Endophyte-mediated Enhancement of Salt Resistance in L. by Regulation of Osmotic Stress and Plant Defense-related Genes

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Jun 7

PMID 38846577

Authors

Qihua Liang

Dedong Tan

Haohai Chen

Xiaoli Guo

Muhammad Afzal

Xiaolin Wang

Zhiyuan Tan

Guixiang Peng

Affiliations

Soon will be listed here.

Abstract

Introduction: Soil salinization poses a significant environmental challenge affecting plant growth and agricultural sustainability. This study explores the potential of salt-tolerant endophytes to mitigate the adverse effects of soil salinization, emphasizing their impact on the development and resistance of L. (peanuts).

Methods: The diversity of culturable plant endophytic bacteria associated with was investigated. The study focused on the effects of , , and on the development and germination of seeds in pots subjected to high NaCl concentrations (200 mM L).

Results: Under elevated NaCl concentrations, the inoculation of endophytes significantly ( < 0.05) enhanced seedling germination and increased the activities of enzymes such as Superoxide dismutase, catalase, and polyphenol oxidase, while reducing malondialdehyde and peroxidase levels. Additionally, endophyte inoculation resulted in increased root surface area, plant height, biomass contents, and leaf surface area of peanuts under NaCl stress. Transcriptome data revealed an augmented defense and resistance response induced by the applied endophyte (, , and ) strain, including upregulation of abiotic stress related mechanisms such as fat metabolism, hormones, and glycosyl inositol phosphorylceramide (Na receptor). Na receptor under salt stress gate Ca influx channels in plants. Notably, the synthesis of secondary metabolites, especially genes related to terpene and phenylpropanoid pathways, was highly regulated.

Conclusion: The inoculated endophytes played a possible role in enhancing salt tolerance in peanuts. Future investigations should explore protein-protein interactions between plants and endophytes to unravel the mechanisms underlying endophyte-mediated salt resistance in plants.

References

Oh Y, Ahn C, Nam K, Kim Y, Yoon N, Je J . Amino Acid Composition, Antioxidant, and Cytoprotective Effect of Blue Mussel () Hydrolysate through the Inhibition of Caspase-3 Activation in Oxidative Stress-Mediated Endothelial Cell Injury. Mar Drugs. 2019; 17(2). PMC: 6409750. DOI: 10.3390/md17020135. View

Chaudhry V, Patil P . Genomic investigation reveals evolution and lifestyle adaptation of endophytic Staphylococcus epidermidis. Sci Rep. 2016; 6:19263. PMC: 4713051. DOI: 10.1038/srep19263. View

Singh A . Soil salinization management for sustainable development: A review. J Environ Manage. 2020; 277:111383. DOI: 10.1016/j.jenvman.2020.111383. View

Ali B, Wang X, Saleem M, Azeem M, Afridi M, Nadeem M . PM35 Reinforces Photosynthetic Efficiency, Antioxidant Defense, Expression of Stress-Responsive Genes, and Ameliorates the Effects of Salinity Stress in Maize. Life (Basel). 2022; 12(2). PMC: 8875943. DOI: 10.3390/life12020219. View

Li M, Wang Y, Guo C, Wang S, Zheng L, Bu Y . The claim of primacy of human gut in dietary cellobiose degradation. Gut Microbes. 2023; 15(1):2227434. PMC: 10291918. DOI: 10.1080/19490976.2023.2227434. View

Hidri R, Metoui-Ben Mahmoud O, Zorrig W, Mahmoudi H, Smaoui A, Abdelly C . Plant Growth-Promoting Rhizobacteria Alleviate High Salinity Impact on the Halophyte by Modulating Antioxidant Defense and Soil Biological Activity. Front Plant Sci. 2022; 13:821475. PMC: 9199488. DOI: 10.3389/fpls.2022.821475. View

Ravi B, Foyer C, Pandey G . The integration of reactive oxygen species (ROS) and calcium signalling in abiotic stress responses. Plant Cell Environ. 2023; 46(7):1985-2006. DOI: 10.1111/pce.14596. View

Kopczewski T, Kuzniak E, Kornas A, Rut G, Nosek M, Ciereszko I . Local and Systemic Changes in Photosynthetic Parameters and Antioxidant Activity in Cucumber Challenged with pv . Int J Mol Sci. 2020; 21(17). PMC: 7504232. DOI: 10.3390/ijms21176378. View

Ahmed U, Pfannstiel J, Stressler T, Eisele T . Purification and characterization of a fungal aspartic peptidase from Trichoderma reesei and its application for food and animal feed protein hydrolyses. J Sci Food Agric. 2022; 102(12):5190-5199. DOI: 10.1002/jsfa.11871. View

10.

