Strong Interaction of CpcL with Photosystem I Cores Induced in Heterocysts of Sp. PCC 7120

Overview

Journal MicroPubl Biol

Specialty Biology

Date 2024 Jun 12

PMID 38863983

Authors

Takehiro Suzuki

Haruya Ogawa

Naoshi Dohmae

Jian-Ren Shen

Shigeki Ehira

Ryo Nagao

Affiliations

Soon will be listed here.

Abstract

Phycobilisomes (PBSs) are photosynthetic light-harvesting antennae and appear to be loosely bound to photosystem I (PSI). We previously found unique protein bands in each PSI fraction in heterocysts of sp. PCC 7120 by two-dimensional blue native/SDS-PAGE; however, the protein bands have not been identified. Here we analyzed the protein bands by mass spectrometry, which were identified as CpcL, one of the components in PBSs. As different composition and organization of PSI-PBS supercomplexes were observed, the expression and binding properties of PBSs including CpcL to PSIs in this cyanobacterium may be diversified in response to its living environments.

References

Adir N, Bar-Zvi S, Harris D . The amazing phycobilisome. Biochim Biophys Acta Bioenerg. 2019; 1861(4):148047. DOI: 10.1016/j.bbabio.2019.07.002. View

Watanabe M, Ikeuchi M . Phycobilisome: architecture of a light-harvesting supercomplex. Photosynth Res. 2013; 116(2-3):265-76. DOI: 10.1007/s11120-013-9905-3. View

Hirose Y, Misawa N, Yonekawa C, Nagao N, Watanabe M, Ikeuchi M . Characterization of the genuine type 2 chromatic acclimation in the two Geminocystis cyanobacteria. DNA Res. 2017; 24(4):387-396. PMC: 5737509. DOI: 10.1093/dnares/dsx011. View

Nagao R, Yokono M, Ueno Y, Nakajima Y, Suzuki T, Kato K . Excitation-energy transfer in heterocysts isolated from the cyanobacterium Anabaena sp. PCC 7120 as studied by time-resolved fluorescence spectroscopy. Biochim Biophys Acta Bioenerg. 2021; 1863(1):148509. DOI: 10.1016/j.bbabio.2021.148509. View

MacColl . Cyanobacterial phycobilisomes . J Struct Biol. 1999; 124(2-3):311-34. DOI: 10.1006/jsbi.1998.4062. View

Nelson N, Junge W . Structure and energy transfer in photosystems of oxygenic photosynthesis. Annu Rev Biochem. 2015; 84:659-83. DOI: 10.1146/annurev-biochem-092914-041942. View

Watanabe M, Semchonok D, Webber-Birungi M, Ehira S, Kondo K, Narikawa R . Attachment of phycobilisomes in an antenna-photosystem I supercomplex of cyanobacteria. Proc Natl Acad Sci U S A. 2014; 111(7):2512-7. PMC: 3932850. DOI: 10.1073/pnas.1320599111. View

Magnuson A, Cardona T . Thylakoid membrane function in heterocysts. Biochim Biophys Acta. 2015; 1857(3):309-19. DOI: 10.1016/j.bbabio.2015.10.016. View

Brettel K, Leibl W . Electron transfer in photosystem I. Biochim Biophys Acta. 2001; 1507(1-3):100-14. DOI: 10.1016/s0005-2728(01)00202-x. View

10.

Shimizu S, Ogawa H, Tsuboshita N, Suzuki T, Kato K, Nakajima Y . Tight association of CpcL with photosystem I in Anabaena sp. PCC 7120 grown under iron-deficient conditions. Biochim Biophys Acta Bioenerg. 2023; 1864(4):148993. DOI: 10.1016/j.bbabio.2023.148993. View

11.

Adir N . Elucidation of the molecular structures of components of the phycobilisome: reconstructing a giant. Photosynth Res. 2005; 85(1):15-32. DOI: 10.1007/s11120-004-2143-y. View

12.

Guo R, Xu Y, Zhu J, Scheer H, Zhao K . Assembly of CpcL-phycobilisomes. Plant J. 2024; 118(4):1207-1217. DOI: 10.1111/tpj.16666. View

13.

Bryant D, Stirewalt V, Glauser M, FRANK G, Sidler W, Zuber H . A small multigene family encodes the rod-core linker polypeptides of Anabaena sp. PCC7120 phycobilisomes. Gene. 1991; 107(1):91-9. DOI: 10.1016/0378-1119(91)90301-q. View

14.

Nagao R, Kato K, Suzuki T, Ifuku K, Uchiyama I, Kashino Y . Structural basis for energy harvesting and dissipation in a diatom PSII-FCPII supercomplex. Nat Plants. 2019; 5(8):890-901. DOI: 10.1038/s41477-019-0477-x. View

15.

Fromme P, Jordan P, Krauss N . Structure of photosystem I. Biochim Biophys Acta. 2001; 1507(1-3):5-31. DOI: 10.1016/s0005-2728(01)00195-5. View

16.

Nagao R, Yokono M, Ueno Y, Suzuki T, Kato K, Kato K . Molecular organizations and function of iron-stress-induced-A protein family in Anabaena sp. PCC 7120. Biochim Biophys Acta Bioenerg. 2020; 1862(1):148327. DOI: 10.1016/j.bbabio.2020.148327. View

17.

You X, Zhang X, Cheng J, Xiao Y, Ma J, Sun S . In situ structure of the red algal phycobilisome-PSII-PSI-LHC megacomplex. Nature. 2023; 616(7955):199-206. DOI: 10.1038/s41586-023-05831-0. View

18.

Zheng L, Zhang Z, Wang H, Zheng Z, Wang J, Liu H . Cryo-EM and femtosecond spectroscopic studies provide mechanistic insight into the energy transfer in CpcL-phycobilisomes. Nat Commun. 2023; 14(1):3961. PMC: 10322944. DOI: 10.1038/s41467-023-39689-7. View

19.

Chang L, Liu X, Li Y, Liu C, Yang F, Zhao J . Structural organization of an intact phycobilisome and its association with photosystem II. Cell Res. 2015; 25(6):726-37. PMC: 4456626. DOI: 10.1038/cr.2015.59. View

20.

Rast A, Schaffer M, Albert S, Wan W, Pfeffer S, Beck F . Biogenic regions of cyanobacterial thylakoids form contact sites with the plasma membrane. Nat Plants. 2019; 5(4):436-446. DOI: 10.1038/s41477-019-0399-7. View