Laboratory Rearing for -Based Control Programs, a Component of Dengue Control

Overview

Journal J Arthropod Borne Dis

Publisher Tehran University of Medical Sciences

Date 2024 Jun 11

PMID 38860195

Authors

Nazanin Naseri-Karimi

Hassan Vatandoost

Mohammad Mehdi Sedaghat

Seyed Hassan Moosa-Kazemi

Fardin Amidi

Mohammad Ali Oshaghi

Affiliations

Soon will be listed here.

Abstract

Background: flies are smooth, low upkeep and safe model organisms, they can be effortlessly used in different fields of life sciences like genomics, biotechnology, genetics, disease model, and -based approaches to fight vectors and the pathogens they transmit.

Methods: Fruit fly specimens were collected in 25 districts (14 provinces) of Iran and their morphological recognition was proven by molecular analysis based on sequence homology of mitochondrial COI barcode region. Essential information and specific requirements ere provided for laboratory rearing of .

Results: colonies were found in 23 out of 25 districts. Also, five related species coincident with were reported in this study including , , (Diptera: Drosophilidae), and (Diptera: Phoridae). The Iranian molecular signature and their rearing techniques have been described here. The complete life cycle, from (egg to adult), takes approximately 8 days at 25 °C. Some biological points have been presented with highlighting capturing, rearing, culturing, and embryo collection along with primitive recognition and segregation between females and males have been presented. A recipe for culture media and the quantity of various ingredients have been provided.

Conclusion: This is the first report on the and species for the country. Results of this study provide efficient and effective rearing procedures which are requirement for both small-scale for facilitating entomological research and large-scale use in justifiable vector control management such as disease model or Dengue control.

Citing Articles

and Infesting : Detection by Quantitative PCR, Genotyping, and Involvement in the Transmission of Microbial Pathogens.

Rossi F, Iannitto M, Hulaj B, Manocchio P, Gentile F, Matto I Insects. 2024; 15(10).

PMID: 39452362 PMC: 11508623. DOI: 10.3390/insects15100786.

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