia

Xiao Lu

Deming Kong

Tiantian Guo

Yueyi Gao

Lingxiang Xin

Yanping Jiang

Xiaona Wang

Zhifu Shan

Jiaxuan Li

Han Zhou

Wen Cui

Xinyuan Qiao

Lijie Tang

Yijing Li

Li Wang

Affiliations

Soon will be listed here.

Abstract

Dendritic cells (DCs) present an ideal target for delivering immunogenic cargo due to their potent antigen-presenting capabilities. This targeting approach holds promise in vaccine development by enhancing the efficiency of antigen recognition and capture by DCs. To identify a high-affinity targeting peptide binding to rabbit DCs, rabbit monocyte-derived DCs (raMoDCs) were isolated and cultured, and a novel peptide, HS (HSLRHDYGYPGH), was identified using a phage-displayed peptide library. Alongside HS, two other DC-targeting peptides, KC1 and MY, previously validated in our laboratory, were employed to construct recombinant fusion-expressed rabbit hemorrhagic disease virus (RHDV) capsid protein VP60. These recombinant strains were named HS-VP60/, KC1-VP60/, and MY-VP60/. The ability of these recombinant to bind rabbit DCs was evaluated both in vivo and in vitro. Results demonstrated that the DC-targeting peptide KC1 significantly enhanced the capture efficiency of recombinant by raMoDCs, promoted DC maturation, and increased cytokine secretion. Furthermore, oral administration of KC1-VP60/ effectively induced SIgA and IgG production in rabbits, prolonged rabbit survival post-challenge, and reduced RHDV copies in organs. In summary, the DC-targeting peptide KC1 exhibited robust binding to raMoDCs, and recombinant expressing KC1-VP60 protein antigens efficiently induced systemic and mucosal immune responses in rabbits, conferring protective efficacy against RHDV. This study offers valuable insights for the development of novel RHDV vaccines.

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