Genome-Wide Identification, Characterisation, and Evolution of the Transcription Factor WRKY in Grapevine (): New View and Update

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Jun 19

PMID 38892428

Authors

Ekaterina Vodiasova

Anastasiya Sinchenko

Pavel Khvatkov

Sergey Dolgov

Affiliations

Soon will be listed here.

Abstract

WRKYs are a multigenic family of transcription factors that are plant-specific and involved in the regulation of plant development and various stress response processes. However, the evolution of genes is not fully understood. This family has also been incompletely studied in grapevine, and genes have been named with different numbers in different studies, leading to great confusion. In this work, 62 genes were identified based on six genomes of different cultivars. All genes were numbered according to their chromosomal location, and a complete revision of the numbering was performed. Amino acid variability between different cultivars was assessed for the first time and was greater than 5% for some WRKYs. According to the gene structure, all could be divided into two groups: more exons/long length and fewer exons/short length. For the first time, some chimeric genes were found in grapevine, which may play a specific role in the regulation of different processes: VvWRKY17 (an N-terminal signal peptide region followed by a non-cytoplasmic domain) and VvWRKY61 (Frigida-like domain). Five phylogenetic clades A-E were revealed and correlated with the WRKY groups (I, II, III). The evolution of was studied, and we proposed a evolution model where there were two dynamic phases of complexity and simplification in the evolution of .

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