Evaluation of Bovine Embryo Quality Based on Gene Expression Profiling Using Whole-transcriptome Amplification

Overview

Journal J Reprod Dev

Specialties Biology
Reproductive Medicine

Date 2024 Jun 9

PMID 38852998

Authors

Takashi Fujii

Takamasa Mukai

Shoji Hasegawa

Toh-Ichi Hirata

Ken Sawai

Affiliations

Soon will be listed here.

Abstract

This study aimed to develop a method to evaluate the quality of bovine in vitro fertilized (IVF) embryos based on gene expression profiling via whole-transcriptome amplification. The expression of 11 developmentally important genes in individual bovine in vivo-derived (IVD) and IVF embryos were examined. Gene expression profiling was conducted by classifying the expression level of each gene in individual embryos as low, medium, or high. The IVF group had a higher (P < 0.01) proportion of embryos with low expression of SOX2, NANOG, and FGF4. In addition, a correlation analysis between the expression levels of each gene in individual embryos demonstrated that the relationship between gene expression differed with respect to IVD and IVF embryos. Our results suggest that the expression profiling of developmentally important genes using IVD embryos as normal controls could be a useful indicator for evaluating the quality of bovine IVF embryos.

References

Khurana N, Niemann H . Energy metabolism in preimplantation bovine embryos derived in vitro or in vivo. Biol Reprod. 2000; 62(4):847-56. DOI: 10.1095/biolreprod62.4.847. View

Canepa M, Ortega N, Monteleone M, Mucci N, Kaiser G, Brocco M . Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos. PLoS One. 2014; 9(9):e108139. PMC: 4182429. DOI: 10.1371/journal.pone.0108139. View

Aguila L, Osycka-Salut C, Treulen F, Felmer R . Pluripotent Core in Bovine Embryos: A Review. Animals (Basel). 2022; 12(8). PMC: 9032358. DOI: 10.3390/ani12081010. View

Luo L, Shi Y, Wang H, Wang Z, Dang Y, Li S . Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency. PLoS Genet. 2022; 18(7):e1010307. PMC: 9286228. DOI: 10.1371/journal.pgen.1010307. View

de A Camargo L, Powell A, do Vale Filho V, Wall R . Comparison of gene expression in individual preimplantation bovine embryos produced by in vitro fertilisation or somatic cell nuclear transfer. Reprod Fertil Dev. 2005; 17(5):487-96. DOI: 10.1071/rd04128. View

Driver A, Penagaricano F, Huang W, Ahmad K, Hackbart K, Wiltbank M . RNA-Seq analysis uncovers transcriptomic variations between morphologically similar in vivo- and in vitro-derived bovine blastocysts. BMC Genomics. 2012; 13:118. PMC: 3368723. DOI: 10.1186/1471-2164-13-118. View

Siqueira L, Torres C, Souza E, Monteiro Jr P, Arashiro E, Camargo L . Pregnancy rates and corpus luteum-related factors affecting pregnancy establishment in bovine recipients synchronized for fixed-time embryo transfer. Theriogenology. 2009; 72(7):949-58. DOI: 10.1016/j.theriogenology.2009.06.013. View

Tsuiko O, Catteeuw M, Esteki M, Destouni A, Pascottini O, Besenfelder U . Genome stability of bovine in vivo-conceived cleavage-stage embryos is higher compared to in vitro-produced embryos. Hum Reprod. 2017; 32(11):2348-2357. DOI: 10.1093/humrep/dex286. View

Chitwood J, Rincon G, Kaiser G, Medrano J, Ross P . RNA-seq analysis of single bovine blastocysts. BMC Genomics. 2013; 14:350. PMC: 3668197. DOI: 10.1186/1471-2164-14-350. View

10.

Ealy A, Wooldridge L, McCoski S . BOARD INVITED REVIEW: Post-transfer consequences of in vitro-produced embryos in cattle. J Anim Sci. 2019; 97(6):2555-2568. PMC: 6541818. DOI: 10.1093/jas/skz116. View

11.

Lazzari G, Wrenzycki C, Herrmann D, Duchi R, Kruip T, Niemann H . Cellular and molecular deviations in bovine in vitro-produced embryos are related to the large offspring syndrome. Biol Reprod. 2002; 67(3):767-75. DOI: 10.1095/biolreprod.102.004481. View

12.

Noguchi T, Aizawa T, Munakata Y, Iwata H . Comparison of gene expression and mitochondria number between bovine blastocysts obtained and . J Reprod Dev. 2019; 66(1):35-39. PMC: 7040209. DOI: 10.1262/jrd.2019-100. View

13.

Ortega M, Kelleher A, ONeil E, Benne J, Cecil R, Spencer T . NANOG is required to form the epiblast and maintain pluripotency in the bovine embryo. Mol Reprod Dev. 2019; 87(1):152-160. PMC: 6983337. DOI: 10.1002/mrd.23304. View

14.

Jiang Z, Sun J, Dong H, Luo O, Zheng X, Obergfell C . Transcriptional profiles of bovine in vivo pre-implantation development. BMC Genomics. 2014; 15:756. PMC: 4162962. DOI: 10.1186/1471-2164-15-756. View

15.

Corcoran D, Fair T, Park S, Rizos D, Patel O, Smith G . Suppressed expression of genes involved in transcription and translation in in vitro compared with in vivo cultured bovine embryos. Reproduction. 2006; 131(4):651-60. DOI: 10.1530/rep.1.01015. View

16.

Sawai K, Kageyama S, Moriyasu S, Hirayama H, Minamihashi A, Onoe S . Analysis of mRNA transcripts for insulin-like growth factor receptors and binding proteins in bovine embryos derived from somatic cell nuclear transfer. Cloning Stem Cells. 2005; 7(3):189-98. DOI: 10.1089/clo.2005.7.189. View

17.

Fujii T, Moriyasu S, Hirayama H, Hashizume T, Sawai K . Aberrant expression patterns of genes involved in segregation of inner cell mass and trophectoderm lineages in bovine embryos derived from somatic cell nuclear transfer. Cell Reprogram. 2010; 12(5):617-25. DOI: 10.1089/cell.2010.0017. View

18.

Pontes J, Nonato-Junior I, Sanches B, Ereno-Junior J, Uvo S, Barreiros T . Comparison of embryo yield and pregnancy rate between in vivo and in vitro methods in the same Nelore (Bos indicus) donor cows. Theriogenology. 2008; 71(4):690-7. DOI: 10.1016/j.theriogenology.2008.09.031. View

19.

Smith S, Everts R, Sung L, Du F, Page R, Henderson B . Gene expression profiling of single bovine embryos uncovers significant effects of in vitro maturation, fertilization and culture. Mol Reprod Dev. 2008; 76(1):38-47. DOI: 10.1002/mrd.20927. View

20.

Tanaka S, Kunath T, Hadjantonakis A, Nagy A, Rossant J . Promotion of trophoblast stem cell proliferation by FGF4. Science. 1998; 282(5396):2072-5. DOI: 10.1126/science.282.5396.2072. View