Optimal Design of Synthetic Circular RNAs

Overview

Journal Exp Mol Med

Specialties Biochemistry
Molecular Biology

Date 2024 Jun 13

PMID 38871815

Authors

Seo-Won Choi

Jin-Wu Nam

Affiliations

Soon will be listed here.

Abstract

Circular RNAs are an unusual class of single-stranded RNAs whose ends are covalently linked via back-splicing. Due to their versatility, the need to express circular RNAs in vivo and in vitro has increased. Efforts have been made to efficiently and precisely synthesize circular RNAs. However, a review on the optimization of the processes of circular RNA design, synthesis, and delivery is lacking. Our review highlights the multifaceted aspects considered when producing optimal circular RNAs and summarizes the available options for each step of exogenous circular RNA design and synthesis, including circularization strategies. Additionally, this review describes several potential applications of circular RNAs.

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