Exploring the Neuropharmacological Properties of Scopoletin-rich Extract Using and Methods

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2024 Jun 17

PMID 38883392

Authors

Shamshad Ather

Chayan Bhattacharyya

Himanshu Gupta

Yogesh Patil

Sairam Reddy Palicherla

Gauri Patil

Yasmin Khatoon

Pramodkumar P Gupta

Kapil Singh Thakur

Mansee Thakur

Affiliations

Soon will be listed here.

Abstract

Objectives: This study investigates the neuropharmacologic properties of Scopoletin, a bioactive compound in (EA) extract, for managing cognitive impairment using , , and zebrafish embryo toxicity assays.

Methods: The study estimates Scopoletin concentration in EA extract using HPTLC, assesses antioxidant properties using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ability of plasma (FRAP) assays, and uses bioinformatic tools for scopoletin targets. Zebrafish embryo toxicity (ZET) is used to assess its toxicological profile.

Results: 0.0076% w/w Scopoletin in the samples was quantified using HPTLC, further studies on the DPPH (0.5 mM) and FRAP gave EC at 440.0 μg/ml and 84.29 μg/ml respectively. Twelve common targets associated with cognitive impairment (CI) were identified, along with possible pathways and molecular interactions. Our results indicate significant binding affinities of Scopoletin with ERAP1, SCN3A, and COMT. Molecular dynamics simulations further confirm the stability of these interactions. ZET assessment demonstrated mortality after 450 µg/ml concentration of EA extract.

Conclusion: The study verifies the presence of Scopoletin in EA, along with their targets playing a crucial role in neurogenesis and neuroplasticity. The ZET demonstrated concentration-dependent effects, emphasizing the importance of dosage considerations in developing new formulations or therapeutics. This comprehensive study contributes valuable insight into the therapeutic potential of Scopoletin from EA for cognitive impairment, paving the way for further research.

References

Keiser M, Roth B, Armbruster B, Ernsberger P, Irwin J, Shoichet B . Relating protein pharmacology by ligand chemistry. Nat Biotechnol. 2007; 25(2):197-206. DOI: 10.1038/nbt1284. View

Balkrishna A, Thakur P, Varshney A . Phytochemical Profile, Pharmacological Attributes and Medicinal Properties of - A Cognitive Enhancer Herb for the Management of Neurodegenerative Etiologies. Front Pharmacol. 2020; 11:171. PMC: 7063970. DOI: 10.3389/fphar.2020.00171. View

Sethiya N, Nahata A, Singh P, Mishra S . Neuropharmacological evaluation on four traditional herbs used as nervine tonic and commonly available as Shankhpushpi in India. J Ayurveda Integr Med. 2018; 10(1):25-31. PMC: 6470306. DOI: 10.1016/j.jaim.2017.08.012. View

McWhirter L, Ritchie C, Stone J, Carson A . Functional cognitive disorders: a systematic review. Lancet Psychiatry. 2019; 7(2):191-207. DOI: 10.1016/S2215-0366(19)30405-5. View

Sethiya N, Trivedi A, Patel M, Mishra S . Comparative pharmacognostical investigation on four ethanobotanicals traditionally used as Shankhpushpi in India. J Adv Pharm Technol Res. 2012; 1(4):388-95. PMC: 3255406. DOI: 10.4103/0110-5558.76437. View

Tang D, Chen M, Huang X, Zhang G, Zeng L, Zhang G . SRplot: A free online platform for data visualization and graphing. PLoS One. 2023; 18(11):e0294236. PMC: 10635526. DOI: 10.1371/journal.pone.0294236. View

Delprato A, Xiao E, Manoj D . Connecting DCX, COMT and FMR1 in social behavior and cognitive impairment. Behav Brain Funct. 2022; 18(1):7. PMC: 9121553. DOI: 10.1186/s12993-022-00191-7. View

Heideman W, Antkiewicz D, Carney S, Peterson R . Zebrafish and cardiac toxicology. Cardiovasc Toxicol. 2005; 5(2):203-14. DOI: 10.1385/ct:5:2:203. View

Shidhi P, Nadiya F, Biju V, Vijayan S, Sasi A, Vipin C . Complete chloroplast genome of the medicinal plant : comparative analysis, identification of mutational hotspots and evolutionary dynamics with species of Solanales. Physiol Mol Biol Plants. 2021; 27(8):1867-1884. PMC: 8405790. DOI: 10.1007/s12298-021-01051-w. View

10.