Lung S, Lai S, Wang H, Zhang X, Liu A, Guo Z . Oxylipin signaling in salt-stressed soybean is modulated by ligand-dependent interaction of Class II acyl-CoA-binding proteins with lipoxygenase. Plant Cell. 2021; 34(3):1117-1143. PMC: 8894927. DOI: 10.1093/plcell/koab306. View

11.

Egamberdieva D, Wirth S, Bellingrath-Kimura S, Mishra J, Arora N . Salt-Tolerant Plant Growth Promoting Rhizobacteria for Enhancing Crop Productivity of Saline Soils. Front Microbiol. 2020; 10:2791. PMC: 6930159. DOI: 10.3389/fmicb.2019.02791. View

12.

Ganie S, Wani S, Henry R, Hensel G . Improving rice salt tolerance by precision breeding in a new era. Curr Opin Plant Biol. 2021; 60:101996. DOI: 10.1016/j.pbi.2020.101996. View

13.

Li L, Qi Q, Zhang H, Dong Q, Iqbal A, Gui H . Ameliorative Effects of Silicon against Salt Stress in L. Antioxidants (Basel). 2022; 11(8). PMC: 9404900. DOI: 10.3390/antiox11081520. View

14.

Garcia-Abellan J, Albaladejo I, Egea I, Flores F, Capel C, Capel J . The phenotype alterations showed by the res tomato mutant disappear when the plants are grown under semi-arid conditions: Is the res mutant tolerant to multiple stresses?. Plant Signal Behav. 2016; 12(11):e1146847. PMC: 5703232. DOI: 10.1080/15592324.2016.1146847. View

15.

Banik N, Bhattacharjee S . Complementation of ROS scavenging secondary metabolites with enzymatic antioxidant defense system augments redox-regulation property under salinity stress in rice. Physiol Mol Biol Plants. 2020; 26(8):1623-1633. PMC: 7415070. DOI: 10.1007/s12298-020-00844-9. View

16.

Asen N, Aluko R . Acetylcholinesterase and butyrylcholinesterase inhibitory activities of antioxidant peptides obtained from enzymatic pea protein hydrolysates and their ultrafiltration peptide fractions. J Food Biochem. 2022; 46(11):e14289. DOI: 10.1111/jfbc.14289. View

17.

Pal K, Dey R, Sherathia D, Devidayal , Mangalassery S, Kumar A . Alleviation of Salinity Stress in Peanut by Application of Endophytic Bacteria. Front Microbiol. 2021; 12:650771. PMC: 8079962. DOI: 10.3389/fmicb.2021.650771. View

18.

Pan L, Hu X, Liao L, Xu T, Sun Q, Tang M . Lipid metabolism and antioxidant system contribute to salinity tolerance in halophytic grass seashore paspalum in a tissue-specific manner. BMC Plant Biol. 2023; 23(1):337. PMC: 10290340. DOI: 10.1186/s12870-023-04358-w. View

19.

Khan N, Ali S, Shahid M, Mustafa A, Sayyed R, Cura J . Insights into the Interactions among Roots, Rhizosphere, and Rhizobacteria for Improving Plant Growth and Tolerance to Abiotic Stresses: A Review. Cells. 2021; 10(6). PMC: 8234610. DOI: 10.3390/cells10061551. View

20.

Li J, Hu L, Zhang L, Pan X, Hu X . Exogenous spermidine is enhancing tomato tolerance to salinity-alkalinity stress by regulating chloroplast antioxidant system and chlorophyll metabolism. BMC Plant Biol. 2015; 15:303. PMC: 4696305. DOI: 10.1186/s12870-015-0699-7. View