Escherichia Coli Mutants Possessing an Li+-resistant Melibiose Carrier

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1985 Apr 1

PMID 3884586

Citations 5

Authors

S Shiota

Y Yamane

M Futai

T Tsuchiya

Affiliations

Soon will be listed here.

Abstract

Escherichia coli K-12 strains in the absence of the lactose carrier grew on the disaccharide melibiose as the sole source of carbon. The presence of 0.1 mM Li+ in the medium strongly inhibited growth of such cells, and Li+-resistant mutants appeared after several days of incubation. These mutants showed altered cation coupling to melibiose transport via the melibiose carrier. Cotransport between H+ and melibiose was lost in the mutants, although Na+-melibiose cotransport was retained. We observed no Li+-melibiose cotransport. Therefore, these mutants represent a new type of cation-coupling mutants of the melibiose carrier.

Citing Articles

Arg-52 in the melibiose carrier of Escherichia coli is important for cation-coupled sugar transport and participates in an intrahelical salt bridge.

Franco P, Wilson T J Bacteriol. 1999; 181(20):6377-86.

PMID: 10515928 PMC: 103773. DOI: 10.1128/JB.181.20.6377-6386.1999.

Proline transport in Salmonella typhimurium: putP permease mutants with altered substrate specificity.

Dila D, Maloy S J Bacteriol. 1986; 168(2):590-4.

PMID: 3536852 PMC: 213521. DOI: 10.1128/jb.168.2.590-594.1986.

Dissecting the molecular mechanism of ion-solute cotransport: substrate specificity mutations in the putP gene affect the kinetics of proline transport.

MYERS R, Townsend D, Maloy S J Membr Biol. 1991; 121(3):201-14.

PMID: 1865487 DOI: 10.1007/BF01951554.

Asp-51 and Asp-120 are important for the transport function of the Escherichia coli melibiose carrier.

Wilson D, Wilson T J Bacteriol. 1992; 174(9):3083-6.

PMID: 1569035 PMC: 205965. DOI: 10.1128/jb.174.9.3083-3086.1992.

Cloning and sequencing of the melB gene encoding the melibiose permease of Salmonella typhimurium LT2.

Mizushima K, Awakihara S, Kuroda M, Ishikawa T, Tsuda M, Tsuchiya T Mol Gen Genet. 1992; 234(1):74-80.

PMID: 1495487 DOI: 10.1007/BF00272347.

References

Tanaka S, Lerner S, Lin E . Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol. J Bacteriol. 1967; 93(2):642-8. PMC: 276489. DOI: 10.1128/jb.93.2.642-648.1967. View

Umeda K, Shiota S, Futai M, Tsuchiya T . Inhibitory effect of Li+ on cell growth and pyruvate kinase activity of Escherichia coli. J Bacteriol. 1984; 160(2):812-4. PMC: 214814. DOI: 10.1128/jb.160.2.812-814.1984. View

Tsuchiya T, Wilson T . Cation-sugar cotransport in the melibiose transport system of Escherichia coli. Membr Biochem. 1978; 2(1):63-79. DOI: 10.3109/09687687809063858. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Stock J, Roseman S . A sodium-dependent sugar co-transport system in bacteria. Biochem Biophys Res Commun. 1971; 44(1):132-8. DOI: 10.1016/s0006-291x(71)80168-7. View

Wilson T, Kusch M . A mutant of Escherichia coli K 12 energy-uncoupled for lactose transport. Biochim Biophys Acta. 1972; 255(3):786-97. DOI: 10.1016/0005-2736(72)90391-4. View

West I, Wilson T . Galactoside transport dissociated from proton movement in mutants of Escherichia coli. Biochem Biophys Res Commun. 1973; 50(2):551-8. DOI: 10.1016/0006-291x(73)90875-9. View

Tsuchiya T, Raven J, Wilson T . Co-transport of Na+ and methul-beta-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coli. Biochem Biophys Res Commun. 1977; 76(1):26-31. DOI: 10.1016/0006-291x(77)91663-1. View

Schuldiner S, Fishkes H . Sodium-proton antiport in isolated membrane vesicles of Escherichia coli. Biochemistry. 1978; 17(4):706-11. DOI: 10.1021/bi00597a023. View

10.

Lopilato J, Tsuchiya T, Wilson T . Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli. J Bacteriol. 1978; 134(1):147-56. PMC: 222229. DOI: 10.1128/jb.134.1.147-156.1978. View

11.

Tsuchiya T, Lopilato J, Wilson T . Effect of lithium ion on melibiose transport in Escherichia coli. J Membr Biol. 1978; 42(1):45-59. DOI: 10.1007/BF01870393. View

12.

Tanaka K, Niiya S, Tsuchiya T . Melibiose transport of Escherichia coli. J Bacteriol. 1980; 141(3):1031-6. PMC: 293773. DOI: 10.1128/jb.141.3.1031-1036.1980. View

13.

Niiya S, Yamasaki K, Wilson T, Tsuchiya T . Altered cation coupling to melibiose transport in mutants of Escherichia coli. J Biol Chem. 1982; 257(15):8902-6. View

14.

Tsuchiya T, Oho M . Lithium ion-sugar cotransport via the melibiose transport system in Escherichia coli. Measurement of Li+ transport and specificity. J Biol Chem. 1983; 258(21):12765-7. View

15.

Yazyu H, Shimamoto T, Kanazawa H, Futai M, Tsuchiya T . Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. J Biol Chem. 1984; 259(7):4320-6. View

16.

Shiota S, Yazyu H, Tsuchiya T . Escherichia coli mutants with altered cation recognition by the melibiose carrier. J Bacteriol. 1984; 160(1):445-7. PMC: 214741. DOI: 10.1128/jb.160.1.445-447.1984. View