Rat Strain Differences in Seizure Frequency and Hilar Neuron Loss After Systemic Treatment with Pilocarpine

Overview

Journal Epilepsy Res

Specialty Neurology

Date 2024 Jun 16

PMID 38879905

Authors

Kristina Junghans

Megan Wyeth

Paul S Buckmaster

Affiliations

Soon will be listed here.

Abstract

At least 3 months after systemic treatment with pilocarpine to induce status epilepticus, Long-Evans and Sprague-Dawley rats were video-EEG monitored for seizures continuously for 1 month. Rats were then perfused, hippocampi were processed for Nissl staining, and hilar neurons were quantified. Seizure frequency in Long-Evans rats was 1/10th of that in Sprague-Dawley rats, and more variable. Hilar neuron loss was also less severe in Long-Evans rats. However, there was no correlation between hilar neuron loss and seizure frequency in either strain. The low and variable seizure frequency suggests limited usefulness of pilocarpine-treated Long-Evans rats for some epilepsy experiments.

References

Bertram E, Cornett J . The evolution of a rat model of chronic spontaneous limbic seizures. Brain Res. 1994; 661(1-2):157-62. DOI: 10.1016/0006-8993(94)91192-4. View

Passarelli V, Martins Castro L . Gender and age influence in daytime and nighttime seizure occurrence in epilepsy associated with mesial temporal sclerosis. Epilepsy Behav. 2015; 50:14-7. DOI: 10.1016/j.yebeh.2015.05.028. View

Karoly P, Nurse E, Freestone D, Ung H, Cook M, Boston R . Bursts of seizures in long-term recordings of human focal epilepsy. Epilepsia. 2017; 58(3):363-372. PMC: 5339053. DOI: 10.1111/epi.13636. View

Ramgopal S, Powell C, Zarowski M, Alexopoulos A, Kothare S, Loddenkemper T . Predicting diurnal and sleep/wake seizure patterns in paediatric patients of different ages. Epileptic Disord. 2014; 16(1):56-66. DOI: 10.1684/epd.2014.0644. View

Gorter J, van Vliet E, Aronica E, Lopes da Silva F . Progression of spontaneous seizures after status epilepticus is associated with mossy fibre sprouting and extensive bilateral loss of hilar parvalbumin and somatostatin-immunoreactive neurons. Eur J Neurosci. 2001; 13(4):657-69. DOI: 10.1046/j.1460-9568.2001.01428.x. View

MARGERISON J, Corsellis J . Epilepsy and the temporal lobes. A clinical, electroencephalographic and neuropathological study of the brain in epilepsy, with particular reference to the temporal lobes. Brain. 1966; 89(3):499-530. DOI: 10.1093/brain/89.3.499. View

Golden G, Smith G, Ferraro T, Reyes P . Rat strain and age differences in kainic acid induced seizures. Epilepsy Res. 1995; 20(2):151-9. DOI: 10.1016/0920-1211(94)00079-c. View

Herman S, Walczak T, Bazil C . Distribution of partial seizures during the sleep--wake cycle: differences by seizure onset site. Neurology. 2001; 56(11):1453-9. DOI: 10.1212/wnl.56.11.1453. View

Haut S, Shinnar S, Moshe S . Seizure clustering: risks and outcomes. Epilepsia. 2005; 46(1):146-9. DOI: 10.1111/j.0013-9580.2005.29004.x. View

10.

Golden G, Smith G, Ferraro T, Reyes P, Kulp J, Fariello R . Strain differences in convulsive response to the excitotoxin kainic acid. Neuroreport. 1991; 2(3):141-4. DOI: 10.1097/00001756-199103000-00008. View

11.

West M, Slomianka L, Gundersen H . Unbiased stereological estimation of the total number of neurons in thesubdivisions of the rat hippocampus using the optical fractionator. Anat Rec. 1991; 231(4):482-97. DOI: 10.1002/ar.1092310411. View

12.

Pavlova M, Shea S, Bromfield E . Day/night patterns of focal seizures. Epilepsy Behav. 2004; 5(1):44-9. DOI: 10.1016/j.yebeh.2003.10.013. View

13.

Loscher W, Cramer S, Ebert U . Differences in kindling development in seven outbred and inbred rat strains. Exp Neurol. 1999; 154(2):551-9. DOI: 10.1006/exnr.1998.6948. View

14.

Cavalheiro E, Leite J, Bortolotto Z, Turski W, Ikonomidou C, Turski L . Long-term effects of pilocarpine in rats: structural damage of the brain triggers kindling and spontaneous recurrent seizures. Epilepsia. 1991; 32(6):778-82. DOI: 10.1111/j.1528-1157.1991.tb05533.x. View

15.

Racine R, Burnham W, Gartner J, Levitan D . Rates of motor seizure development in rats subjected to electrical brain stimulation: strain and inter-stimulation interval effects. Electroencephalogr Clin Neurophysiol. 1973; 35(5):553-6. DOI: 10.1016/0013-4694(73)90033-3. View

16.

Buckmaster P, Dudek F . Neuron loss, granule cell axon reorganization, and functional changes in the dentate gyrus of epileptic kainate-treated rats. J Comp Neurol. 1997; 385(3):385-404. View

17.

Raedt R, Van Dycke A, Van Melkebeke D, De Smedt T, Claeys P, Wyckhuys T . Seizures in the intrahippocampal kainic acid epilepsy model: characterization using long-term video-EEG monitoring in the rat. Acta Neurol Scand. 2009; 119(5):293-303. DOI: 10.1111/j.1600-0404.2008.01108.x. View

18.

Williams P, White A, Clark S, Ferraro D, Swiercz W, Staley K . Development of spontaneous recurrent seizures after kainate-induced status epilepticus. J Neurosci. 2009; 29(7):2103-12. PMC: 2897752. DOI: 10.1523/JNEUROSCI.0980-08.2009. View

19.

Spencer D, Sun F, Brown S, Jobst B, Fountain N, Wong V . Circadian and ultradian patterns of epileptiform discharges differ by seizure-onset location during long-term ambulatory intracranial monitoring. Epilepsia. 2016; 57(9):1495-502. DOI: 10.1111/epi.13455. View

20.

Quigg M, Clayburn H, Straume M, Menaker M, Bertram 3rd E . Effects of circadian regulation and rest-activity state on spontaneous seizures in a rat model of limbic epilepsy. Epilepsia. 2000; 41(5):502-9. DOI: 10.1111/j.1528-1157.2000.tb00202.x. View