Welfare Assessment in Pigs Using the Salivary Proteome

Overview

Journal Animals (Basel)

Date 2024 Jun 19

PMID 38891749

Authors

Sara Prims

Chris Van Ginneken

Xaveer Van Ostade

Christophe Casteleyn

Affiliations

Soon will be listed here.

Abstract

Identifying the potential presence of stress at the pig farm is fundamental since it affects pig welfare. As a result, a reliable and straightforward tool to monitor stress could record the welfare status of the animals. Although numerous methods to assess the welfare of pigs have been developed in the past, no gold standard has been established yet. Recently, the value of saliva as a tool to identify chronic stress in piglets was explored, as it can be collected fast and non-invasively. Since the protein composition, i.e., the proteome of porcine saliva, responds to stress, the affected proteins could be used as salivary stress biomarkers. The present review first defines stress and its relationship with welfare. Next, the porcine gland-specific salivary proteome is characterized. Finally, six potential salivary biomarkers for stress are proposed, i.e., odorant-binding protein, vomeromodulin-like protein, chitinase, lipocalin-1, long palate lung and nasal epithelium protein, and alpha-2-HS-glycoprotein.

References

Pineiro M, Pineiro C, Carpintero R, Morales J, Campbell F, Eckersall P . Characterisation of the pig acute phase protein response to road transport. Vet J. 2006; 173(3):669-74. DOI: 10.1016/j.tvjl.2006.02.006. View

Saco Y, Pena R, Matas-Quintanilla M, Ibanez-Lopez F, Pineiro M, Sotillo J . Influence of the circadian cycle, sex and production stage on the reference values of parameters related to stress and pathology in porcine saliva. Porcine Health Manag. 2023; 9(1):42. PMC: 10541711. DOI: 10.1186/s40813-023-00337-7. View

Issaq H, Conrads T, Janini G, Veenstra T . Methods for fractionation, separation and profiling of proteins and peptides. Electrophoresis. 2002; 23(17):3048-61. DOI: 10.1002/1522-2683(200209)23:17<3048::AID-ELPS3048>3.0.CO;2-L. View

Bosch J . The use of saliva markers in psychobiology: mechanisms and methods. Monogr Oral Sci. 2014; 24:99-108. DOI: 10.1159/000358864. View

Fuentes-Rubio M, Ceron J, de Torre C, Escribano D, Gutierrez A, Tecles F . Porcine salivary analysis by 2-dimensional gel electrophoresis in 3 models of acute stress: a pilot study. Can J Vet Res. 2014; 78(2):127-32. PMC: 3962275. View

Alonso M, Gonzalez-Montana J, Lomillos J . Consumers' Concerns and Perceptions of Farm Animal Welfare. Animals (Basel). 2020; 10(3). PMC: 7143148. DOI: 10.3390/ani10030385. View

Gutierrez A, Montes A, Gutierrez-Panizo C, Fuentes P, De la Cruz-Sanchez E . Gender influence on the salivary protein profile of finishing pigs. J Proteomics. 2017; 178:107-113. DOI: 10.1016/j.jprot.2017.11.023. View

Kulyyassov A, Fresnais M, Longuespee R . Targeted liquid chromatography-tandem mass spectrometry analysis of proteins: Basic principles, applications, and perspectives. Proteomics. 2021; 21(23-24):e2100153. DOI: 10.1002/pmic.202100153. View

Contreras-Aguilar M, Escribano D, Martinez-Subiela S, Martinez-Miro S, Rubio M, Tvarijonaviciute A . Influence of the way of reporting alpha-Amylase values in saliva in different naturalistic situations: A pilot study. PLoS One. 2017; 12(6):e0180100. PMC: 5487069. DOI: 10.1371/journal.pone.0180100. View

10.

Prims S, Van Raemdonck G, Vanden Hole C, Van Cruchten S, Van Ginneken C, Van Ostade X . On the characterisation of the porcine gland-specific salivary proteome. J Proteomics. 2019; 196:92-105. DOI: 10.1016/j.jprot.2019.01.016. View

11.

Ceron J, Contreras-Aguilar M, Escribano D, Martinez-Miro S, Lopez-Martinez M, Ortin-Bustillo A . Basics for the potential use of saliva to evaluate stress, inflammation, immune system, and redox homeostasis in pigs. BMC Vet Res. 2022; 18(1):81. PMC: 8883734. DOI: 10.1186/s12917-022-03176-w. View

12.

Walz A, Stuhler K, Wattenberg A, Hawranke E, Meyer H, Schmalz G . Proteome analysis of glandular parotid and submandibular-sublingual saliva in comparison to whole human saliva by two-dimensional gel electrophoresis. Proteomics. 2006; 6(5):1631-9. DOI: 10.1002/pmic.200500125. View

13.

Kivlighan K, Granger D, Schwartz E, Nelson V, Curran M, Shirtcliff E . Quantifying blood leakage into the oral mucosa and its effects on the measurement of cortisol, dehydroepiandrosterone, and testosterone in saliva. Horm Behav. 2004; 46(1):39-46. DOI: 10.1016/j.yhbeh.2004.01.006. View

14.

Gutierrez A, Nobauer K, Soler L, Razzazi-Fazeli E, Gemeiner M, Ceron J . Detection of potential markers for systemic disease in saliva of pigs by proteomics: a pilot study. Vet Immunol Immunopathol. 2012; 151(1-2):73-82. DOI: 10.1016/j.vetimm.2012.10.007. View

15.

Timmermans S, Souffriau J, Libert C . A General Introduction to Glucocorticoid Biology. Front Immunol. 2019; 10:1545. PMC: 6621919. DOI: 10.3389/fimmu.2019.01545. View

16.

Muneta Y, Yoshikawa T, Minagawa Y, Shibahara T, Maeda R, Omata Y . Salivary IgA as a useful non-invasive marker for restraint stress in pigs. J Vet Med Sci. 2010; 72(10):1295-300. DOI: 10.1292/jvms.10-0009. View

17.

Contreras-Aguilar M, Hevia M, Escribano D, Lamy E, Tecles F, Ceron J . Effect of food contamination and collection material in the measurement of biomarkers in saliva of horses. Res Vet Sci. 2020; 129:90-95. DOI: 10.1016/j.rvsc.2020.01.006. View

18.

Prims S, Vanden Hole C, Van Cruchten S, Van Ginneken C, Van Ostade X, Casteleyn C . Hair or salivary cortisol analysis to identify chronic stress in piglets?. Vet J. 2019; 252:105357. DOI: 10.1016/j.tvjl.2019.105357. View

19.

Hurtgen J, Leman A . Seasonal influence on the fertility of sows and gilts. J Am Vet Med Assoc. 1980; 177(7):631-5. View

20.

Prims S, Van Ostade X, Ayuso M, Dom M, Van Raemdonck G, Van Cruchten S . Chronic exposure to multiple stressors alters the salivary proteome of piglets. PLoS One. 2023; 18(5):e0286455. PMC: 10218721. DOI: 10.1371/journal.pone.0286455. View