Development of Self-compatible Chinese Cabbage Lines of Chiifu Through Marker-assisted Selection

Overview

Journal Front Plant Sci

Date 2024 Jun 14

PMID 38872891

Authors

Lichun Chang

Jianli Liang

Xu Cai

Li Zhang

Yufang Li

Jian Wu

Xiaowu Wang

Affiliations

Soon will be listed here.

Abstract

The continuously refined genome assembly of the Chinese cabbage accession Chiifu is widely recognized as the reference for . However, the high self-incompatibility of Chiifu limits its broader utilization. In this study, we report the development of self-compatible Chiifu lines through a meticulous marker-assisted selection (MAS) strategy, involving the substitution of the Chiifu allele of () with that from the self-compatible Yellow Sarson (YS). A YS-based marker (SC-MLPK) was employed to screen 841 accessions, confirming that all eight accessions with the () genotype exhibited self-compatibility. Additionally, we designed 131 High-Resolution Melting (HRM) markers evenly distributed across the genome as genomic background selection (GBS) markers to facilitate the introgression of self-compatibility from YS into Chiifu along with SC-MLPK. Genome background screening revealed that the BCS population had a proportion of the recurrent parent genome (PR) ranging from 93.9% to 98.5%. From this population, we identified self-compatible individuals exhibiting a high number of pollen tubes penetrating stigmas (NPT) (>25) and a maximum compatibility index (CI) value of 7.5. Furthermore, we selected two individuals demonstrating significant similarity to Chiifu in both genetic background and morphological appearance, alongside self-compatibility. These selected individuals were self-pollinated to generate two novel lines designated as SC-Chiifu Lines. The development of these self-compatible Chiifu lines, together with the SC-MLPK marker and the set of HRM markers, represents valuable tools for genetics and breeding.

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