Isolation of Short RNAs with Homogeneous 3'-ends Using Quaternary-amine Anion Exchange Chromatography

Overview

Journal Biol Methods Protoc

Publisher Oxford University Press

Specialty Biology

Date 2024 Jun 10

PMID 38855193

Authors

Zixian Li

Mia Bilic

Bhushan Nagar

Affiliations

Soon will be listed here.

Abstract

Visualizing RNA-protein interactions through structural approaches requires the use of RNA molecules purified to homogeneity. We describe here a simple and effective method, free of acrylamide contamination and without using UV radiation, to separate synthesized, heterogeneous RNA transcripts (up to ∼15 nucleotides) at single-nucleotide resolution by quaternary-amine anion exchange chromatography. The quality of short RNAs isolated through this method is validated by gel electrophoresis, mass spectrometry, and crystallization with a protein-binding partner.

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