What's the Situation with Ocular Inflammation? A Cross-seasonal Investigation of Proteomic Changes in Ocular Allergy Sufferers' Tears in Victoria, Australia

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2024 Jun 10

PMID 38855108

Authors

Esrin Aydin

Shuai Nie

Serap Azizoglu

Luke Chong

Moneisha Gokhale

Cenk Suphioglu

Affiliations

Soon will be listed here.

Abstract

Background: Ocular allergy (OA) is a localized subset of allergy characterized by ocular surface itchiness, redness and inflammation. Inflammation and eye-rubbing, due to allergy-associated itch, are common in OA sufferers and may trigger changes to the ocular surface biochemistry. The primary aim of this study is to assess the differences in the human tear proteome between OA sufferers and Healthy Controls (HCs) across peak allergy season and off-peak season in Victoria, Australia.

Methods: 19 participants (14 OA sufferers, 5 HCs) aged 18-45 were recruited for this study. Participants were grouped based on allergy symptom assessment questionnaire scoring. Proteins were extracted from human tear samples and were run on an Orbitrap Mass Spectrometer. Peaks were matched to a DIA library. Data was analyzed using the software MaxQuant, Perseus and IBM SPSS.

Results: 1267 proteins were identified in tear samples of OA sufferers and HCs. 23 proteins were differentially expressed between peak allergy season OA suffers vs HCs, and 21 were differentially expressed in off-peak season. Decreased proteins in OA sufferers related to cell structure regulation, inflammatory regulation and antimicrobial regulation. In both seasons, OA sufferers were shown to have increased expression of proteins relating to inflammation, immune responses and cellular development.

Conclusion: Tear protein identification showed dysregulation of proteins involved in inflammation, immunity and cellular structures. Proteins relating to cellular structure may suggest a possible link between OA-associated itch and the subsequent ocular surface damage via eye-rubbing, while inflammatory and immune protein changes highlight potential diagnostic and therapeutic biomarkers of OA.

References

Grimm C, Kraft R, Sauerbruch S, Schultz G, Harteneck C . Molecular and functional characterization of the melastatin-related cation channel TRPM3. J Biol Chem. 2003; 278(24):21493-501. DOI: 10.1074/jbc.M300945200. View

Tibrewal S, Ivanir Y, Sarkar J, Nayeb-Hashemi N, Bouchard C, Kim E . Hyperosmolar stress induces neutrophil extracellular trap formation: implications for dry eye disease. Invest Ophthalmol Vis Sci. 2014; 55(12):7961-9. PMC: 4263134. DOI: 10.1167/iovs.14-15332. View

Wu H, Wang Y, Dong N, Yang F, Lin Z, Shang X . Meibomian gland dysfunction determines the severity of the dry eye conditions in visual display terminal workers. PLoS One. 2014; 9(8):e105575. PMC: 4140788. DOI: 10.1371/journal.pone.0105575. View

McMonnies C . Eye rubbing type and prevalence including contact lens 'removal-relief' rubbing. Clin Exp Optom. 2016; 99(4):366-72. DOI: 10.1111/cxo.12343. View

Aydin E, Dhar P, Gokhale M, Chong L, Azizoglu S, Suphioglu C . A Review of Emerging Tear Proteomics Research on the Ocular Surface in Ocular Allergy. Biology (Basel). 2022; 11(2). PMC: 8869539. DOI: 10.3390/biology11020312. View

Lopez-Lopez M, Regueiro U, Bravo S, Chantada-Vazquez M, Pena C, Diez-Feijoo E . Shotgun Proteomics for the Identification and Profiling of the Tear Proteome of Keratoconus Patients. Invest Ophthalmol Vis Sci. 2022; 63(5):12. PMC: 9123485. DOI: 10.1167/iovs.63.5.12. View