Jabeen S, Ali M, Din A, Javed T, Mohammed N, Chaudhari S . Phytochemical screening and allelopathic potential of phytoextracts of three invasive grass species. Sci Rep. 2023; 13(1):8080. PMC: 10195856. DOI: 10.1038/s41598-023-35253-x. View

11.

Bartlett F, Kortmann R, Saran F . Medulloblastoma. Clin Oncol (R Coll Radiol). 2012; 25(1):36-45. DOI: 10.1016/j.clon.2012.09.008. View

12.

Farooqui A, Farooqui T, Madan A, Ong J, Ong W . Ayurvedic Medicine for the Treatment of Dementia: Mechanistic Aspects. Evid Based Complement Alternat Med. 2018; 2018:2481076. PMC: 5976976. DOI: 10.1155/2018/2481076. View

13.

Bitanihirwe B, Weber L, Feldon J, Meyer U . Cognitive impairment following prenatal immune challenge in mice correlates with prefrontal cortical AKT1 deficiency. Int J Neuropsychopharmacol. 2010; 13(8):981-96. DOI: 10.1017/S1461145710000192. View

14.

Gomathi D, Kalaiselvi M, Ravikumar G, Sophia D, Gopalakrishnan V, Uma C . Secondary metabolite credentials of Evolvulus alsinoides by high performance thin layer chromatography (HPTLC). J Biomed Res. 2013; 26(4):295-302. PMC: 3596747. DOI: 10.7555/JBR.26.20110128. View

15.

Thiagarajan S, Rama Krishnan K, Ei T, Shafie N, Arapoc D, Bahari H . Evaluation of the Effect of Aqueous Linn. Extract on Zebrafish Embryo Model through Acute Toxicity Assay Assessment. Evid Based Complement Alternat Med. 2019; 2019:9152757. PMC: 6521391. DOI: 10.1155/2019/9152757. View

16.

Ranganathan R, Haque S, Coley K, Shepheard S, Cooper-Knock J, Kirby J . Multifaceted Genes in Amyotrophic Lateral Sclerosis-Frontotemporal Dementia. Front Neurosci. 2020; 14:684. PMC: 7358438. DOI: 10.3389/fnins.2020.00684. View

17.

Mendoza F, Jimenez S . Serine/threonine kinase inhibition as antifibrotic therapy: transforming growth factor-β and Rho kinase inhibitors. Rheumatology (Oxford). 2021; 61(4):1354-1365. DOI: 10.1093/rheumatology/keab762. View

18.

Bufalieri F, Infante P, Bernardi F, Caimano M, Romania P, Moretti M . ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. Nat Commun. 2019; 10(1):3304. PMC: 6656771. DOI: 10.1038/s41467-019-11093-0. View

19.

Ding M, Qu Y, Hu B, An H . Signal pathways in the treatment of Alzheimer's disease with traditional Chinese medicine. Biomed Pharmacother. 2022; 152:113208. DOI: 10.1016/j.biopha.2022.113208. View

20.

Hannan M, Sultana A, Rahman M, Sohag A, Dash R, Uddin M . Protective Mechanisms of Nootropic Herb Shankhpushpi () against Dementia: Network Pharmacology and Computational Approach. Evid Based Complement Alternat Med. 2022; 2022:1015310. PMC: 9550454. DOI: 10.1155/2022/1015310. View