Chemical Compositions of Eucalyptus Sp. Essential Oils and the Evaluation of Their Combinations As a Promising Treatment Against Ear Bacterial Infections

Overview

Journal BMC Complement Med Ther

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2024 Jun 7

PMID 38849805

Authors

Elaissi Ameur

Moumni Sarra

Derbali Yosra

Khouja Mariem

Abid Nabil

Jlasssi Ibrahim

Khaloud Mohammed Alarjani

Frederic Lynen

Khouja Mohamed Larbi

Affiliations

Soon will be listed here.

Abstract

Background: The chemical composition and biological activities of Eucalyptus essential oils (EOs) have been documented in numerous studies against multiple infectious diseases. The antibacterial activity of individual Eucalyptus EOs against strains that cause ear infections was investigated in our previous study. The study's antibacterial activity was promising, which prompted us to explore this activity further with EO blends.

Methods: We tested 15 combinations (9 binary combinations and 6 combinations of binary combinations) of Eucalyptus EOs extracted by hydrodistillation from eight Tunisian Eucalyptus species dried leaves against six bacterial strains responsible for ear infections: three bacterial isolates (Haemophilus influenzae, Haemophilus parainfluenzae, and Klebsiella pneumoniae) and three reference bacteria strains (Pseudomonas aeruginosa, ATTC 9027; Staphylococcus aureus, ATCC 6538; and Escherichia coli, ATCC 8739). The EOs were analyzed using GC/FID and GC/MS. The major compounds, as well as all values obtained from the bacterial growth inhibition assay, were utilized for statistical analysis.

Results: The antibacterial activity of the EO blends exhibited significant variation within Eucalyptus species, bacterial strains, and the applied methods. Principal component analysis (PCA) and hierarchical cluster analysis (HCA), based on the diameters of the inhibition zone, facilitated the identification of two major groups and ten subgroups based on the level of antibacterial activity. The highest antibacterial activity was observed for the mixture of EOs extracted from E. panctata, E. accedens, and E. cladoclayx (paac) as well as E. panctata, E. wandoo, E. accedens, and E. cladoclayx (pwac) using the disc diffusion method. Additionally, significant activity was noted with EOs extracted from E. panctata, E. wandoo (pw) and E. panctata, E. accedens (pa) using the broth microdilution method.

Conclusion: Our findings suggest that certain EO combinations (paac, pwac, pw, and pa) could be considered as potential alternative treatment for ear infections due to their demonstrated highly promising antibacterial activities.

References

Pandey A, Kumar P, Singh P, Tripathi N, Bajpai V . Essential Oils: Sources of Antimicrobials and Food Preservatives. Front Microbiol. 2017; 7:2161. PMC: 5238431. DOI: 10.3389/fmicb.2016.02161. View

Hailu D, Mekonnen D, Derbie A, Mulu W, Abera B . Pathogenic bacteria profile and antimicrobial susceptibility patterns of ear infection at Bahir Dar Regional Health Research Laboratory Center, Ethiopia. Springerplus. 2016; 5:466. PMC: 4833760. DOI: 10.1186/s40064-016-2123-7. View

Winnett V, Sirdaarta J, White A, Clarke F, Cock I . Inhibition of Klebsiella pneumoniae growth by selected Australian plants: natural approaches for the prevention and management of ankylosing spondylitis. Inflammopharmacology. 2017; 25(2):223-235. DOI: 10.1007/s10787-017-0328-1. View

Bhat A, Ahangar A . Methods for detecting chemical-chemical interaction in toxicology. Toxicol Mech Methods. 2009; 17(8):441-50. DOI: 10.1080/15376510601177654. View

Fandino I, Fernandez-Turren G, Ferret A, Moya D, Castillejos L, Calsamiglia S . Exploring Additive, Synergistic or Antagonistic Effects of Natural Plant Extracts on In Vitro Beef Feedlot-Type Rumen Microbial Fermentation Conditions. Animals (Basel). 2020; 10(1). PMC: 7022539. DOI: 10.3390/ani10010173. View

Ghaly M, Shaheen A, Bouhy A, Bendary M . Alternative therapy to manage otitis media caused by multidrug-resistant fungi. Arch Microbiol. 2020; 202(5):1231-1240. DOI: 10.1007/s00203-020-01832-z. View