Chen D, Zheng J, Gerasimcik N, Lagerstedt K, Sjogren H, Abrahamsson J . The Expression Pattern of the Pre-B Cell Receptor Components Correlates with Cellular Stage and Clinical Outcome in Acute Lymphoblastic Leukemia. PLoS One. 2016; 11(9):e0162638. PMC: 5017602. DOI: 10.1371/journal.pone.0162638. View

Li K, Liu X, Chen Z, Huang Q, Wu K . Quantification of tear proteins and sPLA2-IIa alteration in patients with allergic conjunctivitis. Mol Vis. 2010; 16:2084-91. PMC: 2965574. View

Leonardi A, Borghesan F, Faggian D, Depaoli M, Secchi A, Plebani M . Tear and serum soluble leukocyte activation markers in conjunctival allergic diseases. Am J Ophthalmol. 2000; 129(2):151-8. DOI: 10.1016/s0002-9394(99)00295-0. View

10.

Sun F, Bi Q, Wang X, Liu J . Down-regulation of mir-27b promotes angiogenesis and fibroblast activation through activating PI3K/AKT signaling pathway. Wound Repair Regen. 2019; 28(1):39-48. DOI: 10.1111/wrr.12765. View

11.

Li B, Sheng M, Li J, Yan G, Lin A, Li M . Tear proteomic analysis of Sjögren syndrome patients with dry eye syndrome by two-dimensional-nano-liquid chromatography coupled with tandem mass spectrometry. Sci Rep. 2014; 4:5772. PMC: 4145314. DOI: 10.1038/srep05772. View

12.

McMonnies C . Mechanisms of rubbing-related corneal trauma in keratoconus. Cornea. 2009; 28(6):607-15. DOI: 10.1097/ICO.0b013e318198384f. View

13.

Stoltz D, Jackson D, Evans M, Gangnon R, Tisler C, Gern J . Specific patterns of allergic sensitization in early childhood and asthma & rhinitis risk. Clin Exp Allergy. 2013; 43(2):233-41. PMC: 3557802. DOI: 10.1111/cea.12050. View

14.

Blom A . The role of complement inhibitors beyond controlling inflammation. J Intern Med. 2017; 282(2):116-128. DOI: 10.1111/joim.12606. View

15.

Peck A, Ambrus Jr J . A Temporal Comparative RNA Transcriptome Profile of the Annexin Gene Family in the Salivary versus Lacrimal Glands of the Sjögren's Syndrome-Susceptible C57BL/6.NOD- Mouse. Int J Mol Sci. 2022; 23(19). PMC: 9570365. DOI: 10.3390/ijms231911709. View

16.

Doecke J, Laws S, Faux N, Wilson W, Burnham S, Lam C . Blood-based protein biomarkers for diagnosis of Alzheimer disease. Arch Neurol. 2012; 69(10):1318-25. PMC: 6287606. DOI: 10.1001/archneurol.2012.1282. View

17.

Gemenetzi K, Psomopoulos F, Carriles A, Gounari M, Minici C, Plevova K . Higher-order immunoglobulin repertoire restrictions in CLL: the illustrative case of stereotyped subsets 2 and 169. Blood. 2020; 137(14):1895-1904. DOI: 10.1182/blood.2020005216. View

18.

Pannebaker C, Chandler H, Nichols J . Tear proteomics in keratoconus. Mol Vis. 2010; 16:1949-57. PMC: 2956673. View

19.

Aluru S, Agarwal S, Srinivasan B, Iyer G, Rajappa S, Tatu U . Lacrimal proline rich 4 (LPRR4) protein in the tear fluid is a potential biomarker of dry eye syndrome. PLoS One. 2012; 7(12):e51979. PMC: 3525644. DOI: 10.1371/journal.pone.0051979. View

20.

Yenihayat F, Altintas O, Kasap M, Akpinar G, Guzel N, Celik O . Comparative proteome analysis of the tear samples in patients with low-grade keratoconus. Int Ophthalmol. 2017; 38(5):1895-1905. DOI: 10.1007/s10792-017-0672-6. View