Inhibition of Insulin Secretion Induces Golgi Morphological Changes

Overview

Journal Juntendo Iji Zasshi

Specialty General Medicine

Date 2024 Jun 10

PMID 38854847

Authors

Tatsuya Iwamoto

Shigeomi Shimizu

Hajime Tajima-Sakurai

Hirofumi Yamaguchi

Yuya Nishida

Satoko Arakawa

Hirotaka Watada

Affiliations

Soon will be listed here.

Abstract

Objectives: The role of autophagy in pancreatic β cells has been reported, but the relationship between autophagy and insulin metabolism is complex and is not fully understood yet.

Design: We here analyze the relationship between autophagy and insulin metabolism from a morphological aspect.

Methods: We observe the morphological changes of β cell-specific Atg7-deficient mice and Atg5-deficient MIN6 cells with electron microscopy.

Results: We find that Atg7-deficient β cells exhibit a marked expansion of the endoplasmic reticulum (ER). We also find that the inhibitory state of insulin secretion causes morphological changes in the Golgi, including ministacking and swelling. The same morphological alterations are observed when insulin secretion is suppressed in Atg5-deficient MIN6 cells.

Conclusions: The defect of autophagy induces ER expansion, and inhibition of insulin secretion induces Golgi swelling, probably via ER stress and Golgi stress, respectively.

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