Ameur E, Sarra M, Yosra D, Mariem K, Nabil A, Lynen F . Chemical composition of essential oils of eight Tunisian Eucalyptus species and their antibacterial activity against strains responsible for otitis. BMC Complement Med Ther. 2021; 21(1):209. PMC: 8359536. DOI: 10.1186/s12906-021-03379-y. View

Panahi Y, Akhavan A, Sahebkar A, Hosseini S, Taghizadeh M, Akbari H . Investigation of the effectiveness of Syzygium aromaticum, Lavandula angustifolia and Geranium robertianum essential oils in the treatment of acute external otitis: a comparative trial with ciprofloxacin. J Microbiol Immunol Infect. 2013; 47(3):211-6. DOI: 10.1016/j.jmii.2012.10.002. View

Hendry E, Worthington T, Conway B, Lambert P . Antimicrobial efficacy of eucalyptus oil and 1,8-cineole alone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilm cultures. J Antimicrob Chemother. 2009; 64(6):1219-25. DOI: 10.1093/jac/dkp362. View

10.

Mulyaningsih S, Sporer F, Reichling J, Wink M . Antibacterial activity of essential oils from Eucalyptus and of selected components against multidrug-resistant bacterial pathogens. Pharm Biol. 2011; 49(9):893-9. DOI: 10.3109/13880209.2011.553625. View

11.

Ayari S, Shankar S, Follett P, Hossain F, Lacroix M . Potential synergistic antimicrobial efficiency of binary combinations of essential oils against Bacillus cereus and Paenibacillus amylolyticus-Part A. Microb Pathog. 2020; 141:104008. DOI: 10.1016/j.micpath.2020.104008. View

12.

Becerril R, Nerin C, Gomez-Lus R . Evaluation of bacterial resistance to essential oils and antibiotics after exposure to oregano and cinnamon essential oils. Foodborne Pathog Dis. 2012; 9(8):699-705. DOI: 10.1089/fpd.2011.1097. View

13.

Argaw-Denboba A, Abejew A, Mekonnen A . Antibiotic-Resistant Bacteria Are Major Threats of Otitis Media in Wollo Area, Northeastern Ethiopia: A Ten-Year Retrospective Analysis. Int J Microbiol. 2016; 2016:8724671. PMC: 4745944. DOI: 10.1155/2016/8724671. View

14.

Ouattara B, Simard R, Holley R, Piette G, Begin A . Antibacterial activity of selected fatty acids and essential oils against six meat spoilage organisms. Int J Food Microbiol. 1997; 37(2-3):155-62. DOI: 10.1016/s0168-1605(97)00070-6. View

15.

Nazzaro F, Fratianni F, De Martino L, Coppola R, De Feo V . Effect of essential oils on pathogenic bacteria. Pharmaceuticals (Basel). 2013; 6(12):1451-74. PMC: 3873673. DOI: 10.3390/ph6121451. View

16.

Fadil M, Fikri-Benbrahim K, Rachiq S, Ihssane B, Lebrazi S, Chraibi M . Combined treatment of Thymus vulgaris L., Rosmarinus officinalis L. and Myrtus communis L. essential oils against Salmonella typhimurium: Optimization of antibacterial activity by mixture design methodology. Eur J Pharm Biopharm. 2017; 126:211-220. DOI: 10.1016/j.ejpb.2017.06.002. View

17.

Kachkoul R, Benjelloun Touimi G, Bennani B, El Habbani R, El Mouhri G, Mohim M . The Synergistic Effect of Three Essential Oils against Bacteria Responsible for the Development of Lithiasis Infection: An Optimization by the Mixture Design. Evid Based Complement Alternat Med. 2021; 2021:1305264. PMC: 8421168. DOI: 10.1155/2021/1305264. View

18.

Bassole I, Juliani H . Essential oils in combination and their antimicrobial properties. Molecules. 2012; 17(4):3989-4006. PMC: 6268925. DOI: 10.3390/molecules17043989. View

19.

Yap P, Yiap B, Ping H, Lim S . Essential oils, a new horizon in combating bacterial antibiotic resistance. Open Microbiol J. 2014; 8:6-14. PMC: 3950955. DOI: 10.2174/1874285801408010006. View

20.

Hyldgaard M, Mygind T, Meyer R . Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front Microbiol. 2012; 3:12. PMC: 3265747. DOI: 10.3389/fmicb.2012.00012. View