Red2Flpe-SCON: a Versatile, Multicolor Strategy for Generating Mosaic Conditional Knockout Mice

Overview

Journal Nat Commun

Specialty Biology

Date 2024 Jun 11

PMID 38862535

Authors

Szu-Hsien Sam Wu

Somi Kim

Heetak Lee

Ji-Hyun Lee

So-Yeon Park

Reka Bakonyi

Isaree Teriyapirom

Natalia Hallay

Sandra Pilat-Carotta

Hans-Christian Theussl

Jihoon Kim

Joo-Hyeon Lee

Benjamin D Simons

Jong Kyoung Kim

Gabriele Colozza

Bon-Kyoung Koo

Affiliations

Soon will be listed here.

Abstract

Image-based lineage tracing enables tissue turnover kinetics and lineage potentials of different adult cell populations to be investigated. Previously, we reported a genetic mouse model system, Red2Onco, which ectopically expressed mutated oncogenes together with red fluorescent proteins (RFP). This system enabled the expansion kinetics and neighboring effects of oncogenic clones to be dissected. We now report Red2Flpe-SCON: a mosaic knockout system that uses multicolor reporters to label both mutant and wild-type cells. We develop the Red2Flpe mouse line for red clone-specific Flpe expression, as well as the FRT-based SCON (Short Conditional IntrON) method to facilitate tunable conditional mosaic knockouts in mice. We use the Red2Flpe-SCON method to study Sox2 mutant clonal analysis in the esophageal epithelium of adult mice which reveal that the stem cell gene, Sox2, is less essential for adult stem cell maintenance itself, but rather for stem cell proliferation and differentiation.

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