First Report of Amphotericin B Resistant Isolated from the ICU of a Referral Hospital in Indonesia

Overview

Journal Iran J Microbiol

Publisher Tehran University of Medical Sciences

Specialty Infectious Diseases

Date 2024 Jun 10

PMID 38854988

Authors

Rifdah Hanifah

Mardiastuti Wahid

Andi Yasmon

Affiliations

Soon will be listed here.

Abstract

Background And Objectives: Amphotericin B is a broad-spectrum antifungal agent commonly used to treat infection. was isolated from patients reported to be intrinsically resistant to amphotericin B, encoded by the ERG2 and ERG11 genes. However, there have been limited studies concerning amphotericin B-resistant in Indonesia. The objective of this study is to explore the phenotypic and genotypic characteristics (ERG2 and ERG11) of isolated from the ICU of a referral hospital in Indonesia.

Materials And Methods: Identification and susceptibility tests were conducted using VITEK2. Thereafter, DNA was extracted and amplified using conventional PCR followed by DNA sequencing (Sanger method).

Results: The results of the phenotypic susceptibility test showed that all were resistant to amphotericin B. ERG2 and ERG11 sequences showed the same amino acid sequence and corresponded to references that are resistant to amphotericin B.

Conclusion: The resistant properties of against amphotericin B found in this study require further exploration, including comparing resistant and sensitive to amphotericin B. In addition, it is necessary to analyze other genes besides ERG2 and ERG11.

Citing Articles

The multidrug-resistant complex and phylogenetic related species: Insights into antifungal resistance mechanisms.

Ramos L, Barbosa P, Lorentino C, Lima J, Braga A, Lima R Curr Res Microb Sci. 2025; 8:100354.

PMID: 39995443 PMC: 11847750. DOI: 10.1016/j.crmicr.2025.100354.

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