DHM/SERS Reveals Cellular Morphology and Molecular Changes During IPSCs-derived Activation of Astrocytes

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2024 Jun 13

PMID 38867782

Authors

Xiaoya Bu

Liwei Yang

Xianxin Han

Shengde Liu

Xiaoxu Lu

Jianhui Wan

Xiao Zhang

Ping Tang

Weina Zhang

Liyun Zhong

Affiliations

Soon will be listed here.

Abstract

The activation of astrocytes derived from induced pluripotent stem cells (iPSCs) is of great significance in neuroscience research, and it is crucial to obtain both cellular morphology and biomolecular information non-destructively in situ, which is still complicated by the traditional optical microscopy and biochemical methods such as immunofluorescence and western blot. In this study, we combined digital holographic microscopy (DHM) and surface-enhanced Raman scattering (SERS) to investigate the activation characteristics of iPSCs-derived astrocytes. It was found that the projected area of activated astrocytes decreased by 67%, while the cell dry mass increased by 23%, and the cells changed from a flat polygonal shape to an elongated star-shaped morphology. SERS analysis further revealed an increase in the intensities of protein spectral peaks (phenylalanine 1001 cm, proline 1043 cm, etc.) and lipid-related peaks (phosphatidylserine 524 cm, triglycerides 1264 cm, etc.) decreased in intensity. Principal component analysis-linear discriminant analysis (PCA-LDA) modeling based on spectral data distinguished resting and reactive astrocytes with a high accuracy of 96.5%. The increase in dry mass correlated with the increase in protein content, while the decrease in projected area indicated the adjustment of lipid composition and cell membrane remodeling. Importantly, the results not only reveal the cellular morphology and molecular changes during iPSCs-derived astrocytes activation but also reflect their mapping relationship, thereby providing new insights into diagnosing and treating neurodegenerative diseases.

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