Poloxamer-Based Mixed Micelles Loaded with Thymol or Eugenol for Topical Applications

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2024 Jun 10

PMID 38854547

Authors

Jana Sedlarikova

Magda Janalikova

Pavlina Egner

Pavel Pleva

Affiliations

Soon will be listed here.

Abstract

Poloxamers (P184, P188, and P407) have been investigated as the carrier system for eugenol or thymol. A synergic effect of mixed Poloxamers was proved by enhanced micellar parameters, with a lower critical micelle concentration (about 0.06 mM) and the highest surface adsorption of 9 × 10 mol m for P188/P407. Dynamic light scattering revealed a decrease in micellar size after loading with biomolecules. Three mathematical models were applied to study the release kinetics, of which Korsmeyer-Peppas was the best fitted model. Higher relative release was observed for Poloxamer/eugenol samples, up to a value of 0.8. Poloxamer micelles with thymol were highly influential in bacterial reduction. Single P407/eugenol micelles proved to be bacteriostatic for up to 6 h for or up to 48 h for . Mixed micelles were confirmed to have prolonged bacteriostatic activity for up to 72 h against both bacteria. This trend was also proven by the modified Gompertz model. An optimized P188/P407/eugenol micelle was successfully used as a model system for release study with a particle size of less than 30 nm and high encapsulation efficiency surpassing 90%. The developed mixed micelles were proved to have antibiofilm activity, and thus they provide an innovative approach for controlled release with potential in topical applications.

References

Walsh S, Maillard J, Russell A, Catrenich C, Charbonneau D, Bartolo R . Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria. J Appl Microbiol. 2003; 94(2):240-7. DOI: 10.1046/j.1365-2672.2003.01825.x. View

Piombino C, Lange H, Sabuzi F, Galloni P, Conte V, Crestini C . Lignosulfonate Microcapsules for Delivery and Controlled Release of Thymol and Derivatives. Molecules. 2020; 25(4). PMC: 7070466. DOI: 10.3390/molecules25040866. View

Di Spirito N, Grizzuti N, Lutz-Bueno V, Urciuoli G, Auriemma F, Pasquino R . Pluronic F68 Micelles as Carriers for an Anti-Inflammatory Drug: A Rheological and Scattering Investigation. Langmuir. 2024; 40(2):1544-1554. PMC: 10795184. DOI: 10.1021/acs.langmuir.3c03682. View

Mod Razif M, Chan S, Widodo R, Chew Y, Hassan M, Ahmad Hisham S . Optimization of a Luteolin-Loaded TPGS/Poloxamer 407 Nanomicelle: The Effects of Copolymers, Hydration Temperature and Duration, and Freezing Temperature on Encapsulation Efficiency, Particle Size, and Solubility. Cancers (Basel). 2023; 15(14). PMC: 10378229. DOI: 10.3390/cancers15143741. View

Lambert R, Skandamis P, Coote P, Nychas G . A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol. 2001; 91(3):453-62. DOI: 10.1046/j.1365-2672.2001.01428.x. View

Ulloa P, Guarda A, Valenzuela X, Rubilar J, Galotto M . Modeling the release of antimicrobial agents (thymol and carvacrol) from two different encapsulation materials. Food Sci Biotechnol. 2018; 26(6):1763-1772. PMC: 6049722. DOI: 10.1007/s10068-017-0226-8. View

Darpentigny C, Marcoux P, Menneteau M, Michel B, Ricoul F, Jean B . Antimicrobial Cellulose Nanofibril Porous Materials Obtained by Supercritical Impregnation of Thymol. ACS Appl Bio Mater. 2022; 3(5):2965-2975. DOI: 10.1021/acsabm.0c00033. View

Zhu Z, Min T, Zhang X, Wen Y . Microencapsulation of Thymol in Poly(lactide-co-glycolide) (PLGA): Physical and Antibacterial Properties. Materials (Basel). 2019; 12(7). PMC: 6480635. DOI: 10.3390/ma12071133. View

Vivero-Lopez M, Sparacino C, Quelle-Regaldie A, Sanchez L, Candal E, Barreiro-Iglesias A . Pluronic®/casein micelles for ophthalmic delivery of resveratrol: In vitro, ex vivo, and in vivo tests. Int J Pharm. 2022; 628:122281. DOI: 10.1016/j.ijpharm.2022.122281. View

10.

Siskova A, Pleva P, Hruza J, Frajova J, Sedlarikova J, Peer P . Reuse of Textile Waste to Production of the Fibrous Antibacterial Membrane with Filtration Potential. Nanomaterials (Basel). 2022; 12(1). PMC: 8746662. DOI: 10.3390/nano12010050. View

11.

Sotoudegan F, Amini M, Faizi M, Aboofazeli R . Nimodipine-Loaded Pluronic Block Copolymer Micelles: Preparation, Characterization, and Studies. Iran J Pharm Res. 2017; 15(4):641-661. PMC: 5316244. View

12.

Das A, Nanda P, Bandyopadhyay S, Banerjee R, Biswas S, McClements D . Application of nanoemulsion-based approaches for improving the quality and safety of muscle foods: A comprehensive review. Compr Rev Food Sci Food Saf. 2020; 19(5):2677-2700. DOI: 10.1111/1541-4337.12604. View

13.

Prasanthan P, Kishore N . Self-assemblies of pluronic micelles in partitioning of anticancer drugs and effectiveness of this system towards target protein. RSC Adv. 2022; 11(36):22057-22069. PMC: 9034178. DOI: 10.1039/d1ra03770f. View

14.

Sedlarikova J, Janalikova M, Peer P, Pavlatkova L, Minarik A, Pleva P . Zein-Based Films Containing Monolaurin/Eugenol or Essential Oils with Potential for Bioactive Packaging Application. Int J Mol Sci. 2022; 23(1). PMC: 8745270. DOI: 10.3390/ijms23010384. View

15.

Wang L, Zhang Y . Eugenol Nanoemulsion Stabilized with Zein and Sodium Caseinate by Self-Assembly. J Agric Food Chem. 2017; 65(14):2990-2998. DOI: 10.1021/acs.jafc.7b00194. View

16.

An S, Ban E, Chung I, Cho Y, Kim A . Antimicrobial Activities of Propolis in Poloxamer Based Topical Gels. Pharmaceutics. 2021; 13(12). PMC: 8706605. DOI: 10.3390/pharmaceutics13122021. View

17.

Garcia-Salinas S, Gamez E, Landa G, Arruebo M, Irusta S, Mendoza G . Antimicrobial Wound Dressings against Fluorescent and Methicillin-Sensitive Intracellular Pathogenic Bacteria. ACS Appl Mater Interfaces. 2020; 12(46):51302-51313. DOI: 10.1021/acsami.0c17043. View

18.

Pleva P, Bartosova L, Macalova D, Zalesakova L, Sedlarikova J, Janalikova M . Biofilm Formation Reduction by Eugenol and Thymol on Biodegradable Food Packaging Material. Foods. 2022; 11(1). PMC: 8750975. DOI: 10.3390/foods11010002. View

19.

Namivandi-Zangeneh R, Yang Y, Xu S, Wong E, Boyer C . Antibiofilm Platform based on the Combination of Antimicrobial Polymers and Essential Oils. Biomacromolecules. 2019; 21(1):262-272. DOI: 10.1021/acs.biomac.9b01278. View

20.

Du E, Gan L, Li Z, Wang W, Liu D, Guo Y . In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens. J Anim Sci Biotechnol. 2015; 6:58. PMC: 4690362. DOI: 10.1186/s40104-015-0055-7. View