Optimized Production and Characterization of Endo-β-mannanase by Aspergillus Niger for Generation of Prebiotic Mannooligosaccharides from Guar Gum

Overview

Journal Sci Rep

Specialty Science

Date 2024 Jun 18

PMID 38890382

Authors

Suresh Nath

Naveen Kango

Affiliations

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Abstract

Optimized production of Aspergillus niger ATCC 26011 endo-β-mannanase (ManAn) on copra meal resulted in 2.46-fold increase (10,028 U/gds). Purified ManAn (47 kDa) showed high affinity towards guar gum (GG) as compared to konjac gum and locust bean gum with K 2.67, 3.25 and 4.07 mg/mL, respectively. ManAn efficiently hydrolyzed GG and liberated mannooligosaccharides (MOS). Changes occurring in the rheological and compositional aspects of GG studied using Differential scanning calorimetry (DSC), Thermal gravimetric analysis (TGA) and X-ray diffraction (XRD) revealed increased thermal stability and crystallinity of the partially hydrolyzed guar gum (PHGG). Parametric optimization of the time and temperature dependent hydrolysis of GG (1% w/v) with 100 U/mL of ManAn at 60 °C and pH: 5.0 resulted in 12.126 mg/mL of mannotetraose (M4) in 5 min. Enhanced growth of probiotics Lactobacilli and production of short chain fatty acids (SCFA) that inhibited enteropathogens, confirmed the prebiotic potential of PHGG and M4.

Citing Articles

Discovery and Characterization of Mannan-Specialized GH5 Endo-1,4-β-mannanases: a Strategy for Açaí ( Mart.) Seeds Upgrading.

Espinheira R, Barrett K, Lange L, SantAna da Silva A, Meyer A J Agric Food Chem. 2024; 73(1):625-634.

PMID: 39680639 PMC: 11726631. DOI: 10.1021/acs.jafc.4c07018.

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